Pterostilbene Inhibits Breast Cancer In Vitro Through Mitochondrial Depolarization and Induction of Caspase-Dependent Apoptosis

Alosi, Julie A.; McDonald, Debbie; Schneider, John S.; Privette, Alicia; McFadden, David W.

doi:10.1016/j.jss.2009.07.027

Cited by 75 publications

(65 citation statements)

References 23 publications

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“…Pterostilbene-induced G 0 /G 1 cell cycle arrest has also been observed in AGS human gastric cancer cells [8] as well as in LNCaP human androgen-responsive prostate cancer cells [9]. Pterostilbene has been found to cause S-phase cell cycle arrest in MCF-7 human breast cancer cells [20] and in HL60 human leukemia cells [12].…”

Section: Discussionmentioning

confidence: 89%

“…These findings were supported by the results of the cell cycle analysis, since the proportion of cells in the sub-G1 fraction, indicative of apoptotic DNA cleavage, increased significantly after 48 h of pterostilbene treatment. Pterostilbene-induced apoptotic DNA fragmentation has also been detected in MCF-7 and MDA-MB-231 human breast cancer cells [20], NCI-H460 and SK-MES-1 human lung cancer cells [7] as well as in AGS human gastric cancer cells [8]. …”

Section: Discussionmentioning

confidence: 90%

“…The finding in the present study of the disruption of mitochondrial membrane potential by pterostilbene at the IC 90 concentration was indicative of the intrinsic apoptotic pathway activation in MOLT4 cells. Mitochondrial depolarisation was also detected in pterostilbene-treated MCF-7 and MDA-MB-231 human breast cancer cells [20].…”

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confidence: 87%

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Pterostilbene induces cell cycle arrest and apoptosis in MOLT4 human leukemia cells

Siedlecka-Kroplewska

Jóźwik

Kaszubowska

et al. 2012

Folia Histochem Cytobiol.

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Pterostilbene, a polyphenolic compound present in grapes and other fruits, has been demonstrated to inhibit growth and induce apoptosis and autophagy in some cancer cell types. We found that pterostilbene at the IC 90 concentration of 44 µM inhibited proliferation and induced apoptosis in MOLT4 human leukemia cells. Treatment with pterostilbene resulted in a transient accumulation of cells in the G 0 /G 1 -cell cycle phase followed by the S-phase arrest. Pterostilbene-induced apoptotic death of MOLT4 cells was mediated by caspase-3 activation and was accompanied by the disruption of mitochondrial membrane potential, phosphatidylserine externalisation and internucleosomal DNA fragmentation. Our results suggest that pterostilbene could serve as a potential additional chemotherapeutic agent for the treatment of leukemia.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 90%

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Pterostilbene induces cell cycle arrest and apoptosis in MOLT4 human leukemia cells

Siedlecka-Kroplewska

Jóźwik

Kaszubowska

et al. 2012

Folia Histochem Cytobiol.

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“…Alosi et al (2010) showed that pterostilbene induced a significant concentration-and time-dependent decrease in the breast cancer cell viability. Other studies showed that pterostilbene suppressed multiple signals associated with TPA-induced metastasis (Pan et al 2009) and induced apoptosis (Hasiah et al 2011) on hepatocellular carcinoma cells.…”

Section: Introductionmentioning

confidence: 99%

“…Pterostilbene treatment inhibits the growth of breast cancer MCF-7 and MDA-MB-231 cells in vitro through caspase-dependent apoptosis and alteration of cell cycle. Mitochondrial membrane depolarization and increased superoxide anion may contribute to the activation of downstream effector caspases (Alosi et al 2010). However, little is known about the effects of pterostilbene on the proliferation of hepatoma cells and its mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Anti-proliferative effect of pterostilbene on rat hepatoma cells in culture

2014

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Pterostilbene, a methoxylated analogue of resveratrol, is a natural compound primarily found in blueberries and several types of grapes. However, little is known about the effect of pterostilbene on the proliferation of hepatoma cells and its modes of actions. This study was undertaken to characterize its ability to suppress the proliferation of hepatoma AH109A cells and the possible mechanism(s) involved. Pterostilbene showed a significant and dose-dependent effect on the anti-proliferative activity against AH109A cells. Pterostilbene exerted little or no effect on the proliferation of rat L6 myoblasts and rat skin fibroblasts. Pterostilbeneloaded rat sera could significantly inhibit the proliferation of AH109A cells, which suggests that pterostilbene could be absorbed through gastrointestinal tract and retain its anti-proliferative activity. Pterostilbene arrested the cell cycle of AH109A cells at G0/ G1 phase and reduced the protein expression of cyclindependent kinase 4 and cyclin-dependent kinase 6 dose-dependently. We also found that pterostilbene could significantly increase the intracellular peroxide level of AH109A cells, which may be involved in its anti-proliferative activity.

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Pterostilbene down‐regulates hTERT at physiological concentrations in breast cancer cells: Potentially through the inhibition of cMyc

Daniel

Tollefsbol

2017

J of Cellular Biochemistry

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Human telomerase reverse transcriptase (hTERT) encodes the catalytic subunit of telomerase, which has been shown to be upregulated in many cancers. Pterostilbene is a naturally occurring stilbenoid and phytoalexin found primarily in blueberries that exhibits antioxidant activity and inhibits the growth of various cancer cell types. Therefore, the aim of this study was to determine whether treatment with pterostilbene, at physiologically achievable concentrations, can inhibit the proliferation of breast cancer cells and down-regulate the expression of hTERT. We found that pterostilbene inhibits the cellular proliferation of MCF-7 and MDA-MB-231 breast cancer cells in both a time- and dose-dependent manner, without significant toxicity to the MCF10A control cells. Pterostilbene was also shown to increase apoptosis in both breast cancer cell lines. Dose-dependent cell cycle arrest in G1 and G2/M phase was observed after treatment with pterostilbene in MCF-7 and MDA-231 cells, respectively. hTERT expression was down-regulated after treatment in both a time- and dose-dependent manner. Pterostilbene also reduced telomerase levels in both cell lines in a dose-dependent manner. Moreover, cMyc, a proposed target of the pterostilbene-mediated inhibition of hTERT, was down-regulated both transcriptionally and posttranscriptionally after treatment. Collectively, these findings highlight a promising use of pterostilbene as a natural, preventive and therapeutic agent against the development and progression of breast cancer.

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Pterostilbene Inhibits Breast Cancer In Vitro Through Mitochondrial Depolarization and Induction of Caspase-Dependent Apoptosis

Cited by 75 publications

References 23 publications

Pterostilbene induces cell cycle arrest and apoptosis in MOLT4 human leukemia cells

Pterostilbene induces cell cycle arrest and apoptosis in MOLT4 human leukemia cells

Anti-proliferative effect of pterostilbene on rat hepatoma cells in culture

Pterostilbene down‐regulates hTERT at physiological concentrations in breast cancer cells: Potentially through the inhibition of cMyc

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