Pulsed electric field (PEF)-assisted protein recovery from Chlorella vulgaris is mediated by an enzymatic process after cell death

Scherer, Daniel; Krust, Damaris; Frey, Wolfgang; Mueller, Georg; Nick, Peter; Gusbeth, Christian

doi:10.1016/j.algal.2019.101536

Cited by 49 publications

(27 citation statements)

References 31 publications

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“…(Goettel et al, 2013) have also recommended an incubation time of at least 2 h post-PEF treatment to ensure sufficient diffusion of Auxenochlorella protothecoides. Influence of temperature on the release of proteins during post-PEF incubation have been investigated in C. vulgaris (Scherer et al, 2019). Consistently, their findings also revealed high protein release at 20-40 °C.…”

Section: Effect Of Incubation Temperature On Protein and C-phycocyanimentioning

confidence: 64%

“…Although PEF as a treatment method has the potential for protein or C-Phycocyanin recovery for industrial applications, upscaling of the process has to be achievable. The influence of the biomass concentration on protein extraction efficiency after PEF treatment has been shown on Chlorella vulgaris (Scherer et al, 2019). They have shown maximum protein recovery from PEF treated C. vulgaris using diluted biomass (2.5 mg•ml -1 ), whereas increasing the biomass concentration from 2.5 to 12.5 mg•ml -1 reduced the protein extraction efficiency from 50% to 30% of total protein content.…”

Section: Introductionmentioning

confidence: 99%

“…Since C-Phycocyanin are water-soluble proteins located on the outer membrane of thylakoids (Safaei et al, 2019), PEF treatment can greatly enhance their release into the suspension. Indeed, (Scherer et al, 2019) have also shown that the extraction of membrane-bound proteins by PEF treatment are often limited, whereas water-soluble proteins can be extracted very efficiently. In the current study, irreversible PEF treatment has been conducted as a pre-treatment method for the extraction of proteins and C-Phycocyanin from A. platensis.…”

Section: Introductionmentioning

confidence: 99%

“…Presumably the diffusion inefficiency can influence the protein extraction yield after PEF treatment, i.e. the concentration difference of proteins from intracellular to extracellular medium impact the yield of PEF-extraction (Scherer et al, 2019). Thus, processing of highly concentrated biomass through PEF treatment for industrial scale needs to be investigated.…”

Section: Introductionmentioning

confidence: 99%

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Impact of incubation conditions on protein and C-Phycocyanin recovery from Arthrospira platensis post- pulsed electric field treatment

Akaberi

Krust

Müller

et al. 2020

Bioresource Technology

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Pulsed electric field (PEF) was conducted for the extraction of proteins/C-Phycocyanins from Arthrospira platensis. The cyanobacterial suspension was treated with 1 µs long pulses at an electric field strength of 40 kV•cm-1 and a treatment energy of 114 kJ•kgsus −1 and 56 kJ•kgsus −1. For benchmarking, additional biomass was processed by high pressure homogenization. Homogeneity of the suspension prior to PEF-treatment influenced the protein/C-phycocyanin extraction efficiency. Stability of C-Phycocyanin during post-PEF incubation time was affected by incubation temperature and pH of the external medium. Biomass concentration severely affect proteins/C-Phycocyanins extraction yield via PEF-treatment. The optimum conditions for extraction of proteins/C-Phycocyanin was obtained at 23 °C while incubating in pH 8-buffer. The energy demand for PEF-treatment amounts to 0.56 MJ•kgdw-1 when processing biomass at 100 gdw•kgsus −1. PEF treatment enhances the C-Phycocyanin purity ratio, thus, it can be suggested as preferential downstream processing method for the production of C-Phycocyanin from A. platensis biomass.

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Section: Effect Of Incubation Temperature On Protein and C-phycocyanimentioning

confidence: 64%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Impact of incubation conditions on protein and C-Phycocyanin recovery from Arthrospira platensis post- pulsed electric field treatment

Akaberi

Krust

Müller

et al. 2020

Bioresource Technology

Self Cite

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“…Nevertheless, new studies also show that the mass transfer is further enhanced by simply incubating the permeabilized cells after the PEF treatment. The associated decrease of the transport resistance is believed to be caused by an enzymatically driven autolysis in response to PEFinduced cell death (Silve et al, 2018a;Jaeschke et al, 2019;Scherer et al, 2019). Therefore, effects on short and on long time scales should be considered for the goal of improving the efficiency of microalgae biorefining by applying PEF technology.…”

Section: Discussion Of Kinetic Modeling and Numerical Simulation As Tmentioning

confidence: 99%

Kinetic Modeling and Numerical Simulation as Tools to Scale Microalgae Cell Membrane Permeabilization by Means of Pulsed Electric Fields (PEF) From Lab to Pilot Plants

Knappert

McHardy

Rauh

2020

Front. Bioeng. Biotechnol.

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Pulsed Electric Fields (PEF) is a promising technology for the gentle and energy efficient disruption of microalgae cells such as Chlorella vulgaris. The technology is based on the exposure of cells to a high voltage electric field, which causes the permeabilization of the cell membrane. Due to the dependency of the effective treatment conditions on the specific design of the treatment chamber, it is difficult to compare data obtained in different chambers or at different scales, e.g., lab or pilot scale. This problem can be overcome by the help of numerical simulation since it enables the accessibility to the local treatment conditions (electric field strength, temperature, flow field) inside a treatment chamber. To date, no kinetic models for the cell membrane permeabilization of microalgae are available what makes it difficult to decide if and in what extent local treatment conditions have an impact on the permeabilization. Therefore, a kinetic model for the perforation of microalgae cells of the species Chlorella vulgaris was developed in the present work. The model describes the fraction of perforated cells as a function of the electric field strength, the temperature and the treatment time by using data which were obtained in a milliliter scale batchwise treatment chamber. Thereafter, the model was implemented in a CFD simulation of a pilot-scale continuous treatment chamber with colinear electrode arrangement. The numerical results were compared to experimental measurements of cell permeabilization in a similar continuous treatment chamber. The predicted values and the experimental data agree reasonably well what demonstrates the validity of the proposed model. Therefore, it can be applied to any possible treatment chamber geometry and can be used as a tool for scaling cell permeabilization of microalgae by means of PEF from lab to pilot scale. The present work provides the first contribution showing the applicability of kinetic modeling and numerical simulation for designing PEF processes for the purpose of biorefining microalgae biomass. This can help to develop new processes and to reduce the costs for the development of new treatment chamber designs.

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Non‐thermal Processing Technologies

2021

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Pulsed electric field (PEF)-assisted protein recovery from Chlorella vulgaris is mediated by an enzymatic process after cell death

Cited by 49 publications

References 31 publications

Impact of incubation conditions on protein and C-Phycocyanin recovery from Arthrospira platensis post- pulsed electric field treatment

Impact of incubation conditions on protein and C-Phycocyanin recovery from Arthrospira platensis post- pulsed electric field treatment

Kinetic Modeling and Numerical Simulation as Tools to Scale Microalgae Cell Membrane Permeabilization by Means of Pulsed Electric Fields (PEF) From Lab to Pilot Plants

Non‐thermal Processing Technologies

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