Characterization and evolution of intestine injury at the anhepatic phase in portal hypertensive rats

Ren, Guijun; Yuan, Xiaoye; Zhao, Xinyang; Hao, Qingchun; Cao, Jinglin; Wang, Yang; Gao, Qingjun; Dou, Jianmin; Zeng, Qiang

doi:10.3892/etm.2018.6800

Cited by 4 publications

(4 citation statements)

References 36 publications

(38 reference statements)

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“…Sourbrane et al [23] cited splanchnic congestion in cases of prolonged portal venous clamp time or early vein thrombosis as risk factors for gastrointestinal perforation after pediatric LDLT for patients with BA. Furthermore, an experimental animal report indicated progressive damage to the intestinal tract during the anhepatic period [25]. In the present study, the anhepatic period also tended to be longer in cases of gastrointestinal perforation than in those without it.…”

Section: Discussionsupporting

confidence: 60%

The advantages of duct-to-duct biliary reconstruction in pediatric living donor liver transplantation

Toriigahara,

Matsuura,

Yanagi

et al. 2023

Pediatr Surg Int

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Background/Purpose: Whether Roux-en-Y hepatic jejunectomy (HJ) or duct-to-duct biliary reconstruction (DD) is more useful in pediatric living donor liver transplantation has not yet been fully investigated. Therefore, to assess the feasibility and safety of DD, we compared the surgical outcomes of DD to HJ. Methods:We divided 45 patients, excluding those with biliary atresia, into the DD group (n=20) and the HJ group (n=25), according to the type of biliary reconstruction they received. Results:The 5-year survival rates (DD vs. HJ=79.7% vs. 83.6%, p=0.70) and the incidence of biliary complications, including bile leakage and stricture (DD vs. HJ=1 [5.0%] vs. 1 [4.0%], p=0.87) were not signi cantly different between the groups. However, intestinal complications, including bowel perforation or ileus, were signi cantly common in the HJ group (9/25 [36.0%]) than in the DD group (1/20 [5.0%]; p=0.01). The three patients in the HJ group with intestinal perforation all suffered perforation at the anastomosed site in the Roux-en-Y procedure. The subgroup analysis showed the non-inferiority of DD to HJ for biliary or intestinal complications in patients weighting <10 kg. Conclusion:With a proper selection of cases, DD should be a safe method for biliary reconstruction in pediatric recipients with little risk of biliary complications equivalent to HJ and a reduced risk of intestinal complications.

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Section: Discussionsupporting

confidence: 60%

The advantages of duct-to-duct biliary reconstruction in pediatric living donor liver transplantation

Toriigahara,

Matsuura,

Yanagi

et al. 2023

Pediatr Surg Int

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“…Previous studies have verified that the intestinal mucosal barrier damage caused by I/R injury is mainly attributable to low perfusion pressure, oxygen-free radical injuries and the action of cytokines (41,42). While free radicals are constantly produced, there are also certain enzymes that decompose them in an organism (43).…”

Section: Discussionmentioning

confidence: 97%

Remote ischemic post‑conditioning alleviates ischemia/reperfusion‑induced intestinal injury via the ERK signaling pathway‑mediated RAGE/HMGB axis

et al. 2021

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Intestinal ischemia reperfusion (I/R) injury is a tissue and organ injury that frequently occurs during surgery and significantly contributes to the pathological processes of severe infection, injury, shock, cardiopulmonary insufficiency and other diseases. However, the mechanism of intestinal I/R injury remains to be elucidated. A mouse model of intestinal I/R injury was successfully established and the model mice were treated with remote ischemic post-conditioning (RIPOC) and/or an ERK inhibitor (CC-90003), respectively. Histopathological changes of the intestinal mucosa were determined by hematoxylin and eosin staining. In addition, the levels of high-mobility group box 1 (HMGB1) and receptor for advanced glycation end products (RAGE) expression were confirmed by reverse transcription-quantitative polymerase chain reaction, western blotting and immunohistochemistry assays. The levels of antioxidants, oxidative stress markers (8-OHdG) and interleukin 1 family members were evaluated by ELISA assays and the levels of NF-κB pathway proteins were analyzed by western blotting. The data demonstrated that RIPOC could attenuate the histopathological features of intestinal mucosa in the intestinal I/R-injury mouse models via the ERK pathway. It was also revealed that HMGB1 and RAGE expression in the mouse models could be markedly reduced by RIPOC (P<0.05) and that these reductions were associated with inhibition of the ERK pathway. Furthermore, it was demonstrated that RIPOC produced significant antioxidant and anti-inflammatory effects following an intestinal I/R injury and that these effects were mediated via the ERK pathway (P<0.05). In addition, RIPOC was demonstrated to suppress the NF-κB (p65)/NLR family pyrin domain containing 3 (NLRP3) inflammatory pathways in the intestinal I/R injury mouse models via the ERK pathway. The findings of the present study demonstrated that RIPOC helped to protect mice with an intestinal I/R injury by downregulating the ERK pathway.

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“…Rats were sedated with isoflurane, and midline laparotomy was carried out to visualize the portal vein. A 22‐G Elastar needle was inserted into the portal trunk, and the portal pressure was measured using an eight‐channel polygraph (RM‐6000; Nihon Kohden), as previously described 22 …”

Section: Methodsmentioning

confidence: 99%

“…A 22-G Elastar needle was inserted into the portal trunk, and the portal pressure was measured using an eight-channel polygraph (RM-6000; Nihon Kohden), as previously described. 22 Glucose tolerance test Intravenous glucose tolerance tests were undertaken after 12 h of fasting in 18 weeks rats. Blood glucose concentrations were measured using a blood glucose test meter (Antisense III; HORIBA Medical) at 0, 15, 30, and 60 min after intravenous glucose injection (1.5 mg/g body weight).…”

Section: Portal Pressure Measurementmentioning

confidence: 99%

Pancreas stiffness in liver cirrhosis is an indicator of insulin secretion caused by portal hypertension and pancreatic congestion

Imamura

Kumagi

Kuroda

et al. 2021

Hepatology Research

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Aim: Portal hypertension induces pancreatic congestion and impaired insulin secretion in patients with liver cirrhosis (LC). However, its mechanism is unclear, with no established noninvasive imaging method for the evaluation of its pathogeneses. The present study focused on pancreas stiffness, as assessed by shear wave elastography (SWE), and examined its association with portal hypertension and insulin secretion.Methods: Shear wave elastography and contrast-enhanced ultrasonography were utilized to evaluate pancreas stiffness and congestion, respectively. A glucagon challenge test was used for insulin secretion assessment. Furthermore, rat models of carbon tetrachloride (CCl 4 )-induced LC and portal hypertension were used to identify the direct effects of pancreatic congestion. Immunohistochemistry staining of the pancreas was carried out on human autopsy samples.Results: Pancreas stiffness measured by SWE was higher in patients with LC than in controls and showed significant correlation with pancreatic congestion. The glucagon challenge test indicated a lower value for the change in C-peptide immunoreactivity in the LC group, which was inversely correlated with pancreas stiffness and congestion. Additionally, portal hypertension and insulin secretion dysfunction were confirmed in CCl 4 rat models. Autopsy of human samples revealed congestive and fibrotic changes in the pancreas and the relationship between insulin secretion and their factors in patients with LC. Conclusions:In patients with LC, pancreas stiffness measured by SWE could be a potential noninvasive test for evaluating pancreatic congestion and fibrosis due to portal hypertension. Moreover, it was associated with impaired insulin secretion, and could aid in guiding the treatment for hepatogenous diabetes.

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Characterization and evolution of intestine injury at the anhepatic phase in portal hypertensive rats

Cited by 4 publications

References 36 publications

The advantages of duct-to-duct biliary reconstruction in pediatric living donor liver transplantation

The advantages of duct-to-duct biliary reconstruction in pediatric living donor liver transplantation

Remote ischemic post‑conditioning alleviates ischemia/reperfusion‑induced intestinal injury via the ERK signaling pathway‑mediated RAGE/HMGB axis

Pancreas stiffness in liver cirrhosis is an indicator of insulin secretion caused by portal hypertension and pancreatic congestion

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