The Ethanol Crude Extraction of Cyperus Rotundus Regulates Apoptosis-associated Gene Expression in HeLa Human Cervical Carcinoma Cells In Vitro

Abstract

Background/Aim: Cervical cancer is considered poorly chemo-sensitive in women and its treatment remains unsatisfactory. Cyperus rotundus is used in Chinese medicine as a therapeutic agent for women's disease. The effects and molecular mechanisms of the ethanol extraction of C. rotundus (CRE) on cervical cancer remain unclear. We aimed to explore the mechanisms and genetic influence of CRE on cervical cancer. Materials and Methods: HeLa, human cervical cancer cells were treated with various doses of CRE and changes in cell morphology and cell viability were assessed using microscopy and flow cytometry. Finally, we performed a microarray analysis to scan related genes. Results: The treatment of CRE on HeLa cells caused morphological changes and induced chromatin condensation. DNA microarray analysis showed that CRE led to up-regulation of 449 genes and downregulation of 484 genes, which were classified in several interaction pathways. Conclusion: CRE changed HeLa cell morphology and induced gene expression which associated with apoptosis and cell-cycle arrest. These results provide important information at the transcription level for targeting treatments of human cervical cancer.Cyperus rotundus L. (Cyperaceae) has been extensively used in traditional medicine in Asia, Africa, and Europe. It is also known as nutgrass and is cultivated widely in tropical, subtropical and temperate regions. It is prescribed for gynecological disorders, including dysmenorrhea and irregular menstruation, while its rhizomes (rootstalks) have been used as sedatives and analgesics (1, 2). Recently, many studies have demonstrated that its rhizomes have a vast range of biological and pharmacological functions, including anti-oxidant, antiinflammatory, anti-diabetic, anti-allergic, anti-nociceptive and anti-cancer effects (2-4). The active chemical constituents of C.

In vitro and in vivo anthelmintic and chemical studies of Cyperus rotundus L. extracts

“…Furthermore, it has been used in folk medicine as a diuretic, aphrodisiac, sedative, carminative, and a remedy for renal colic and dysentery [ 11 , 12 ]. Also, C. rotundus has many biological and pharmacological activities (in vitro and in vivo) such as cytotoxic [ 13 ], antimicrobial [ 14 ], anti-inflammatory [ 15 ], anti-allergic [ 16 ],anti-diarrheal [ 17 ], and hepatoprotective [ 18 ]. The previous phytochemical studies of C. rotundus have revealed the presence of several types of secondary metabolites, such as sesquiterpenes, flavonoids, iridoids, phenylpropanoids, furochromones, phenolic acids, alkaloids, steroids, and saponins [ 8 , 19 – 21 ].…”

Picrasma quassioides Extract Elevates the Cervical Cancer Cell Apoptosis Through ROS-Mitochondrial Axis Activated p38 MAPK Signaling Pathway

“…Cancer is a complex process involving a series of proliferative signals, including escape from growth inhibition, resistance to cell death, and uncontrolled proliferation as well as increased invasion and metastasis of cancer cells. A great focus has been on the development of new anti-tumor drugs, including natural plant drugs and their derivatives (6,31). In fact, herbal medicinal have been tested as potential treatment modalities for a variety of cancers, including leukemia, ovarian, testicular, lung, liver, esophageal, stomach, colon, and rectum cancer (32)(33)(34).…”

Demethoxycurcumin Inhibits In Vivo Growth of Xenograft Tumors of Human Cervical Cancer Cells

“…Cells were cultured in DMEM containing 10% FBS, 2 mM Lglutamine, 100 Units/ml penicillin, and 100 μg/ml streptomycin. Cells were grown in a 75 cm 2 culture flasks under a humidified 5% CO 2 and 95% air at 37˚C at 1 atmosphere as described previously (22). All animals were randomly divided into three different treatment groups (control, 30 mg/kg DMC, and 50 mg/kg DMC; n=8 for each group) when the tumor volume reached 100-120 mm 3 in each mouse.…”