Pterostilbene prevents intestinal ischemia reperfusion injury in wistar rats via modulation of antioxidant defense and inflammation

Sun, Yang; Yang, Xu; Wang, Guonian

doi:10.4314/tjpr.v14i8.9

Cited by 6 publications

(12 citation statements)

References 22 publications

(18 reference statements)

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“…In this model of intestinal I/R injury, cashew nuts administration was able to decrease lipid peroxidation, ROS generation, and protein carbonyl content. Several works showed the decrease in CAT and SOD levels and GST, GPx, and GSH activities in both patients and experimental models, confirming the role of oxidative stress in intestinal ischemia pathogenesis [ 34 ]. These enzymes help cells to repair the cellular membranes damaged by ROS.…”

Section: Discussionmentioning

confidence: 78%

“…Cashew nuts (100 mg/kg body) were administered for 3 consecutive days through gavage. After the treatment schedule, the rats were fasted for 12 h. The induction of intestinal ischemia and reperfusion injury was performed as already described [ 34 ]. After anesthesia induction and midline laparotomy, ischemia and reperfusion injury were induced by clamping the superior mesenteric artery.…”

Section: Methodsmentioning

confidence: 99%

“…Ileum tissues were homogenized, and the supernatants were collected for determining total glutathione (Glutathione Assay Kit; Trevigen Inc., Gaithersburg, MD, USA). The results of GSH levels were expressed as nanomole per milligram of protein [ 34 ]. SOD activity was determined on ileum tissue homogenate according to the principle of nitro blue tetrazolium reduction assay [ 58 ].…”

Section: Methodsmentioning

confidence: 99%

“…The catalase (CAT) activity was estimated by decreased in absorbance of H 2 O 2 at 240 nm [ 59 ]. The GPx was performed as described [ 34 ]. Oxidized glutathione (GSSG) is reduced by glutathione reductase and NADPH.…”

Section: Methodsmentioning

confidence: 99%

“…GPx activity is expressed as U/mg of protein. The glutathione-S-transferase (GST) activity was determined spectrophotometrically at 340 nm [ 34 ]. 1 U = amount of enzyme producing 1 mmol of CDNB-GSH conjugate/min.…”

Section: Methodsmentioning

confidence: 99%

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Consumption of Anacardium occidentale L. (Cashew Nuts) Inhibits Oxidative Stress through Modulation of the Nrf2/HO−1 and NF-kB Pathways

et al. 2020

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Ischemia/reperfusion injury is a severe disorder associated with a high mortality. Several antioxidant and pharmacological properties of cashew nuts (Anacardium occidentale L.) and its metabolites from different countries have recently been described. It is a medicinal plant with important therapeutic effects. This study aimed to verify the effect of an oral administration of cashew nuts in a rat model of ischemia/reperfusion (I/R). Adult male rats were subjected to intestinal I/R injury by clamping the superior mesenteric artery for 30 min and then allowing animals to 1 h of reperfusion. Rats subjected to I/R of the gut showed a significant increase in different biochemical markers. In particular, we evaluated lipid peroxidation, tissue myeloperoxidase activity, protein carbonyl content, reactive oxygen species generation and decreased antioxidant enzyme activities. Western blot analysis showed the activation of the NRF2 and NF-kB pathways. Increased immunoreactivity to nitrotyrosine, PARP, P-selectin, and ICAM-1 was observed in the ileum of rats subjected to I/R. Administration of cashew nuts (100 mg/kg) significantly reduced the mortality rate, the fall in arterial blood pressure, and oxidative stress and restored the antioxidant enzyme activities by a mechanism involving both NRF2 and NF-kB pathways. Cashew nuts treatments reduced cytokines plasma levels, nitrotyrosine, and PARP expression as well as adhesion molecules expressions. Additionally, cashew nuts decreased the intestinal barrier dysfunction and mucosal damage, the translocation of toxins and bacteria, which leads to systemic inflammation and associated organs injuries in particular of liver and kidney. Our study demonstrates that cashew nuts administration exerts antioxidant and pharmacological protective effects in superior mesenteric artery occlusion–reperfusion shock.

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Section: Discussionmentioning

confidence: 78%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Consumption of Anacardium occidentale L. (Cashew Nuts) Inhibits Oxidative Stress through Modulation of the Nrf2/HO−1 and NF-kB Pathways

et al. 2020

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Resveratrol and curcumin as protective agents in an experimental rat model of intestinal ischemia and reperfusion

Cucolas

Daneasa

Olteanu

et al. 2016

Can. J. Physiol. Pharmacol.

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The aim of this study was to evaluate the protective effects of resveratrol and curcumin in an experimental rat model of intestinal ischemia-reperfusion (I/R). 48 adult Wistar rats were used: 12 animals undergoing the sham surgery and 36 animals undergoing laparotomy, with 15 minutes of mesentric artery clamping. The animals from the latter groups (n=12) were pre-treated, for a week, with vehicle (CTR), resveratrol (RES) and curcumin (CUR). After one and 6 hours of reperfusion respectively, cyclooxigenase (COX)-2, mucin-1, E-cadherin, nuclear factor (NK)-kB expressions and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) were assessed in the small intestine. Oxidative stress markers were determined in tissue homogenate and serum and histopathological analysis was performed.Pretreatment with RES decreased the expression of COX-2 and NF-kB at both intervals and increased E-cadherine (p<0.05) and mucin-1 production, after one hour. CUR had a beneficial effect on COX-2, NF-kB and E-cadherine expressions, both after one and 6 hours (p<0.0001). The two compounds increased TRAIL levels and had a protective effect on oxidative stress and histopatological lesions, both after one and 6 hours. Our results suggested that RES and CUR had beneficial effects in intestinal ischemia and may represent a promising option for complementary treatment of this pathological condition.

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Pterostilbene Confers Protection against Diquat-Induced Intestinal Damage with Potential Regulation of Redox Status and Ferroptosis in Broiler Chickens

Chen

Wang

et al. 2023

Oxidative Medicine and Cellular Longevity

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Oxidative stress causes damage to macromolecules, including proteins, DNA, and lipid, and has been recognized as a crucial driver of the onset and progression of several intestinal disorders. Pterostilbene, one of the natural antioxidants, has attracted considerable attention owing to its multiple biological activities. In the present study, we established an oxidative stress model in broiler chickens via injection with diquat to investigate whether pterostilbene could attenuate diquat-induced intestinal damage and reveal the underlying mechanisms. We found that diquat-induced decreases in the activities of superoxide dismutase and glutathione peroxidase and the level of reduced glutathione and the increase in hydrogen peroxide content in plasma and jejunum were significantly alleviated by pterostilbene ( P < 0.05 ). Pterostilbene supplementation also decreased intestinal permeability and jejunal apoptosis rate, improved jejunal villus height and the ratio of villus height to crypt depth, and promoted the transcription and translation of jejunal tight junction proteins occludin and zona occludens 1 in diquat-challenged broilers ( P < 0.05 ). Furthermore, pterostilbene reversed diquat-induced mitochondrial injury in the jejunum, as indicated by the decreased reactive oxygen species level and elevated activities of superoxide dismutase 2 and mitochondrial respiratory complexes ( P < 0.05 ). Importantly, administering pterostilbene maintained iron homeostasis, inhibited lipid peroxidation, and regulated the expression of the markers of ferroptosis in the jejunum of diquat-exposed broilers ( P < 0.05 ). The nuclear factor erythroid 2-related factor 2 signaling pathway in the jejunum of diquat-exposed broilers was also activated by pterostilbene ( P < 0.05 ). In conclusion, our study provides evidence that pterostilbene alleviates diquat-induced intestinal mucosa injury and barrier dysfunction by strengthening antioxidant capacity and regulating ferroptosis of broiler chickens.

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Pterostilbene prevents intestinal ischemia reperfusion injury in wistar rats via modulation of antioxidant defense and inflammation

Cited by 6 publications

References 22 publications

Consumption of Anacardium occidentale L. (Cashew Nuts) Inhibits Oxidative Stress through Modulation of the Nrf2/HO−1 and NF-kB Pathways

Consumption of Anacardium occidentale L. (Cashew Nuts) Inhibits Oxidative Stress through Modulation of the Nrf2/HO−1 and NF-kB Pathways

Resveratrol and curcumin as protective agents in an experimental rat model of intestinal ischemia and reperfusion

Pterostilbene Confers Protection against Diquat-Induced Intestinal Damage with Potential Regulation of Redox Status and Ferroptosis in Broiler Chickens

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