The Water Extract of Ampelopsis grossedentata Alleviates Oxidative Stress and Intestinal Inflammation

Abstract

Oxidative stress is recognized as a significant contributor to the development and progression of inflammation and disruptions in the balance of gut microflora, commonly referred to as intestinal dysbiosis. It is crucial that safe and effective antioxidant and anti-inflammatory agents are identified to address these conditions. Ampelopsis grossedentata, a natural plant abundant in flavonoids and primarily found in southern China, has demonstrated potent antioxidant properties. However, the extent to which flavonoids in A. grossedentata impact intestinal inflammation and alter the composition of the gut microbiome remains to be fully understood. The purpose of this study was to explore the potential benefits of using A. grossedentata as an antioxidant and anti-inflammatory agent in the context of intestinal inflammation, both in vitro and in vivo. We first conducted an initial comparison of the effects of dihydromyricetin (DMY), an alcohol extract of A. grossedentata (AEA, 82% total flavonoids), and a water extract of A. grossedentata (WEA, 57% total flavonoids) on the cell viability and intestinal barrier integrity of porcine epithelial cells IPEC-J2. Although the total flavonoid content is much lower in WEA than in AEA, the results show that they have similar effects. Subsequently, the antioxidant properties of WEA were compared with those of commonly utilized antioxidants in vitro. Lastly, the antioxidant and anti-inflammatory properties of WEA, as well as its impacts on gut microbiota, were evaluated in animal models, including mice and Drosophila. In summary, the results of our study indicate that WEA, due to its antioxidant properties, exhibits a protective effect on the intestinal barrier function in porcine epithelial cell line IPEC-J2. Additionally, WEA demonstrates a positive correlation with DPPH, ABTS radical scavenging rate, FRAP, and reducing power under in vitro settings. Furthermore, WEA was shown to effectively alleviate oxidative stress in animal models by reducing the levels of pro-inflammatory cytokines and increasing the antioxidant enzyme activity in the liver, as well as by activating the Nrf2 signaling pathway in the duodenum. Additionally, WEA was able to regulate gut microbiota, promoting the growth of beneficial bacteria and inhibiting harmful microbes, as well as extending the lifespan of Drosophila. Overall, these findings suggest that WEA may serve as a valuable dietary supplement for addressing oxidative stress and inflammation through its anti-inflammatory and prebiotic effects, which are conferred via the Nrf2/Keap1 pathway.

Polystyrene Microplastics Causes Diarrhea and Impairs Intestinal Angiogenesis through the ROS/METTL3 Pathway

“…58 The Nrf2/Keap1 signaling pathway is vital for defense against oxidative stress. 59 In this study, polystyrene MPs exposure upregulated NRF2 protein level and downregulated KEAP1, suggesting impairment of the Nrf2/Keap1 pathway and consequent intestinal oxidative damage. 60 High levels of oxidative stress can trigger inflammation and endoplasmic reticulum stress.…”

“…Nrf2, a basic leucine zipper transcription factor, is crucial in regulating cellular redox homeostasis by inducing detoxifying and antioxidant enzymes . The Nrf2/Keap1 signaling pathway is vital for defense against oxidative stress . In this study, polystyrene MPs exposure upregulated NRF2 protein level and downregulated KEAP1, suggesting impairment of the Nrf2/Keap1 pathway and consequent intestinal oxidative damage .…”

Effects of Bacillus coagulans TBC169 on gut microbiota and metabolites in gynecological laparoscopy patients

“…It responds to oxidative stress by promoting cell growth and preserving membrane integrity ( Jiao et al, 2015 ). The occurrence and development of inflammation caused by oxidative stress are important factors in disrupting the balance of gut microbiota ( Wang et al, 2023 ). In this study, we speculated that the down-regulation of Glu in patients taking BCTL may be attributed to increased absorption by intestinal epithelial cells.…”

Therapeutic potential of natural products in schistosomiasis-associated liver fibrosis

“…Wang , also known as vine tea, is a plant of the genus Ampelopsis in the family Vitaceae, mainly distributed in southern China. The pharmacological effects of vine tea are mainly summarized as anti-inflammatory and analgesic, hepatoprotective, hypotensive, hypolipidemic, antitumor, and anti-aging ( Xie et al, 2019 ; Tong et al, 2020 ; Wang et al, 2023 ). Flavonoids are the main efficacy components of vine tea, which includes dihydromyricetin, myricitrin, myricetin, quercetin, rutin, and kaempferol ( Xu et al, 2012 ).…”