Publisher Correction: Regulation of the MLH1–MLH3 endonuclease in meiosis

Cannavó, Elda; Sanchez, Aurore; Anand, Roopesh; Ranjha, Lepakshi; Hugener, Jannik; Adam, Céline; Acharya, Ananya; Weyland, Nicolas; Aran-Guiu, Xavier; Charbonnier, Jean-Baptiste; Hoffmann, Eva R.; Borde, Valérie; Matos, Joao; Ćejka, Petr

doi:10.1038/s41586-020-03111-9

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“…The eukaryotic MMR complex is composed of four heterodimers containing MutSb (MSH2-MSH3), MutSa (MSH2-MSH6), MutLa (MLH1-PMS1/PMS2) and MutLc (MLH1-MLH3). Research in yeast and mammals show that, as an endonuclease, MutLc plays a major role in meiotic recombination by forming a complex with MutSc (Msh4-Msh5) to promote meiotic crossovers (COs) (Cannavo et al, 2020;Flores-Rozas and Kolodner, 1998;Kadyrova et al, 2020;Kolodner, 2016;Lipkin et al, 2000;Nishant et al, 2010;Ranjha et al, 2014;Wang et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Rice MutLγ, the MLH1–MLH3 heterodimer, participates in the formation of type I crossovers and regulation of embryo sac fertility

Mao

Zheng

Huang

et al. 2021

Plant Biotechnology Journal

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The development of embryo sacs is crucial for seed production in plants, but the genetic basis regulating the meiotic crossover formation in the macrospore and microspore mother cells remains largely unclear. Here, we report the characterization of a spontaneous rice female sterile variation 1 mutant (fsv1) that showed severe embryo sacs abortion with low seed-setting rate. Through map-based cloning and functional analyses, we isolated the causal gene of fsv1, OsMLH3 encoding a MutL-homolog 3 protein, an ortholog of HvMLH3 in barley and AtMLH3 in Arabidopsis. OsMLH3 and OsMLH1 (MutL-homolog 1) interact to form a heterodimer (MutLc) to promote crossover formation in the macrospore and microspore mother cells and development of functional megaspore during meiosis, defective OsMLH3 or OsMLH1 in fsv1 and CRISPR/Cas9based knockout lines results in reduced type I crossover and bivalent frequency. The fsv1 and OsMLH3-knockout lines are valuable germplasms for development of female sterile restorer lines for mechanized seed production of hybrid rice.

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