Pulse-Amplitude-Modulation (PAM) Fluorometry and Saturation Pulse Method: An Overview

Schreiber, Ulrich

doi:10.1007/978-1-4020-3218-9_11

Cited by 706 publications

(562 citation statements)

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“…Photosystem II photochemical efficiency: pulse amplitude modulation fluorometry Samples were evaluated for maximum quantum yield of the Photosystem II (Fv/Fm) using a submersible pulse-amplitude-modulated chlorophyll fluorometer (Diving-PAM, Walz, Effeltrich, Germany) as a proxy for the 'health' of the coral holobiont (Schreiber, 2004). The measurements were taken after sunset in order to avoid interference by diurnal photo-inhibition artifacts, after full recovery of the reaction centers.…”

Section: Methodsmentioning

confidence: 99%

Climate change affects key nitrogen-fixing bacterial populations on coral reefs

Santos

Carmo

Duarte³

et al. 2014

The ISME Journal

121

111

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Coral reefs are at serious risk due to events associated with global climate change. Elevated ocean temperatures have unpredictable consequences for the ocean's biogeochemical cycles. The nitrogen cycle is driven by complex microbial transformations, including nitrogen fixation. This study investigated the effects of increased seawater temperature on bacteria able to fix nitrogen (diazotrophs) that live in association with the mussid coral Mussismilia harttii. Consistent increases in diazotroph abundances and diversities were found at increased temperatures. Moreover, gradual shifts in the dominance of particular diazotroph populations occurred as temperature increased, indicating a potential future scenario of climate change. The temperature-sensitive diazotrophs may provide useful bioindicators of the effects of thermal stress on coral reef health, allowing the impact of thermal anomalies to be monitored. In addition, our findings support the development of research on different strategies to improve the fitness of corals during events of thermal stress, such as augmentation with specific diazotrophs.

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Section: Methodsmentioning

confidence: 99%

Climate change affects key nitrogen-fixing bacterial populations on coral reefs

Santos

Carmo

Duarte³

et al. 2014

The ISME Journal

121

111

View full text Add to dashboard Cite

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“…Measurements of PRI in the canopy of leaves (from single plant or group of plants) can be also based on the application of spectrometers or multispectral and hyperspectral cameras [15,61], which can be placed on a mobile platform (e.g., drone) or fixed at a certain distance from the canopy [35,38]. Photosynthetic parameters in leaves (in particular, ∆F/Fm', NPQ, and LUE) can be measured by standard methods, including PAM-fluorometry [16,17] and analysis of CO 2 exchange [21][22][23][24][25]. However, the application of these methods on the canopy level is a very difficult problem.…”

Section: Main Principles Of Data Analysismentioning

confidence: 99%

“…As a result, remote sensing of the photosynthetic process is an important practical problem [13][14][15]. There are many methods that can be used for the analysis of the photosynthesis process in plants; in particular, pulse-amplitude-modulation (PAM)-fluorometry [16,17], JIP-test [18][19][20], and analysis of CO 2 exchange [21][22][23][24][25]. These methods are very effective in the laboratory.…”

Section: Introductionmentioning

confidence: 99%

Connection of the Photochemical Reflectance Index (PRI) with the Photosystem II Quantum Yield and Nonphotochemical Quenching Can Be Dependent on Variations of Photosynthetic Parameters among Investigated Plants: A Meta-Analysis

Sukhova

Sukhov

2018

Remote Sensing

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Abstract:The development of spectral methods of remote sensing, including measurement of a photochemical reflectance index (PRI), is a prospective trend in precision agriculture. There are many works which have investigated the connection between photosynthetic parameters and PRI; however, their results varied and were sometimes contradictory. For this paper, we performed a meta-analysis of works in this field. Here, only linear correlations of PRI with photosynthetic parameters-including quantum yield of photosystem II (∆F/Fm'), nonphotochemical quenching of chlorophyll fluorescence (NPQ), and light use efficiency (LUE)-were investigated. First, it was shown that the correlations were dependent on conditions of PRI measurements (leaf or canopy; artificial light or sunlight). Second, it was shown that a minimal level of the photosynthetic stress, and the variation of this level among investigated plants, can influence the linear correlation of PRI with ∆F/Fm' and NPQ; the effect was dependent on conditions of measurements. In contrast, the distribution of LUE among plants did not influence its correlation with PRI. Thus, the meta-analysis shows that the distribution of photosynthetic parameters among investigated plants can be an important factor that influences the efficiency of remote sensing on the basis of the PRI measurement.

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“…Pulse amplitude modulation (PAM) fluorometry has produced a large number of fluorescence investigations and practical applications (Schreiber 2004). Minimum fluorescence (F o ) is measured with a low intensity far-red light (≈6 nmol m −2 s −1 at 660 nm), while the maximum fluorescence (F m ) is measured using a high intensity white light saturation pulse (≈10,000 μmol m −2 s −1 at 400-700 nm).…”

Section: Introductionmentioning

confidence: 99%