Pulmonary metastases in guinea pigs as a consequence of dermal implantation of line-10 tumor cells

Treatment by limited surgery and specific immunization of guinea pigs with stage II experimental cancer.

“…Inoculation of 10 6 L10 tumor cells intradermally in the flank 2 cm posterior to the axillae of animals that are left untreated leads to progressive intradermal tumor growth, metastasis of tumor cells to draining lymph nodes, and death. At autopsy, 30-40% of the animals are found to have gross, visible pulmonary metastases (3). Limited surgery designed to remove the dermal tumor and to leave microscopic lymph node metastases in place is not curative, but prolongs survival of the guinea pigs ; at autopsy, the majority of them are found to have pulmonary as well as lymph node metastases (3).…”

“…If a systemic specific immune response to the tumor could be generated by active immunotherapy, malignant cells not removed at surgery might be eliminated. Several animal models have been used to demonstrate the efficacy of immunization with tumor cell vaccines in the treatment of animals with subcutaneous, intradermal, or intravenous transplants of live tumor cells (3,(7)(8)(9)(10) . Living bacillus Calmette-Guerin (BCG) as well as BCG CW on oil droplets have been shown to be effective adjuvants in generating systemic immunity to L10 (2,6,7,11) .…”

“…A majority of guinea pigs, each with a growing syngeneic tumor implant in the skin and no other outward sign of malignancy (clinical stage I disease) but with tumor cells in the regional lymph nodes, was cured by tumor-specific immunization after limited surgery consisting of excision of the dermal tumor (1, 2). Animals treated by limited surgery alone were not cured; they developed palpable tumors in the draining lymph nodes, and at autopsy, 70-80% of the animals were found to have pulmonary metastases (3) . We now report the results of studies to test the efficacy of limited surgery and tumor-specific immunization in the treatment of animals, each with a dermal tumor and a palpable draining lymph node (clinical stage II disease) .…”

Anti-tumour activity of Lactobacillus casei on lewis lung carcinoma and line-10 hepatoma in syngeneic mice and guinea pigs

“…injection of living BCG into established line-10 hepatoma caused turnout regression and induced systemic and specific tumour immunity in syngeneic strain-2 guinea pigs. Since then, the line-10 hepatoma model has been used in studies of specific and non-specific immunotherapy of cancer [7,13,14,21,25,26,29,35,36]. These 3LL and line-10 tumour models have provided an opportunity for the study of immunotherapy in experimental systems analogous to human tumour metastasis.…”