Characterization of Catalase from Psychrotolerant Psychrobacter piscatorii T-3 Exhibiting High Catalase Activity

Abstract

A psychrotolerant bacterium, strain T-3 (identified as Psychrobacter piscatorii), that exhibited an extraordinarily high catalase activity was isolated from the drain pool of a plant that uses H2O2 as a bleaching agent. Its cell extract exhibited a catalase activity (19,700 U·mg protein−1) that was higher than that of Micrococcus luteus used for industrial catalase production. Catalase was approximately 10% of the total proteins in the cell extract of the strain. The catalase (PktA) was purified homogeneously by only two purification steps, anion exchange and hydrophobic chromatographies. The purified catalase exhibited higher catalytic efficiency and higher sensitivity of activity at high temperatures than M. luteus catalase. The deduced amino acid sequence showed the highest homology with catalase of Psycrobacter cryohalolentis, a psychrotolelant bacterium obtained from Siberian permafrost. These findings suggest that the characteristics of the PktA molecule reflected the taxonomic relationship of the isolate as well as the environmental conditions (low temperatures and high concentrations of H2O2) under which the bacterium survives. Strain T-3 efficiently produces a catalase (PktA) at a higher rate than Exiguobacterium oxidotolerans, which produces a very strong activity of catalase (EktA) at a moderate rate, in order to adapt to high concentration of H2O2.

Growth-Dependent Catalase Localization in Exiguobacterium oxidotolerans T-2-2T Reflected by Catalase Activity of Cells

“…Three novel species of psychrotolerant H 2 O 2 -resistant bacteria, namely, V . rumoiensis [12]–[17], Exiguobacterium oxidotolerans [18]–[20] and Psychrobacter piscatorii [21]–[23], have been isolated and their catalases have been studied. Among these isolates, E. oxidotolerans T-2-2 T is the only Gram-positive bacterium and it exhibits high catalase activity in the extracellular fraction.…”

A New Homo-Hexamer Mn-Containing Catalase from Geobacillus sp. WCH70

“…The thermostability of GWC is better than most heme catalases due to its four α-helix bundle robust structure. The half-life of purified GWC is 7.5 h at 80 • C. This result is better than most heme catalases such as those from Serratia marcescens SYBC08 (90 min, 60%, 70 • C) [33], Psychrobacter piscatorii T-3 (15 min, 50%, 60 • C) [34], Vibrio salmonicida (10 min, 0%, 60 • C) [35], Vibrio rumoiensis S-1 T (15 min, 0%, 60 • C) [36], Halomonas sp. SK1 (30 min, 0%, 55 • C) [37], Psychrobacter piscatorii (15 min, 20%, 65 • C) [38], and Geobacillus sp.…”

Manipulation of culture conditions for extensive extracellular catalase production by Exiguobacterium oxidotolerans T-2-2T

“…Other hydrogen-peroxide-tolerant bacteria that exhibit high catalase activity could be candidates for low-cost catalase production. Indeed, we have isolated other hydrogenperoxide-tolerant bacteria, namely, Vibrio rumoiensis S-1 T (Yumoto et al 1998(Yumoto et al , 1999(Yumoto et al , 2000 and Psychrobacter piscatorii T-3 (Kimoto et al 2008(Kimoto et al , 2012. Although their intracellular-catalase-producing abilities are very high, the extracellular catalase productivity of E. oxidotolerans T-2-2 T is higher than that of intracellular catalase (Table 1).…”

“…We have isolated H 2 O 2 -tolerant microorganisms from the wastewater of a fish processing plant that uses H 2 O 2 as the bleaching agent for fish eggs (Yumoto et al 1998(Yumoto et al , 1999(Yumoto et al , 2004(Yumoto et al , 2010Kimoto et al 2012) and have characterized the catalases from these isolates (Yumoto et al 2000;Hara et al 2007;Kimoto et al 2012). The high catalase activity detected in the supernatant of the spent culture medium will facilitate purification of the catalase by eliminating the need for a the cell disruption step to obtain intracellular catalase and a large amount of intracellular background proteins.…”