Plant Transformation via Protoplast Electroporation

Bates, George W.

doi:10.1385/1-59259-583-9:359

Cited by 17 publications

(7 citation statements)

References 6 publications

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“…In all of the assays, the renilla luciferase construct was cotransfected to serve as the internal control. Protoplast transfections were performed by electroporation (Saunders et al, 1995;Bates, 1999). After 20 h of incubation, the protoplasts were lysed and used for the dual luciferase assay (Chen et al, 2008;Shi et al, 2011).…”

Section: Transactivation Assaymentioning

confidence: 99%

Rice GROWTH UNDER DROUGHT KINASE Is Required for Drought Tolerance and Grain Yield under Normal and Drought Stress Conditions

et al. 2014

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Rice (Oryza sativa) is the primary food source for more than one-half of the world's population. Because rice cultivation is dependent on water availability, drought during flowering severely affects grain yield. Here, we show that the function of a drought-inducible receptor-like cytoplasmic kinase, named GROWTH UNDER DROUGHT KINASE (GUDK), is required for grain yield under drought and well-watered conditions. Loss-of-function gudk mutant lines exhibit sensitivity to salinity, osmotic stress, and abscisic acid treatment at the seedling stage, and a reduction in photosynthesis and plant biomass under controlled drought stress at the vegetative stage. The gudk mutants interestingly showed a significant reduction in grain yield, both under normal well-watered conditions and under drought stress at the reproductive stage. Phosphoproteome profiling of the mutant followed by in vitro assays identified the transcription factor APETALA2/ETHYLENE RESPONSE FACTOR OsAP37 as a phosphorylation target of GUDK. The involvement of OsAP37 in regulating grain yield under drought through activation of several stress genes was previously shown. Our transactivation assays confirmed that GUDK is required for activation of stress genes by OsAP37. We propose that GUDK mediates drought stress signaling through phosphorylation and activation of OsAP37, resulting in transcriptional activation of stress-regulated genes, which impart tolerance and improve yield under drought. Our study reveals insights around drought stress signaling mediated by receptorlike cytoplasmic kinases, and also identifies a primary regulator of grain yield in rice that offers the opportunity to improve and stabilize rice grain yield under normal and drought stress conditions.

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Section: Transactivation Assaymentioning

confidence: 99%

Rice GROWTH UNDER DROUGHT KINASE Is Required for Drought Tolerance and Grain Yield under Normal and Drought Stress Conditions

et al. 2014

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“…Protoplast transformation comprises separating plant cells that have had their cell walls removed (protoplasts), which are then transformed utilizing genome editing components. Plant tissue may be processed enzymatically to remove the cell walls, producing protoplasts (Bates, 1999). The genome editing components, such as plasmids or ribonucleoprotein complexes (RNPs), are transported into the protoplasts using techniques such as PEGmediated transformation or electroporation.…”

Section: Protoplast Transformationmentioning

confidence: 99%

Genome Editing for Early and Late Flowering in Plants

IRFAN,

HAIDER,

SHAFIQ

et al. 2023

Bull. Biol. All. Sci. Res.

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The use of genome editing to change the blooming period of plants has emerged as a valuable approach in contemporary agricultural research. This chapter delves into the complex processes that control early and late flowering in plants and how genome editing techniques such as CRISPR-Cas9 have altered the field. The chapter begins with an overview of the genetic pathways and regulatory networks that determine flowering time and then dives into the vital functions of key genes such as FLOWERING LOCUS T (FT), CONSTANS (CO), and FLOWERING LOCUS C (FLC). The chapter then delves into the many genome editing methods used to modify blooming time, focusing on augmentation and delay. Researchers have improved agricultural productivity, stress tolerance, and adaptation to changing climatic conditions by targeting regulatory genes. Case studies show effective genome editing applications in various plant species, indicating the possibility of crop development with personalized flowering time alterations. The ethical concerns and potential ecological implications of genome-edited plants with changed flowering times are also discussed, highlighting the significance of responsible research and environmental risk assessment. Furthermore, the chapter investigates the challenges and potential paths in the realm of genome editing for modifying flowering times in plants. This includes a comprehensive review of techniques to achieve more precise genetic modifications, strategies for reducing unintended alterations, and establishing regulatory guidelines.

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“…Plant cell electroporation generally utilizes the protoplast because thick plant cell walls restrict macromolecule movement [10]. Electrical pulses are applied to a suspension of protoplasts with DNA placed between electrodes in an electroporation cuvette.…”

Section: Electroporationmentioning

confidence: 99%

Comparative Review of Plant Transformation Techniques

Okpe

Nkaa

2021

JABB

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Plant transformation is now an important biotechnological tool in plant biology and a practical tool for transgenic plant development. There are many verified methods for stable introduction of novel genes into the nuclear genomes of diverse plant species. As a result, gene transfer and regeneration of transgenic plants are no longer the factors limiting the development and application of practical transformation systems for many plant species. However, the desire for higher transformation efficiency has stimulated work on not only improving various existing methods but also in inventing novel methods. Different methods of transferring the gene into plant cells have been developed and continuous efforts have been made to increase its efficiency. Both direct and indirect methods of gene transfer have their own merits and demerits. Efforts have been made continuously to eliminate drawbacks and to develop an easy and eco-friendly method to transfer foreign genes. Many methods of genetic transformation have been proposed and tried in the laboratories, but most of them result to transient expressions. However, transformation work based on particle bombardment with DNA coated micro projectiles and Agrobacterium mediated transformation have proved to be promising in producing stable transgenic plants from a range of plant species.

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Plant Transformation via Protoplast Electroporation

Cited by 17 publications

References 6 publications

Rice GROWTH UNDER DROUGHT KINASE Is Required for Drought Tolerance and Grain Yield under Normal and Drought Stress Conditions

Rice GROWTH UNDER DROUGHT KINASE Is Required for Drought Tolerance and Grain Yield under Normal and Drought Stress Conditions

Genome Editing for Early and Late Flowering in Plants

Comparative Review of Plant Transformation Techniques

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