PTGS2 down-regulation in cumulus cells of infertile women with endometriosis

Luz, Caroline Mantovani da; Broi, Michele Gomes Da; Donabela, Flávia Cappello; Paz, Cláudia Cristina Paro de; Meola, Juliana; Navarro, Paula Andrea

doi:10.1016/j.rbmo.2017.06.021

Cited by 29 publications

(18 citation statements)

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“…PTGS2, involved in muscle contraction, was observed to be highly downregulated as well. Its low levels of expression may be involved in infertility [ 61 ].…”

Section: Discussionmentioning

confidence: 99%

Muscle Cell Morphogenesis, Structure, Development and Differentiation Processes Are Significantly Regulated during Human Ovarian Granulosa Cells In Vitro Cultivation

Dompe

Kranc

Jopek

et al. 2020

JCM

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Granulosa cells (GCs) have many functions and are fundamental for both folliculogenesis and oogenesis, releasing hormones and communicating directly with the oocyte. Long-term in vitro cultures of GCs show significant stem-like characteristics. In the current study, RNA of human ovarian granulosa cells was collected at 1, 7, 15 and 30 days of long-term in vitro culture. Understanding the process of differentiation of GCs towards different cell lineages, as well as the molecular pathways underlying these mechanisms, is fundamental to revealing other possible stemness markers of this type of cell. Identifying new markers of GC plasticity may help to understand the aetiology and recurrence of a wide variety of diseases and health conditions and reveal possible clinical applications of the ovarian tissue cells, affecting not only the reproductive ability but also sex hormone production. Granulosa cells were the subject of this study, as they are readily available as remnant material leftover after in vitro fertilisation procedures and exhibit significant stem-like characteristics in culture. The change in gene expression was investigated through a range of molecular and bioinformatic analyses. Expression microarrays were used, allowing the identification of groups of genes typical of specific cellular pathways. This candidate gene study focused on ontological groups associated with muscle cell morphogenesis, structure, development and differentiation, namely, “muscle cell development”, “muscle cell differentiation”, “muscle contraction”, “muscle organ development”, “muscle organ morphogenesis”, “muscle structure development”, “muscle system process” and “muscle tissue development”. The results showed that the 10 most upregulated genes were keratin 19, oxytocin receptor, connective tissue growth factor, nexilin, myosin light chain kinase, cysteine and glycine-rich protein 3, caveolin 1, actin, activating transcription factor 3 and tropomyosin, while the 10 most downregulated consisted of epiregulin, prostaglandin-endoperoxide synthase 2, transforming growth factor, interleukin, collagen, 5-hydroxytryptmine, interleukin 4, phosphodiesterase, wingless-type MMTV integration site family and SRY-box 9. Moreover, ultrastructural observations showing heterogeneity of granulosa cell population are presented in the study. At least two morphologically different subpopulations were identified: large, light coloured and small, darker cells. The expression of genes belonging to the mentioned ontological groups suggest the potential ability of GCs to differentiate and proliferate toward muscle lineage, showing possible application in muscle regeneration and the treatment of different diseases.

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“…PTGS2, involved in muscle contraction, was observed to be highly downregulated as well. Its low levels of expression may be involved in infertility [ 61 ].…”

Section: Discussionmentioning

confidence: 99%

Muscle Cell Morphogenesis, Structure, Development and Differentiation Processes Are Significantly Regulated during Human Ovarian Granulosa Cells In Vitro Cultivation

Dompe

Kranc

Jopek

et al. 2020

JCM

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“…In a randomized study, Cruz and colleagues (2017) showed that the type of gonadotropin used during controlled ovarian stimulation (follicle-stimulating hormone (FSH), urinary FSH, or human menopausal gonadotropin (hMG)) induce differential gene expression in human CCs [ 50 ]. Moreover, PTGS2 was shown to be down-regulated in CCs of infertile women with endometriosis [ 51 ]. Patient age affects differential mRNA gene expression in CCs [ 52 – 54 ].…”

Section: Discussionmentioning

confidence: 99%

Is there a correlation between follicle size and gene expression in cumulus cells and is gene expression an indicator of embryo development?

Kahraman

Çetinkaya

et al. 2018

Reprod Biol Endocrinol

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BackgroundIn an article published in 2017, we discussed the results of the first part of our study into the morphokinetic development of embryos in relation to follicle diameter and homogeneity of follicular development. Our findings showed that embryos coming from small follicles in heterogeneous cycles had significantly higher rates of arrest or failure to reach blastocyst than embryos coming from large follicles in homogenous cycles. The aim of this further study was to investigate the relationship between follicular size and gene expression of cumulus cells (CCs) and evaluate whether gene expression could be an indicator of embryo development.MethodsThis study was based on 2495 COCs from 184 patients. CC expressions of five genes (TNFAIP6, PTGS2, HAS2, PTX3 and GDF9) were studied by generalized linear mixed models (GLMMs) regarding follicular size. CC expressions were then separately analysed regarding patient-specific variables (age, BMI, AMH and follicular size) in relation to embryos reaching blastocyst (eRB) or top or good quality blastocysts (TQ + GQ) using GLMMs with logit link.ResultsFollicular size significantly correlated with the potential of an oocyte to develop into a blastocyst: oocytes developing from large follicles were more than twice as likely to develop into an eRB than oocytes from small follicles (p < 0.001). Gene expression of HAS2 and GDF9 correlated with blastocyst quality when separately evaluated with follicular size and the patient specific variables of age, BMI and AMH. However, no such correlation was found in other gene expressions studied.ConclusionsOur findings suggest that differences in the expression of genes studied could be related to follicular size rather than to embryo quality. Although gene expression of HAS2 and GDF9 correlated with blastocyst quality, the only variable correlating with eRB and TQ and GQ blastocysts for each of these five models was follicular size.Trial registrationThis prospective cohort study was registered at clinicaltrials.gov (NCT02230449).Electronic supplementary materialThe online version of this article (10.1186/s12958-018-0388-0) contains supplementary material, which is available to authorized users.

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“…JOSD1 depletion can lead to severe apoptosis in gynaecological cancer cells both in vivo and in vitro (29). The PTGS2 gene, which encodes cyclooxygenase 2 (COX-2), is deregulated in endometriotic lesions and plays an important role in the acquisition of oocyte competence (30). IRAK is responsible for the regulation of microbial colonization of tumors and STAT3 protein stability in tumor cells, leading to tumor cell proliferation (31).…”

Section: Cre Induces Up-and Down-regulation Of Gene Expression Inmentioning

confidence: 99%

The Ethanol Crude Extraction of Cyperus Rotundus Regulates Apoptosis-associated Gene Expression in HeLa Human Cervical Carcinoma Cells In Vitro

et al. 2019

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Background/Aim: Cervical cancer is considered poorly chemo-sensitive in women and its treatment remains unsatisfactory. Cyperus rotundus is used in Chinese medicine as a therapeutic agent for women's disease. The effects and molecular mechanisms of the ethanol extraction of C. rotundus (CRE) on cervical cancer remain unclear. We aimed to explore the mechanisms and genetic influence of CRE on cervical cancer. Materials and Methods: HeLa, human cervical cancer cells were treated with various doses of CRE and changes in cell morphology and cell viability were assessed using microscopy and flow cytometry. Finally, we performed a microarray analysis to scan related genes. Results: The treatment of CRE on HeLa cells caused morphological changes and induced chromatin condensation. DNA microarray analysis showed that CRE led to up-regulation of 449 genes and downregulation of 484 genes, which were classified in several interaction pathways. Conclusion: CRE changed HeLa cell morphology and induced gene expression which associated with apoptosis and cell-cycle arrest. These results provide important information at the transcription level for targeting treatments of human cervical cancer.Cyperus rotundus L. (Cyperaceae) has been extensively used in traditional medicine in Asia, Africa, and Europe. It is also known as nutgrass and is cultivated widely in tropical, subtropical and temperate regions. It is prescribed for gynecological disorders, including dysmenorrhea and irregular menstruation, while its rhizomes (rootstalks) have been used as sedatives and analgesics (1, 2). Recently, many studies have demonstrated that its rhizomes have a vast range of biological and pharmacological functions, including anti-oxidant, antiinflammatory, anti-diabetic, anti-allergic, anti-nociceptive and anti-cancer effects (2-4). The active chemical constituents of C.

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PTGS2 down-regulation in cumulus cells of infertile women with endometriosis

Cited by 29 publications

References 60 publications

Muscle Cell Morphogenesis, Structure, Development and Differentiation Processes Are Significantly Regulated during Human Ovarian Granulosa Cells In Vitro Cultivation

Muscle Cell Morphogenesis, Structure, Development and Differentiation Processes Are Significantly Regulated during Human Ovarian Granulosa Cells In Vitro Cultivation

Is there a correlation between follicle size and gene expression in cumulus cells and is gene expression an indicator of embryo development?

The Ethanol Crude Extraction of Cyperus Rotundus Regulates Apoptosis-associated Gene Expression in HeLa Human Cervical Carcinoma Cells In Vitro

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