Pulse electromagnetic fields enhance the repair of rabbit articular cartilage defects with magnetic nano-hydrogel

Huang, Jianghong; Jia, Zhaofeng; Liang, Yujie; Huang, Zhiwang; Rong, Zhibin; Xiong, Jianyi; Wang, Daping

doi:10.1039/c9ra07874f

Cited by 38 publications

(23 citation statements)

References 33 publications

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“…IONPs physically coated on scaffolds might leak into the surrounding environment, which would weaken the inductive coupling magnetic effect. One solution is to chemically connect the embedded IONPs to the hydrogel so that they are less likely to diffuse into the surrounding environment and be ingested by surrounding cells ( Huang et al., 2020 ).…”

Section: Classification Of Nanocomposite Hydrogelsmentioning

confidence: 99%

RETRACTED: Application of Inorganic Nanocomposite Hydrogels in Bone Tissue Engineering

Han¹,

Xu²,

Che³

et al. 2020

iScience

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Summary Bone defects caused by trauma and surgery are common clinical problems encountered by orthopedic surgeons. Thus, a hard-textured, natural-like biomaterial that enables encapsulated cells to obtain the much-needed biophysical stimulation and produce functional bone tissue is needed. Incorporating nanomaterials into cell-laden hydrogels is a straightforward tactic for producing tissue engineering structures that integrate perfectly with the body and for tailoring the material characteristics of hydrogels without hindering nutrient exchange with the surroundings. In this review, recent developments in inorganic nanocomposite hydrogels for bone tissue engineering that are of vital importance but have not yet been comprehensively reviewed are summarized.

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Section: Classification Of Nanocomposite Hydrogelsmentioning

confidence: 99%

RETRACTED: Application of Inorganic Nanocomposite Hydrogels in Bone Tissue Engineering

Han¹,

Xu²,

Che³

et al. 2020

iScience

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“…In this study, we showed that MF exposure influences the cytoskeletal fiber organization. GAG deposition in magnetically stimulated ADSC-MNP was almost 5 times higher compared to non-loaded WJMSC, pointing to a possible modality for manipulating ADSC for improved chondrogenic conversion in vitro and, possibly, future methods for improving cartilage-repair pulsed magnetic field exposure (PEMF) of BM-ADSC in combination with magnetic hydrogels which were shown to increase repair in rabbit model of cartilage defects ( Huang et al, 2019 ). Further studies are needed to more precisely clarify the molecular mechanism governing the correlation between MNP positioning, MF exposure and increased chondrogenesis in ADSC.…”

Section: Discussionmentioning

confidence: 99%

Magnetic Nanoparticles and Magnetic Field Exposure Enhances Chondrogenesis of Human Adipose Derived Mesenchymal Stem Cells But Not of Wharton Jelly Mesenchymal Stem Cells

Lăbuşcă

Herea

Minuti

et al. 2021

Front. Bioeng. Biotechnol.

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Purpose: Iron oxide based magnetic nanoparticles (MNP) are versatile tools in biology and medicine. Adipose derived mesenchymal stem cells (ADSC) and Wharton Jelly mesenchymal stem cells (WJMSC) are currently tested in different strategies for regenerative regenerative medicine (RM) purposes. Their superiority compared to other mesenchymal stem cell consists in larger availability, and superior proliferative and differentiation potential. Magnetic field (MF) exposure of MNP-loaded ADSC has been proposed as a method to deliver mechanical stimulation for increasing conversion to musculoskeletal lineages. In this study, we investigated comparatively chondrogenic conversion of ADSC-MNP and WJMSC with or without MF exposure in order to identify the most appropriate cell source and differentiation protocol for future cartilage engineering strategies.Methods: Human primary ADSC and WJMSC from various donors were loaded with proprietary uncoated MNP. The in vitro effect on proliferation and cellular senescence (beta galactosidase assay) in long term culture was assessed. In vitro chondrogenic differentiation in pellet culture system, with or without MF exposure, was assessed using pellet histology (Safranin O staining) as well as quantitative evaluation of glycosaminoglycan (GAG) deposition per cell.Results: ADSC-MNP complexes displayed superior proliferative capability and decreased senescence after long term (28 days) culture in vitro compared to non-loaded ADSC and to WJMSC-MNP. Significant increase in chondrogenesis conversion in terms of GAG/cell ratio could be observed in ADSC-MNP. MF exposure increased glycosaminoglycan deposition in MNP-loaded ADSC, but not in WJMSC.Conclusion: ADSC-MNP display decreased cellular senescence and superior chondrogenic capability in vitro compared to non-loaded cells as well as to WJMSC-MNP. MF exposure further increases ADSC-MNP chondrogenesis in ADSC, but not in WJMSC. Loading ADSC with MNP can derive a successful procedure for obtaining improved chondrogenesis in ADSC. Further in vivo studies are needed to confirm the utility of ADSC-MNP complexes for cartilage engineering.

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“…In another study, carboxylated cellulose nanocrystals (cCNCs) were prepared using ammonium persulfate as hydrogel inks, and stable cell-free and cell-loaded hydrogel inks with the best physicochemical properties and biocompatibility were developed [ 7 ]. We also used magnetic nanoparticles (Fe 2 O 3 ) as a bioink to generation magnetic nanocomposite hydrogel for cartilage tissue engineering [ 8 , 9 , 10 , 11 , 12 ].…”

Section: Bioinks For 3d Bioprintingmentioning

confidence: 99%

3D Bioprinting of Hydrogels for Cartilage Tissue Engineering

Huang

Xiong

Wang

et al. 2021

Gels

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Three-dimensional (3D) bioprinting is an emerging technology based on 3D digital imaging technology and multi-level continuous printing. The precise positioning of biological materials, seed cells, and biological factors, known as “additive biomanufacturing”, can provide personalized therapy strategies in regenerative medicine. Over the last two decades, 3D bioprinting hydrogels have significantly advanced the field of cartilage and bone tissue engineering. This article reviews the development of 3D bioprinting and its application in cartilage tissue engineering, followed by a discussion of the current challenges and prospects for 3D bioprinting. This review presents foundational information on the future optimization of the design and manufacturing process of 3D additive biomanufacturing.

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Pulse electromagnetic fields enhance the repair of rabbit articular cartilage defects with magnetic nano-hydrogel

Abstract: Pulsed electromagnetic fields combined with magnetic nano-hydrogel can promote bone marrow mesenchymal stem cells to repair rabbit articular cartilage defects.

Cited by 38 publications

References 33 publications

RETRACTED: Application of Inorganic Nanocomposite Hydrogels in Bone Tissue Engineering

RETRACTED: Application of Inorganic Nanocomposite Hydrogels in Bone Tissue Engineering

Magnetic Nanoparticles and Magnetic Field Exposure Enhances Chondrogenesis of Human Adipose Derived Mesenchymal Stem Cells But Not of Wharton Jelly Mesenchymal Stem Cells

3D Bioprinting of Hydrogels for Cartilage Tissue Engineering

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