Pterocarpans and triterpenoids from Gueldenstaedtia verna

Chemical constituents from Gueldenstaedtia verna and their anti-inflammatory activity

“…Fraction C (55.7 g) was recrystallized from methanol affording compound 13 (5.2 mg), and the filtrate was then purified by a Sephadex LH-20 column eluting with (CH 2 Cl 2 -MeOH, 1:1) to afford compound 14 (5.0 mg). Compound 8 (32.9 mg) was also obtained from the fraction C, which was described in our earlier paper (Yin et al 2015).…”

“…The inhibitory effect on NO production in RAW 264.7 cells was tested by a previously reported method (Yin et al 2015). Briefly, RAW 264.7 cells were seeded in 96-well plates at a density of 2 × 10 5 cells/well for 24 h, and then stimulated with LPS (1 μg/mL) in the presence or absence of various concentrations of testing compounds and positive control for 24 h. After incubation, an aliquot of the culture supernatants (100 µL) was added to react with a solution of Griess reagent (50 µL, 1:1 mixture of 1% sulfanilamide in 5% H 3 PO 4 and 0.1% N-(1-naphthyl) ethylenediamine dihydrochloride in H 2 O) in the 96-well plate for 20 min at room temperature.…”

Triterpenoids

“…187 The rhamnoside 590, from Gueldenstaedtia verna has a new genin. 190 Pachystelanoside B is a saponin from Pachystela msolo with the new genin 7a-hydroxyprotobassic acid 591. 195 Silenorubicosides E-I are saponins from Silene rubicunda with the new genins 592 (E, F and G), 593 (H) and 594 (I).…”