Psychrilyobacter atlanticus gen. nov., sp. nov., a marine member of the phylum Fusobacteria that produces H2 and degrades nitramine explosives under low temperature conditions

Zhao, Jihe; Manno, Dominic; Hawari, Jalal

doi:10.1099/ijs.0.65263-0

Cited by 57 publications

(42 citation statements)

References 28 publications

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“…The 16S rRNA sequence of these anaerobically grown bacteria revealed the presence of two independent isolates very closely related to Psychrilyobacter atlanticus, thus far the only reported representative of this genus. Psychrilyobacter was fully described as a new genus in 2009 (Zhao et al 2009) after isolation from marine sediment 215 m deep in the Atlantic ocean 50 nautical miles from Halifax harbour, Canada. It is a Gram-negative, psychrotrophic, salt-requiring, short rod and an obligate anaerobe.…”

Section: Discussionmentioning

confidence: 99%

A survey of culturable aerobic and anaerobic marine bacteria in de novo biofilm formation on natural substrates in St. Andrews Bay, Scotland

Finnegan

Garcia-Melgares

Gmerek

et al. 2011

Antonie van Leeuwenhoek

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This study reports a novel study of marine biofilm formation comprising aerobic and anaerobic bacteria. Samples of quartz and feldspar, minerals commonly found on the earth, were suspended 5 m deep in the North Sea off the east coast of St. Andrews, Scotland for 5 weeks. The assemblage of organisms attached to these stones was cultivated under aerobic and anaerobic conditions in the laboratory. Bacteria isolated on Marine Agar 2216 were all Gram-negative and identified to genus level by sequencing the gene encoding 16S rRNA. Colwellia, Maribacter, Pseudoaltermonas and Shewanella were observed in aerobically-grown cultures while Vibrio was found to be present in both aerobic and anaerobic cultures. The obligate anaerobic bacterium Psychrilyobacter atlanticus, a recently defined genus, was identified as a close relative of isolates grown anaerobically. The results provide valuable information as to the main players that attach and form de novo biofilms on common minerals in sea water.

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Section: Discussionmentioning

confidence: 99%

A survey of culturable aerobic and anaerobic marine bacteria in de novo biofilm formation on natural substrates in St. Andrews Bay, Scotland

Finnegan

Garcia-Melgares

Gmerek

et al. 2011

Antonie van Leeuwenhoek

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“…This species was one of the most abundant species in most of the analyzed sites. Psychrilyobacter produces H2 and acetate and has the ability to degrade hexahydro-1,3,5-trinitro-1,3,5-triazine and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine, two nitramine explosives (Zhao et al, 2009). Tepidibacter was isolated from an oil field in China (Tan et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Egypt's Red Sea coast: phylogenetic analysis of cultured microbial consortia in industrialized sites

Mustafa

Abd‐Elgawad²,

Abdel-Haleem

et al. 2014

Front. Microbiol.

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The Red Sea possesses a unique geography, and its shores are rich in mangrove, macro-algal and coral reef ecosystems. Various sources of pollution affect Red Sea biota, including microbial life. We assessed the effects of industrialization on microbes along the Egyptian Red Sea coast at eight coastal sites and two lakes. The bacterial communities of sediment samples were analyzed using bacterial 16S rDNA pyrosequencing of V6-V4 hypervariable regions. The taxonomic assignment of 131,402 significant reads to major bacterial taxa revealed five main bacterial phyla dominating the sampled sites: Proteobacteria (68%), Firmicutes (13%), Fusobacteria (12%), Bacteriodetes (6%), and Spirochetes (0.03%). Further analysis revealed distinct bacterial consortia that primarily included (1) marine Vibrio spp.—suggesting a “marine Vibrio phenomenon”; (2) potential human pathogens; and (3) oil-degrading bacteria. We discuss two divergent microbial consortia that were sampled from Solar Lake West near Taba/Eilat and Saline Lake in Ras Muhammad; these consortia contained the highest abundance of human pathogens and no pathogens, respectively. Our results draw attention to the effects of industrialization on the Red Sea and suggest the need for further analysis to overcome the hazardous effects observed at the impacted sites.

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“…P. atlanticus and C. fermentans are typical primary fermenting bacteria (Bachmann, 1955;Zhao et al, 2009), whereas P. modestum is known to be a secondary fermenting organism (Schink, 2006). Relatives of P. atlanticus and Propionigenium were already 13 C labeled after 12 h. The Cytophagarelated bacterium showed enhanced 13 C labeling after 3 days.…”

Section: Total Cell Counts and Community Analysismentioning

confidence: 95%

“…Both have previously been found in marine sediments (Bachmann, 1955;Zhao et al, 2009). P. atlanticus is a primary fermenting bacterium that produces acetate and hydrogen as main fermentation products (Zhao et al, 2009).…”

Section: Process Assessmentmentioning

confidence: 99%

Degradation of cyanobacterial biomass in anoxic tidal-flat sediments: a microcosm study of metabolic processes and community changes

2011

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To follow the anaerobic degradation of organic matter in tidal-flat sediments, a stimulation experiment with 13 C-labeled Spirulina biomass (130 mg per 21 g sediment slurry) was conducted over a period of 24 days. A combination of microcalorimetry to record process kinetics, chemical analyses of fermentation products and RNA-based stable-isotope probing (SIP) to follow community changes was applied. Different degradation phases could be identified by microcalorimetry: Within 2 days, heat output reached its maximum (55 lW), while primary fermentation products were formed (in lmol) as follows: acetate 440, ethanol 195, butyrate 128, propionate 112, H 2 127 and smaller amounts of valerate, propanol and butanol. Sulfate was depleted within 7 days. Thereafter, methanogenesis was observed and secondary fermentation proceeded. H 2 and alcohols disappeared completely, whereas fatty acids decreased in concentration. Three main degraders were identified by RNA-based SIP and denaturant gradient gel electrophoresis. After 12 h, two phylotypes clearly enriched in 13 C: (i) Psychrilyobacter atlanticus, a fermenter known to produce hydrogen and acetate and (ii) bacteria distantly related to Propionigenium. A Cytophaga-related bacterium was highly abundant after day 3. Sulfate reduction appeared to be performed by incompletely oxidizing species, as only sulfate-reducing bacteria related to Desulfovibrio were labeled as long as sulfate was available.

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Psychrilyobacter atlanticus gen. nov., sp. nov., a marine member of the phylum Fusobacteria that produces H2 and degrades nitramine explosives under low temperature conditions

Cited by 57 publications

References 28 publications

A survey of culturable aerobic and anaerobic marine bacteria in de novo biofilm formation on natural substrates in St. Andrews Bay, Scotland

A survey of culturable aerobic and anaerobic marine bacteria in de novo biofilm formation on natural substrates in St. Andrews Bay, Scotland

Egypt's Red Sea coast: phylogenetic analysis of cultured microbial consortia in industrialized sites

Degradation of cyanobacterial biomass in anoxic tidal-flat sediments: a microcosm study of metabolic processes and community changes

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