Pulse Generators for Electrofusion and Electroporation

Chassy, Bruce M.; Saunders, James A.; Sowers, Arthur E.

doi:10.1016/b978-0-08-091727-6.50038-1

Cited by 3 publications

(2 citation statements)

References 4 publications

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“…Second, strains with lower survival rates, such as P. cepacia J82R and R. meliloti 1021 ( Fig. 2A and B), tend to have lower transformation frequencies per survivor (e.g., 5 x 10' to 5 X 10-4). For strains with higher rates of survival, such asA.…”

Section: Resultsmentioning

confidence: 99%

Electrical Energy Changes Conductivity and Determines Optimal Electrotransformation Frequency in Gram-Negative Bacteria

Bowen

Kosslak

1992

Appl Environ Microbiol

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In many bacterial electrotransformation protocols, pulse time is related to the time constant for a capacitor discharging across a sample of fixed resistance. Using an electroporator which controls pulse time independently of the capacitor time constant, we found that the resistance of bacterial suspensions fluctuates widely during capacitor discharge. With three gram-negative species of bacteria, electrotransformation frequency and survival could be more simply related to the electrical energy delivered in each pulse than to component parameters, such as initial field strength, capacitance, and pulse time. In each case, the number of transformants per survivor increased exponentially and leveled off when more than 0.5 to 1.0 J of electrical energy was delivered. An inverse log-linear relationship between survival and energy delivered was also observed for all three species.

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Section: Resultsmentioning

confidence: 99%

Electrical Energy Changes Conductivity and Determines Optimal Electrotransformation Frequency in Gram-Negative Bacteria

Bowen

Kosslak

1992

Appl Environ Microbiol

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“…Three to generate electroporation pulses longer than 1 µs and two to generate electroporation pulses shorter than 1 µs. Pulses longer than 1 µs are usually generated by a capacitor discharge, square wave generator or an analog generator [19][20][21][22]. Pulses shorter than 1 µs are usually generated by pulse forming networks or resonant charging generators [23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Advantages and Disadvantages of Different Concepts of Electroporation Pulse Generation

Reberšek

Miklavčič

2011

Automatika

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Review Electroporator is a generator of electric pulses that is used for permeabilization of cells. There are five major concepts of electroporation design. Capacitor discharge, square wave generator, and analog generator are used to generate classical electroporation pulses that are longer than microsecond and pulse forming network, and resonant charging generator that are used to generate nanosecond electroporation pulses. The choice of an electroporator design is always driven by the biotechnological or biomedical application. Electroporators can be used for introduction of small (electrochemotherapy) and large molecules (gene electrotransfer), cell fusion, insertion of proteins into cell membrane, electroporation of organelles, pasteurization, tissue ablation etc. Basic concepts and foreseeable future developments in electroporator design are presented in this article.

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Plant Transformation via Protoplast Electroporation

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Plant Cell Culture Protocols

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Pulse Generators for Electrofusion and Electroporation

Cited by 3 publications

References 4 publications

Electrical Energy Changes Conductivity and Determines Optimal Electrotransformation Frequency in Gram-Negative Bacteria

Electrical Energy Changes Conductivity and Determines Optimal Electrotransformation Frequency in Gram-Negative Bacteria

Advantages and Disadvantages of Different Concepts of Electroporation Pulse Generation

Plant Transformation via Protoplast Electroporation

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