Pubertal consumption of R. badensis subspecies acadiensis modulates LPS-induced immune responses and gut microbiome dysbiosis in a sex-specific manner

Yahfoufi, Nour; Kadamani, Anthony K.; Aly, Sarah; Sharani, Sara Al; Liang, Jacky; Butcher, James; Stintzi, Alain; Matar, Chantal; Ismail, Nafissa

doi:10.1016/j.bbi.2022.09.013

Cited by 11 publications

(16 citation statements)

References 77 publications

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“…According to the results, it appears that the presence of LPS has a discernible effect on the diversity and composition of the microbial population within the intestinal tract. This observation is consistent with a significant body of previous research that has also demonstrated this phenomenon [ 43 , 44 , 45 , 46 , 47 ]. LPS, which are commonly found in the cell wall of Gram-negative bacteria, have been shown to elicit significant alterations in the composition and structure of the gut microbiota.…”

Section: Discussionsupporting

confidence: 93%

The Water Extract of Ampelopsis grossedentata Alleviates Oxidative Stress and Intestinal Inflammation

Wang

Jiang

et al. 2023

Antioxidants

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Oxidative stress is recognized as a significant contributor to the development and progression of inflammation and disruptions in the balance of gut microflora, commonly referred to as intestinal dysbiosis. It is crucial that safe and effective antioxidant and anti-inflammatory agents are identified to address these conditions. Ampelopsis grossedentata, a natural plant abundant in flavonoids and primarily found in southern China, has demonstrated potent antioxidant properties. However, the extent to which flavonoids in A. grossedentata impact intestinal inflammation and alter the composition of the gut microbiome remains to be fully understood. The purpose of this study was to explore the potential benefits of using A. grossedentata as an antioxidant and anti-inflammatory agent in the context of intestinal inflammation, both in vitro and in vivo. We first conducted an initial comparison of the effects of dihydromyricetin (DMY), an alcohol extract of A. grossedentata (AEA, 82% total flavonoids), and a water extract of A. grossedentata (WEA, 57% total flavonoids) on the cell viability and intestinal barrier integrity of porcine epithelial cells IPEC-J2. Although the total flavonoid content is much lower in WEA than in AEA, the results show that they have similar effects. Subsequently, the antioxidant properties of WEA were compared with those of commonly utilized antioxidants in vitro. Lastly, the antioxidant and anti-inflammatory properties of WEA, as well as its impacts on gut microbiota, were evaluated in animal models, including mice and Drosophila. In summary, the results of our study indicate that WEA, due to its antioxidant properties, exhibits a protective effect on the intestinal barrier function in porcine epithelial cell line IPEC-J2. Additionally, WEA demonstrates a positive correlation with DPPH, ABTS radical scavenging rate, FRAP, and reducing power under in vitro settings. Furthermore, WEA was shown to effectively alleviate oxidative stress in animal models by reducing the levels of pro-inflammatory cytokines and increasing the antioxidant enzyme activity in the liver, as well as by activating the Nrf2 signaling pathway in the duodenum. Additionally, WEA was able to regulate gut microbiota, promoting the growth of beneficial bacteria and inhibiting harmful microbes, as well as extending the lifespan of Drosophila. Overall, these findings suggest that WEA may serve as a valuable dietary supplement for addressing oxidative stress and inflammation through its anti-inflammatory and prebiotic effects, which are conferred via the Nrf2/Keap1 pathway.

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Section: Discussionsupporting

confidence: 93%

The Water Extract of Ampelopsis grossedentata Alleviates Oxidative Stress and Intestinal Inflammation

Wang

Jiang

et al. 2023

Antioxidants

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“…Recent studies have reported that LPS induces innate immune activation 29 , 30 . Therefore, we explored the composition of immune cells in different LRHG prognostic indicator groups, and also used Wilcoxon test for correlation analysis.…”

Section: Resultsmentioning

confidence: 99%

Bacterial lipopolysaccharide related genes signature as potential biomarker for prognosis and immune treatment in gastric cancer

Yuan,

Zhang,

et al. 2023

Sci Rep

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The composition of microbial microenvironment is an important factor affecting the development of tumor diseases. However, due to the limitations of current technological levels, we are still unable to fully study and elucidate the depth and breadth of the impact of microorganisms on tumors, especially whether microorganisms have an impact on cancer. Therefore, the purpose of this study is to conduct in-depth research on the role and mechanism of prostate microbiome in gastric cancer (GC) based on the related genes of bacterial lipopolysaccharide (LPS) by using bioinformatics methods. Through comparison in the Toxin Genomics Database (CTD), we can find and screen out the bacterial LPS related genes. In the study, Venn plots and lasso analysis were used to obtain differentially expressed LPS related hub genes (LRHG). Afterwards, in order to establish a prognostic risk score model and column chart in LRHG features, we used univariate and multivariate Cox regression analysis for modeling and composition. In addition, we also conducted in-depth research on the clinical role of immunotherapy with TMB, MSI, KRAS mutants, and TIDE scores. We screened 9 LRHGs in the database. We constructed a prognostic risk score and column chart based on LRHG, indicating that low risk scores have a protective effect on patients. We particularly found that low risk scores are beneficial for immunotherapy through TIDE score evaluation. Based on LPS related hub genes, we established a LRHG signature, which can help predict immunotherapy and prognosis for GC patients. Bacterial lipopolysaccharide related genes can also be biomarkers to predict progression free survival in GC patients.

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“…Rouxiella badensis is a Gram-negative bacterium in the family Yersiniaceae ( 1 ) found associated with soil and plant ecosystems ( 2 ), with emerging applications including post-harvest preservation of berries ( 3 ), a human antidepressant probiotic ( 4 – 6 ), biosynthesis of L-asparaginase ( 7 ), and uncharacterized antibiotics ( 8 ). High-quality genome sequences, such as described here, allow improved identification of metabolic pathways and secondary metabolite prediction ( 9 , 10 ).…”

Section: Announcementmentioning

confidence: 99%

Complete genome sequence of Australian soil bacterium Rouxiella badensis DAR84756 resolved with Oxford Nanopore long-read and Illumina sequences

Paul,

Anderson,

Maynard

et al. 2024

Microbiol Resour Announc

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Pubertal consumption of R. badensis subspecies acadiensis modulates LPS-induced immune responses and gut microbiome dysbiosis in a sex-specific manner

Cited by 11 publications

References 77 publications

The Water Extract of Ampelopsis grossedentata Alleviates Oxidative Stress and Intestinal Inflammation

The Water Extract of Ampelopsis grossedentata Alleviates Oxidative Stress and Intestinal Inflammation

Bacterial lipopolysaccharide related genes signature as potential biomarker for prognosis and immune treatment in gastric cancer

Complete genome sequence of Australian soil bacterium Rouxiella badensis DAR84756 resolved with Oxford Nanopore long-read and Illumina sequences

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