Pterostilbene prevents methylglyoxal-induced cytotoxicity in endothelial cells by regulating glyoxalase, oxidative stress and apoptosis

Tang, Dan; Xiao, Wei; Gu, Wen-Ting; Zhang, Zhi-Tong; Xu, Shuhong; Chen, Zhiquan; Xu, Youhua; Zhang, Luyong; Wang, Shumei; Nie, Hong

doi:10.1016/j.fct.2021.112244

Cited by 18 publications

(16 citation statements)

References 31 publications

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“…MGO is a reactive cytotoxic dicarbonyl compound formed during the metabolism of glucose and fructose, and its reactive activity is 20,000 times higher than that of glucose [8–10] . Lipid peroxidation, DNA degradation, and the absorption from food are the other sources of MGO [11] .…”

Section: Introductionmentioning

confidence: 99%

Potential Mechanisms of Methylglyoxal‐Induced Human Embryonic Kidney Cells Damage: Regulation of Oxidative Stress, DNA Damage, and Apoptosis

Liú

Cheng

Tang

et al. 2022

Chemistry & Biodiversity

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Methylglyoxal (MGO) is a reactive carbonyl species that can cause cellular damage and is closely related to kidney disease, especially diabetic nephropathy. The toxic effect of MGO (0.5, 1, and 2 mM) on human embryonic kidney (HEK293) cells and its underlying mechanisms were explored in this study. Cell viability, apoptosis and the signaling pathways were measured with MTT, fluorescent staining and western blot experiments, the results showed that MGO could induce oxidative stress and cell inflammation, the level of reactive oxygen species (ROS) increased, and p38MAPK, JNK and NF‐κB signaling pathways were activated. Meanwhile, MGO also induced DNA damage. The expression of DNA oxidative damage marker 8‐hydroxy‐2’‐deoxyguanosine (8‐OHdG) increased, the expression of double‐strand break marker γH2AX increased significantly, and ATM/Chk2/p53 DNA damage response signaling pathway was activated. Furthermore, the expression of the receptor for advanced glycation end products (RAGE) also increased. Finally, mitochondrial membrane potential (MMP) decreased, fluorescence intensity of Hoechst33258 increased, and the protein expression ratio of Bax/Bcl‐2 increased significantly after the treatment of MGO. These results demonstrated that MGO might induce HEK293 cells damage by regulating oxidative stress, inflammation, DNA damage, and cell apoptosis, which revealed the specific mechanisms of MGO‐induced damage to HEK293 cells.

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Section: Introductionmentioning

confidence: 99%

Potential Mechanisms of Methylglyoxal‐Induced Human Embryonic Kidney Cells Damage: Regulation of Oxidative Stress, DNA Damage, and Apoptosis

Liú

Cheng

Tang

et al. 2022

Chemistry & Biodiversity

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“…In 2021, pterostilbene was reported to prevent oxidative stress and apoptosis in endothelial cells treated with methylglyoxal (MGO). 104 In this study, MGO-elevated ROS was suppressed by HO-1, CAT, and SOD, which were promoted by Nrf2 translocation, subsequently preventing the loss of mitochondrial membrane potential and activation of the caspase-9 cascade. Rescuing mitochondrial damage is a major strategy for preventing some diseases, such as neurodegenerative diseases, mainly through Nrf2 activation.…”

Section: Pterostilbenementioning

confidence: 56%

“…Furthermore, knockdown of Nrf2 abolished the antioxidative effect of pterostilbene. In 2021, pterostilbene was reported to prevent oxidative stress and apoptosis in endothelial cells treated with methylglyoxal (MGO) . In this study, MGO-elevated ROS was suppressed by HO-1, CAT, and SOD, which were promoted by Nrf2 translocation, subsequently preventing the loss of mitochondrial membrane potential and activation of the caspase-9 cascade.…”

Section: Pterostilbenementioning

confidence: 75%

Recent Advances in Health Benefits of Stilbenoids

Koh

Pan

2021

J. Agric. Food Chem.

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Biological targeting or molecular targeting is the main strategy in drug development and disease prevention. However, the problem of “off-targets” cannot be neglected. Naturally derived drugs are preferred over synthetic compounds in pharmaceutical markets, and the main goals are high effectiveness, lower cost, and fewer side effects. Single-target drug binding may be the major cause of failure, as the pathogenesis of diseases is predominantly multifactorial. Naturally derived drugs are advantageous because they are expected to have multitarget effects, but not off-targets, in disease prevention or therapeutic actions. The capability of phytochemicals to modulate molecular signals in numerous diseases has been widely discussed. Among them, stilbenoids, especially resveratrol, have been well-studied, along with their potential molecular targets, including AMPK, Sirt1, NF-κB, PKC, Nrf2, and PPARs. The analogues of resveratrol, pterostilbene, and hydroxylated-pterostilbene may have similar, if not more, potential biological targeting effects compared with their original counterpart. Furthermore, new targets that have been discussed in recent studies are reviewed in this paper.

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“…It is a powerful defence system against glycation reactions in the human body ( 63 , 64 ). Studies have shown that Pterostylus membranaceus (PTS) ( 121 ) and Lithospermum erythrorhizon root ( 122 ) activate the glyoxalase defence system by upregulating the glyoxalase I (GLO-1) expression level and increasing the glutathione (GSH) content, thus reducing the production of AGEs and improving the skin condition.…”

Section: Pathways Inhibiting Advanced Glycation End Productsmentioning

confidence: 99%

Advanced Glycation End Products in the Skin: Molecular Mechanisms, Methods of Measurement, and Inhibitory Pathways

Chen

Zhang

et al. 2022

Front. Med.

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Advanced glycation end products (AGEs) are a series of stable compounds produced under non-enzymatic conditions by the amino groups of biomacromolecules and the free carbonyl groups of glucose or other reducing sugars commonly produced by thermally processed foods. AGEs can cause various diseases, such as diabetes, atherosclerosis, neurodegeneration, and chronic kidney disease, by triggering the receptors of AGE (RAGEs) in the human body. There is evidence that AGEs can also affect the different structures and physiological functions of the skin. However, the mechanism is complicated and cumbersome and causes various harms to the skin. This article aims to identify and summarise the formation and characteristics of AGEs, focussing on the molecular mechanisms by which AGEs affect the composition and structure of normal skin substances at different skin layers and induce skin issues. We also discuss prevention and inhibition pathways, provide a systematic and comprehensive method for measuring the content of AGEs in human skin, and summarise and analyse their advantages and disadvantages. This work can help researchers acquire a deeper understanding of the relationship between AGEs and the skin and provides a basis for the development of effective ingredients that inhibit glycation.

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Pterostilbene prevents methylglyoxal-induced cytotoxicity in endothelial cells by regulating glyoxalase, oxidative stress and apoptosis

Cited by 18 publications

References 31 publications

Potential Mechanisms of Methylglyoxal‐Induced Human Embryonic Kidney Cells Damage: Regulation of Oxidative Stress, DNA Damage, and Apoptosis

Potential Mechanisms of Methylglyoxal‐Induced Human Embryonic Kidney Cells Damage: Regulation of Oxidative Stress, DNA Damage, and Apoptosis

Recent Advances in Health Benefits of Stilbenoids

Advanced Glycation End Products in the Skin: Molecular Mechanisms, Methods of Measurement, and Inhibitory Pathways

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