Pulmonary injury at the anhepatic phase without veno-venous bypass in portal hypertensive rats

Abstract

Ischemia-reperfusion injury is an important mechanism that results in pulmonary injury after liver transplantation. It is safe for portal hypertensive rats to tolerate 1 hour at the anhepatic phase. Pulmonary injury was the most severe within 12-24 hours after ischemia-reperfusion.

Characterization and evolution of intestine injury at the anhepatic phase in portal hypertensive rats

“…The use of this model is suitable to evaluate the influence of anhepatic hypotension and inferior vena cava congestion on small intestine with portal vein shunt. In the previous experiments with the model, it may be difficult to locate the portal vein, which persisted following cutting the narrow line (17,19). After constructing the model of portal hypertension secondary to liver cirrhosis, the portal vein could not be completely restored.…”

“…However, the portal venous resistance will recover after clinical liver transplantation. To rectify this issue, the surgical procedure based on previous procedures (17,19) was modified. The 20-gauge blunt needle, self-made label tube and portal vein were ligated together, and then the needle was drawn to make the diameter of portal vein equal to the diameter of the 20-gauge needle.…”

“…Establishment of portal hypertension rat model. Portal hypertension rat models were established as previously described (17,19), with modifications. The 20-gauge blunt needle, self-made label tube and portal vein were ligated together, and then the needle was drawn to make the diameter of portal vein equal to the diameter of 20-gauge needle.…”

“…Intestinal injury following liver transplantation may be caused by many risk factors, such as ischemia and hypoxia, endotoxemia, immune barrier damage, severe infection, hypercoagulability and I/R injury (13)(14)(15). I/R injury has been identified as the most notable reason, which also results in lung and kidney injury (16,17). Recently, researchers have focused on clinical intervention and mechanism of intestinal injury, whereas histopathology analysis in intestinal injury has received less attention (18).…”