Psychrobacter piscatorii sp. nov., a psychrotolerant bacterium exhibiting high catalase activity isolated from an oxidative environment

Yumoto, Isao; Hirota, Kikue; Kimoto, Hideyuki; Nodasaka, Yoshinobu; Matsuyama, Hidetoshi; Yoshimune, Kazuaki

doi:10.1099/ijs.0.010959-0

Cited by 27 publications

(13 citation statements)

References 25 publications

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“…Three novel species of psychrotolerant H 2 O 2 -resistant bacteria, namely, V . rumoiensis [12]–[17], Exiguobacterium oxidotolerans [18]–[20] and Psychrobacter piscatorii [21]–[23], have been isolated and their catalases have been studied. Among these isolates, E. oxidotolerans T-2-2 T is the only Gram-positive bacterium and it exhibits high catalase activity in the extracellular fraction.…”

Section: Introductionmentioning

confidence: 99%

Growth-Dependent Catalase Localization in Exiguobacterium oxidotolerans T-2-2T Reflected by Catalase Activity of Cells

et al. 2013

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A psychrotolerant and H2O2-resistant bacterium, Exiguobacterium oxidotolerans T-2-2T, exhibits extraordinary H2O2 resistance and produces catalase not only intracellularly but also extracellularly. The intracellular and extracellular catalases exhibited the same enzymatic characteristics, that is, they exhibited the temperature-dependent activity characteristic of a cold-adapted enzyme, their heat stabilities were similar to those of mesophilic enzymes and very high catalytic intensity. In addition, catalase gene analysis indicated that the bacterium possessed the sole clade 1 catalase gene corresponding to intracellular catalase. Hence, intracellular catalase is secreted into the extracellular space. In addition to intracellular and extracellular catalases, the inner circumference of the cells showed the localization of catalase in the mid-stationary growth phase, which was observed by immunoelectron microscopy using an antibody against the intracellular catalase of the strain. The cells demonstrated higher catalase activity in the mid-stationary growth phase than in the exponential growth phase. The catalase localized in the inner circumference can be dissociated by treatment with Tween 60. Thus, the localized catalase is not tightly bound to the inner circumference of the cells and may play a role in the oxidative defense of the cells under low metabolic state.

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Section: Introductionmentioning

confidence: 99%

Growth-Dependent Catalase Localization in Exiguobacterium oxidotolerans T-2-2T Reflected by Catalase Activity of Cells

et al. 2013

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“…Most of the species described so far have been isolated from cold environments, including Arctic and Antarctic sea ice, water, soils and sediments (Bowman et al, 1996(Bowman et al, , 1997Yumoto et al, 2003;Romanenko et al, 2004;Shivaji et al, 2004;Bakermans et al, 2006). Less frequently, new species have been isolated from temperate marine environments (Yoon et al, 2005b;Yumoto et al, 2010) and other sources such as pigeon feces, food products, lung tissue and human blood (Vela et al, 2003;Yoon et al, 2003Yoon et al, , 2005aYassin & Busse, 2009;Wirth et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Molecular analysis of the diversity of genusPsychrobacterpresent within a temperate estuary

Azevedo

Correia

Henriques

2013

FEMS Microbiol Ecol

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Many members of the genus Psychrobacter are endemic in extremely cold and saline environments and the genus has been described as only marginally successful in warmer habitats. In a previous study the Psychrobacter genus was, unexpectedly, the most frequently isolated bacterial genus from the sea-surface microlayer (SML) and the underlying water (UW) of a temperate estuary (Ria de Aveiro, Portugal). Here we analysed the diversity in Psychrobacter populations inhabiting this estuary. Samples were collected at three dates and three locations from sea-SML and UW. Isolated Psychrobacter strains were well-adapted to temperatures and salt concentrations above the ones described as optimal for most members of this genus. Hydrocarbon-degrading potential was not confirmed for these strains. We developed and optimized a reliable and specific denaturing gradient gel electrophoresis (DGGE)-based method for the analysis of Psychrobacter populations in aquatic systems. DGGE profiles inferred that Psychrobacter populations were very stable in the estuary, a strong indication for the presence of well-adapted phylotypes. The analysis of genus-specific clone libraries revealed a surprisingly high diversity among Psychrobacter in Ria de Aveiro. Results indicated that novel species were probably cultivated. Significant differences between sea-SML and UW Psychrobacter communities were revealed. Observed diversity trends may be related to environmental factors such as salinity and/or anthropogenic pressures such as contamination with hydrocarbons.

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“…EKTA is a clade 1 catalase. We have isolated the third strain, Psychrobacter piscatorii T-3-2 T , whose cell extract exhibits 12,000 U·mg protein −1 , from the same place where strain T-2-2 T was isolated [21]. In addition to strain T-3-2 T , we have isolated P. piscatorii strain T-3 found to have similar characteristics to strain T-3-2 T , which exhibited a higher activity than strain T-3-2 T .…”

Section: Introductionmentioning

confidence: 99%

Characterization of Catalase from Psychrotolerant Psychrobacter piscatorii T-3 Exhibiting High Catalase Activity

Kimoto

Yoshimune

Matsuyma

et al. 2012

IJMS

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A psychrotolerant bacterium, strain T-3 (identified as Psychrobacter piscatorii), that exhibited an extraordinarily high catalase activity was isolated from the drain pool of a plant that uses H2O2 as a bleaching agent. Its cell extract exhibited a catalase activity (19,700 U·mg protein−1) that was higher than that of Micrococcus luteus used for industrial catalase production. Catalase was approximately 10% of the total proteins in the cell extract of the strain. The catalase (PktA) was purified homogeneously by only two purification steps, anion exchange and hydrophobic chromatographies. The purified catalase exhibited higher catalytic efficiency and higher sensitivity of activity at high temperatures than M. luteus catalase. The deduced amino acid sequence showed the highest homology with catalase of Psycrobacter cryohalolentis, a psychrotolelant bacterium obtained from Siberian permafrost. These findings suggest that the characteristics of the PktA molecule reflected the taxonomic relationship of the isolate as well as the environmental conditions (low temperatures and high concentrations of H2O2) under which the bacterium survives. Strain T-3 efficiently produces a catalase (PktA) at a higher rate than Exiguobacterium oxidotolerans, which produces a very strong activity of catalase (EktA) at a moderate rate, in order to adapt to high concentration of H2O2.

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Psychrobacter piscatorii sp. nov., a psychrotolerant bacterium exhibiting high catalase activity isolated from an oxidative environment

Cited by 27 publications

References 25 publications

Growth-Dependent Catalase Localization in Exiguobacterium oxidotolerans T-2-2T Reflected by Catalase Activity of Cells

Growth-Dependent Catalase Localization in Exiguobacterium oxidotolerans T-2-2T Reflected by Catalase Activity of Cells

Molecular analysis of the diversity of genusPsychrobacterpresent within a temperate estuary

Characterization of Catalase from Psychrotolerant Psychrobacter piscatorii T-3 Exhibiting High Catalase Activity

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