Pulmonary Granuloma Caused byPseudomonas andersoniisp nov

Abstract

Pulmonary granuloma is a common lesion for which gram-negative bacteria are rarely implicated as a cause. Hence, most physicians are unaware of this etiology. We isolated a gram-negative bacterium from a surgically resected pulmonary granuloma in a 42-year-old, nonimmunocompromised woman. Within the necrotizing granuloma, numerous organisms also were demonstrated by Gram stain, suggesting a cause-disease relationship. Characterization of the bacterium by sequence analysis of the 16S ribosomal gene, cellular fatty acid profiling, and microbiologic studies revealed a novel bacterium with a close relationship to Pseudomonas. We propose a new species for the bacterium, Pseudomonas andersonii. These results suggest that the differential diagnosis of a lung granuloma also should include this gram-negative bacterium as a potential causative agent, in addition to the more common infections caused by acid-fast bacilli and fungi. This bacterium was shown to be susceptible to most antibiotics that are active against gram-negative bacteria.

Isolation and Characterization of “Pseudomonas andersonii” from Four Cases of Pulmonary Granulomas and Emended Species Description

“…Among Ͼ20,000 other 16S rRNA sequencing identifications performed at ARUP, P. andersonii has not been identified from specimens other than lung granulomas, suggesting it has a specific clinical niche. In the original case of P. andersonii, the pathogenic role of the bacterium was evident, as it was found to be intimately involved with the necrotic granulomatous lesion and no other organisms could be isolated or demonstrated (6). In the present cases the pathogenic role of P. andersonii is unclear because either a fungus or M. avium complex was also demonstrated or isolated in each case and all patients recovered without the use of antibiotics.…”

“…In 2001, a novel species, "Pseudomonas andersonii," was implicated as the cause of a pulmonary granuloma (6). Nearly a decade later, this remains the only reported case, and the species name has yet to be validated (4).…”

Chromobacterium haemolyticum sp. nov., a strongly haemolytic species

“…The almost full length 16S rRNA gene of MDA0585 T was amplified by PCR and sequenced according to previously published procedures (Han et al, 2001(Han et al, , 2002. A conserved head portion of the DNA gyrase A gene (gyrA) was amplified and sequenced using primers 59-TACGCGATGAG-CGTGATCGTC and 59-GTTGTGCGGCGGGATGTTGGT (Han & Andrade, 2005).…”

First Reported Infections Caused by Three Newly Described Genera in the Family Xanthomonadaceae

“…The family Xanthomonadaceae was proposed in 2005 to contain 12 genera, including Stenotrophomonas, Xanthomonas, Glucose fermentation Definitive identification of all organisms was reached through amplification of a central segment of the 16S rRNA gene (ϳ590 bp) by using a set of universal primers (forward primer, 5Ј-TGCAGCCGCGGTAATAC-3Ј; reverse primer, 5Ј-CGCT CGTTGCGGGACTTAACC-3Ј) (7). For the two organisms without full matches, nearly full-length segments of the gene were obtained through the use of additional primers (5Ј-GCG TGCTTAACACATGCAAGTC-3Ј and 5Ј-AGGAGGTGATC CAACCGCA-3Ј) (7,8).…”

“…For the two organisms without full matches, nearly full-length segments of the gene were obtained through the use of additional primers (5Ј-GCG TGCTTAACACATGCAAGTC-3Ј and 5Ј-AGGAGGTGATC CAACCGCA-3Ј) (7,8).…”