Puerarin facilitates osteogenesis in steroid-induced necrosis of rabbit femoral head and osteogenesis of steroid-induced osteocytes via miR-34a upregulation

Jiang, Xin; Chen, Wenjing; Su, Hang; Shen, Fuguo; Xiao, Wenlong; Sun, Wencai

doi:10.1016/j.cyto.2021.155512

Cited by 12 publications

(8 citation statements)

References 33 publications

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“…It is clear that our experimental results cannot support the bioinformatics conclusions in this study. However, many previous studies have confirmed that glucocorticoids may induce femoral head necrosis through downregulation of Runx2 [40]. Because our findings from validation experiments were consistent with those from previous studies, we suggest that the database of peripheral blood samples selected for bioinformatics analysis of hormonal osteonecrosis of the femoral head may be more limited.…”

Section: Discussionsupporting

confidence: 88%

A network pharmacology approach and validation experiments to investigate the mechanism of the Chinese medicine Wen-Dan Decoction in the treatment of steroid-induced femoral head necrosis

Li²,

Wu³

et al. 2022

Preprint

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Objective: The present work studied the mechanism of action of Wen-Dan Decoction in treating steroid-induced necrosis of the femoral head (SINFH) via network pharmacology and in vivo experiments.Methods: The microarray dataset GSE123568 (peripheral blood), which includes 30 SINFH patients and 10 non-SINFH samples (after using steroids), is included in the Gene Expression Omnibus (GEO) database. Differential expression analysis and weighted gene coexpression network analysis (WGCNA) were used to identify key SINFH-related genes. To investigate the functions of key SINFH-related genes, Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment analyses were performed. We constructed a protein–protein interaction (PPI) network based on the STRING database. In addition, we screened active components in Wen-Dan Decoction and their possible role in treating hormonal osteonecrosis using the Traditional Chinese Medicine System Pharmacology (TCMSP) Database. Subsequently, the potential mechanisms of action of Wen-Dan Decoction in the treatment of SINFH obtained via network pharmacology analyses were validated in in vivo experiments.Results: A total of 608 DEGs were obtained (230 upregulated, 378 downregulated). In GO functional enrichment analysis, SINFH-related genes were mainly involved in neutrophil activation and immune response, and KEGG pathway analysis revealed that SINFH genes were mainly associated with cytokine receptor interactions, lipids and atherosclerosis, and tuberculosis. In addition, a total of 147 active ingredients were obtained for Wen-Dan Decoction, of which the core ingredient was quercetin, an active ingredient in licorice. Meanwhile, 277 target genes were identified for the treatment of SINFH with Wen-Dan Decoction, and NCF1, PTGS2, and RUNX2 were selected as core target genes. Furthermore, the results of quantitative real-time polymerase chain reaction (qRT–PCR) on peripheral blood and bone tissue samples from SINFH patients and non-SINFH samples showed that PGTS2 and NCF1 were upregulated in patient blood and necrotic bone tissues. In quercetin-treated mice, the appearance of femoral head necrosis was significantly improved, and micro-CT suggested that the bone density was significantly better. Finally, the results of qRT–PCR analysis of peripheral blood and femoral bone tissue from a mouse model of SINFH treated with quercetin showed that PGTS2 and NCF1 levels were downregulated compared with those of controls, which was consistent with the results of bioinformatics analysis.Conclusion: This study provides new insights into the genes and related pathways involved in SINFH and shows that PTGS2 and NCF1 could be used as potential drug targets in SINFH.

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Section: Discussionsupporting

confidence: 88%

A network pharmacology approach and validation experiments to investigate the mechanism of the Chinese medicine Wen-Dan Decoction in the treatment of steroid-induced femoral head necrosis

Li²,

Wu³

et al. 2022

Preprint

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“… 36 Puerarin facilitated osteogenesis of steroid-induced necrosis of rabbit femoral head and osteogenesis of steroid-induced osteocytes via miR-34a upregulation. 37 In our study, Puerarin exhibited protective effects by reducing DNA damage and apoptosis of HUVECs caused by radiation. On the molecular level, Puerarin reduced the expression of miRNA-34a in HUVECs and up-regulated its target PLGF.…”

Section: Discussionsupporting

confidence: 50%

Puerarin Reduces Radiation-Induced Vascular Endothelial Cell Damage Via miR-34a/Placental Growth Factor

Liu

Zhao

et al. 2022

Dose-Response

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The aim is to explore the protective effects of Puerarin on radiation-induced vascular endothelial cell damage and its underlying mechanism. The apoptosis and DNA damage of Human umbilical vascular endothelial cells (HUVECs) exposed to radiation alone or in combination with glucose in the exposed group were significantly elevated ( P < .05) compared with those in the control group. The Puerarin-treated HUVECs showed significant reduction in the radiation-induced apoptosis and DNA damage ( P < .05). Furthermore, X-ray irradiation significantly increased the expression of miR-34a, which was reversed by pre-treatment with Puerarin. Placental Growth Factor (PLGF) was a target gene of miR-34a. The expression of PLGF in the peripheral blood of patients receiving radiotherapy significantly increased with an increase in the cumulative dose of radiation ( P < .05), after which it began to decrease at the fourth week ( P < .05) and then remained at a low level until the end of radiotherapy. Puerarin exerts a radioprotective effect by decreasing DNA damage and apoptosis through miR-34a-targeted PLGF.

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“…In recent years, traditional Chinese medicine (TCM) has played an important role in the treatment of orthopedic diseases [11–13] . There have been increasing numbers of clinical studies on SONFH prevention and treatment with TCM, and the efficacy is significant [14–16] . It has been found clinically that TCM can achieve good results in SONFH treatment, and the results of a large number of animal experimental studies have shown that enhanced TCM use has good effects in SONFH treatment [17,18] …”

Section: Introductionmentioning

confidence: 99%

Effectiveness and safety of traditional Chinese medicine in the treatment of steroid-osteonecrosis of femoral head

He¹,

Chen²,

Chen

et al. 2021

Medicine

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Background:Osteonecrosis of the femoral head (ONFH) is a common refractory disease in orthopedics. Overdose glucocorticoid application is a common trigger for ONFH. Traditional Chinese medicine (TCM), as a treatment for ONFH, has been shown to be effective in treating steroid-induced ONFH (SONFH). However, a systematic review and meta-analysis of them is lacking. We aim to systematically review the effectiveness and safety of TCM in the treatment of SONFH.Methods:We will search the following databases: PubMed, Embase, the Cochrane Library, MEDLINE, the Chinese Biomedical Literature Database, China Science and Technology Journal Database, China National Knowledge Infrastructure, and Wanfang Data (since the inception of the databases to the present). In addition, we will look for clinical trial registrations, prospective grey literature, relevant conference papers, and established study reference lists. We will use Review Manager 5.3 software for meta-analysis and heterogeneity assessment. We will evaluate the quality of the evidence using a hierarchy of recommendation assessment, development, and evaluation.Results:This study will systematically evaluate the efficacy and safety of TCM in the treatment of SONFH.Conclusion:This systematic review to evaluate the effectiveness and safety of TCM in the treatment of SONFH will provide updated evidence for clinical application.INPLASY registration number:INPLASY202170015.

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Puerarin facilitates osteogenesis in steroid-induced necrosis of rabbit femoral head and osteogenesis of steroid-induced osteocytes via miR-34a upregulation

Cited by 12 publications

References 33 publications

A network pharmacology approach and validation experiments to investigate the mechanism of the Chinese medicine Wen-Dan Decoction in the treatment of steroid-induced femoral head necrosis

A network pharmacology approach and validation experiments to investigate the mechanism of the Chinese medicine Wen-Dan Decoction in the treatment of steroid-induced femoral head necrosis

Puerarin Reduces Radiation-Induced Vascular Endothelial Cell Damage Via miR-34a/Placental Growth Factor

Effectiveness and safety of traditional Chinese medicine in the treatment of steroid-osteonecrosis of femoral head

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