Changing tumour antigen expression in metastatic hepatocellular carcinoma cells of the guinea pig

Molengraft, Fred van de; Poels, L. G.; Niekerk, Catharina C. van; Mungyer, Gertrude; Steerenberg, P. A.; Jap, P. H. K.

doi:10.1007/bf02890020

Cited by 4 publications

(1 citation statement)

References 24 publications

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“…mAb to line-10 antigens 10TL1, 10TL40 and 10TL43 [29,42] were diluted 1 : 500. mAb to guinea pig MHC class II antigens 25E3 and 27E7 were kindly provided by Dr. Shevach [6] (National Institute of Allergy and Infectious Diseases, Bethesda, Md., USA) and diluted 1 : 500. mAb OKIa to human MHC class II antigens were obtained from Ortho Diagnostics Systems (Beerse, Belgium) and diluted l : 80. MSgp4, reacting with MHC class I antigens from guinea pigs was kindly provided by Dr Baker [20] (Hunterian Institute, Royal College of Surgeons of England, London, UK).…”

Section: Monoclonal Antibodiesmentioning

confidence: 99%

Major histocompatibility complex class II antigen expression during potentiation of line-10 tumor immunity after intralesional administration of bacillus Calmette-Guérin

Steerenberg

Jong

Geerse

et al. 1990

Cancer Immunol Immunother

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Intralesional injection of BCG into an established line-10 hepatocellular carcinoma in the strain-2 guinea pig causes regression of the tumor and induction of line-10 immunity. We found that the animals were already protected for a second challenge with line-10 tumor cells 7 days after BCG treatment. We studied whether this early induction of immunity was correlated with the expression of MHC class II antigens on line-10 tumor cells and was correlated with an increased expression of MHC class II antigens on leukocytes in the primary tumor and in the regional lymph node (Ln. axillaris accessorius). The MHC class II antigens and the leukocyte subpopulations were measured with monoclonal antibodies and flow cytofluorometry. In the draining lymph node the number of nucleated cells increased about 10-fold during the first 5 days after intralesional injection of BCG. At this time the MHC class II antigen expression of these cells was increased from 21%-32% in the naive controls to 39%-53% in animals with BCG-treated tumors. This implies that the number of MHC-class-II-positive cells increased about 20-fold in the draining lymph node. Surprisingly, the increase in percentage of MHC-class-II-antigen-positive cells was mainly due to an increase of IgM-positive B cells from 8%-11% to 22%-41% and an increase of IgG-positive B cells from 7%-27% to 25%-44%. In the tumor, BCG treatment induced a small increase of MHC-class-II-antigen-positive cells from 11%-12% to 15%-20%. Probably this increase came not from tumor cells but mainly from a BCG-induced infiltration of mononuclear cells, as an increase of T cells from 14% to 20%, an increase of macrophages from 8% to 18%, and an increase of B cells from 0 to 6% was observed. We conclude that the potentiation of anti-(line-10 tumor cell) immunity correlated with a 20-fold increase of MHC-class-II-antigen-positive cells in the lymph nodes and a small increase in the number of MHC-class-II-antigen-positive tumor-infiltrating cells.

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Section: Monoclonal Antibodiesmentioning

confidence: 99%

Major histocompatibility complex class II antigen expression during potentiation of line-10 tumor immunity after intralesional administration of bacillus Calmette-Guérin

Steerenberg

Jong

Geerse

et al. 1990

Cancer Immunol Immunother

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Immunohistochemical demonstration of keratin 7 in routinely fixed paraffin‐embedded human tissues

Niekerk

Jap

Ramaekers

et al. 1991

The Journal of Pathology

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The immunoreactivity of OV-TL 12/30, a monoclonal anti-keratin 7 antibody (Mab), was investigated on frozen as well as paraffin-embedded human tissues. Its reactivity patterns were compared with another well-characterized monoclonal antibody to keratin 7 (RCK 105), and with broadly cross-reacting monoclonal (OV-TL 12/5) as well as polyclonal (pKer) keratin antisera. In frozen sections of normal and malignant human tissues both keratin 7 Mabs gave similar staining patterns. The immunoreactivity for OV-TL 12/30 and the polyclonal antibody (pKer) in tissue sections fixed in 4 per cent formalin or Bouin solution, was completely restored when pretreated with 0.1 per cent pronase, 0.1 per cent trypsin in phosphate-buffered saline (PBS) or with 0.5 per cent pepsin in 0.01 N HCl. Except for loss of immunoreactivity on human normal stomach surface epithelium and glandular mucous cells, Mab OV-TL 12/30 reacted strongly positive with essentially all those formalin- or Bouin-fixed paraffin-embedded tissues that had been shown to stain in non-fixed, frozen sections. In addition to the good correlation in human tissues, a complete correlation between the reactivity on frozen and paraffin-embedded human carcinomas (n = 86) was found as well. While both RCK 105 (anti-keratin 7) and OV-TL 12/5 (anti-keratin 5, 7, 14, 19) did not stain on paraffin-embedded sections, the polyclonal control antiserum (pKer) lost immunoreactivity in some cell types (e.g. mucous cells in compound glands, hepatocytes, pancreatic acinar cells, and proximal and distal convoluted tubules of the kidney). Our study shows that the keratin 7 Mab OV-TL 12/30 is an excellent marker for tumour histopathology since it is reactive in paraffin-embedded formalin-fixed human tissues.

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Tumor infiltrating leukocytes (tils) during progressive tumor growth and BCG-mediated tumor regression

Steerenberg

Jong

Elgersma

et al. 1990

Virchows Archiv B Cell Pathol

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Changing tumour antigen expression in metastatic hepatocellular carcinoma cells of the guinea pig

Cited by 4 publications

References 24 publications

Major histocompatibility complex class II antigen expression during potentiation of line-10 tumor immunity after intralesional administration of bacillus Calmette-Guérin

Major histocompatibility complex class II antigen expression during potentiation of line-10 tumor immunity after intralesional administration of bacillus Calmette-Guérin

Immunohistochemical demonstration of keratin 7 in routinely fixed paraffin‐embedded human tissues

Tumor infiltrating leukocytes (tils) during progressive tumor growth and BCG-mediated tumor regression

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