Impact of incubation conditions on protein and C-Phycocyanin recovery from Arthrospira platensis post- pulsed electric field treatment

Abstract

Pulsed electric field (PEF) was conducted for the extraction of proteins/C-Phycocyanins from Arthrospira platensis. The cyanobacterial suspension was treated with 1 µs long pulses at an electric field strength of 40 kV•cm-1 and a treatment energy of 114 kJ•kgsus −1 and 56 kJ•kgsus −1. For benchmarking, additional biomass was processed by high pressure homogenization. Homogeneity of the suspension prior to PEF-treatment influenced the protein/C-phycocyanin extraction efficiency. Stability of C-Phycocyanin during post-PEF incubation time was affected by incubation temperature and pH of the external medium. Biomass concentration severely affect proteins/C-Phycocyanins extraction yield via PEF-treatment. The optimum conditions for extraction of proteins/C-Phycocyanin was obtained at 23 °C while incubating in pH 8-buffer. The energy demand for PEF-treatment amounts to 0.56 MJ•kgdw-1 when processing biomass at 100 gdw•kgsus −1. PEF treatment enhances the C-Phycocyanin purity ratio, thus, it can be suggested as preferential downstream processing method for the production of C-Phycocyanin from A. platensis biomass.

Assessment of Phycocyanin Extraction from Cyanidium caldarium by Spark Discharges, Compared to Freeze-Thaw Cycles, Sonication, and Pulsed Electric Fields

“…A possible explanation could be that the phycobiliproteins do not only have to cross the cell membrane, but also dissociate from the thylakoid membrane. In this study, no phycocyanin was detected after 120 min incubation time, which is comparable to the results of Martinez et al Additionally, Akaberi et al [ 42 ] found a decrease in phycocyanin release and increase in incubation time (from 1 h to 5 h) when the biomass density was raised from 3.6 g/kg dw to 9.7 g/kg dw. It was concluded that release kinetics at high biomass concentration are significantly slower compared to low applied biomasses.…”

Enhancing Phycoerythrin and Phycocyanin Production from Porphyridium cruentum CCALA 415 in Synthetic Wastewater: The Application of Theoretical Methods on Microalgae

“…Pulsed electric field was used for the production of PC from Arthrospira platensis. 1 µs pulses at an electric field strength of 40 kV•cm −1 were tested and the highest PC concentration was obtained [51]. Nostoc commune TUBT05 and Oscillatoria okeni TISTR8549 was investigated to obtain higher PC production.…”

Neochloris oleoabundans from nature to industry: a comprehensive review

“…Nonetheless, the poor efficacy of PEF to break the cell wall and release intracellular components of rigid cell walled microalgae does not necessarily imply that PEF will not be efficient on weak cell walled species (Safi et al 2017). For instance, by means of PEF, highly pure b-Pycoerythrin and C-Phycocyanin were successfully extracted from Porphyridium cruentum and Arthrospira platensis, respectively (Martínez et al 2019;Akaberi et al 2020).…”