A survey of culturable aerobic and anaerobic marine bacteria in de novo biofilm formation on natural substrates in St. Andrews Bay, Scotland

Abstract

This study reports a novel study of marine biofilm formation comprising aerobic and anaerobic bacteria. Samples of quartz and feldspar, minerals commonly found on the earth, were suspended 5 m deep in the North Sea off the east coast of St. Andrews, Scotland for 5 weeks. The assemblage of organisms attached to these stones was cultivated under aerobic and anaerobic conditions in the laboratory. Bacteria isolated on Marine Agar 2216 were all Gram-negative and identified to genus level by sequencing the gene encoding 16S rRNA. Colwellia, Maribacter, Pseudoaltermonas and Shewanella were observed in aerobically-grown cultures while Vibrio was found to be present in both aerobic and anaerobic cultures. The obligate anaerobic bacterium Psychrilyobacter atlanticus, a recently defined genus, was identified as a close relative of isolates grown anaerobically. The results provide valuable information as to the main players that attach and form de novo biofilms on common minerals in sea water.

Phylogenetic identification of marine bacteria isolated from deep-sea sediments of the eastern South Atlantic Ocean

“…This is not unexpected considering that bacteria from this phylogenetic group are among the most known and readily cultivable microorganisms from the marine environment (Fuhrman and Hagström 2008), and this result is in agreement with reports from other studies on diverse marine environments (; Kobayashi et al 2008; Ettoumia et al 2011; Finnegan et al 2011). In the culture–independent study of Schauer et al (2010) conducted at the Angola, Guinea and Cape Basins, Gammaproteobacteria was also the dominant group, although most of the phylotypes identified belonged to not yet cultivated species.…”

Response of bacteria and phytoplankton from a subtropical front location Southern Ocean to micronutrient amendments ex-situ.

“…The bacteria from this phylogenetic group are among the most known and readily cultivable microorganisms from the marine environment (Fuhrman and Hagström, 2008), as noted in this and many other studies (Yanagibayashi et al, 1999;Kobayashi et al, 2008;Ettoumia et al, 2010;Finnegan et al, 2011). The genus Pseudoalteromonas has often been found in close association with marine eukaryotic hosts (Hölmstrom and Kjelleberg, 1999) and known for producing agarolytic enzymes involved in the algal tissue degradation (Ivanova et al, 2002).…”

Bioaccumulation of Vanadium by Vanadium-Resistant Bacteria Isolated from the Intestine of Ascidia sydneiensis samea

“…In oyster, V. tasmaniensis expresses coper related gene to fight againts antibactericidal effects released by hemocyte (Vanhove et al 2016), while in ascidian they have been associated with biofilm communities . Strains S-RA-6 and S-RA-7 were the most abundant strains of the genus Shewanella, and corresponded to S. kaereitica (99.87 ± 0.17%) and S. pasifica (99.67%), which are commonly found in seawater, deep-sea sediment, and as biofilm formations on natural substrates Finnegan et al 2011). They occur in very low numbers compared to other predominate intestinal bacteria;…”

“…RA-7 were the most abundant strains of the genus Shewanella, and corresponded to S. kaereitica (99.87 ± 0.17%) and S. pasifica (99.67%), which are commonly found in seawater, deep-sea sediment, and as biofilm formations on natural substratesFinnegan et al 2011). They occur in very low numbers compared to other predominate intestinal bacteria; Vibrio and Shewanella are known to inhabit the gut of Ciona intestinalis with 1.5% and 4% OTU, respectively(Dishaw et al 2014), although the data for A. sydneiensis samea is not available yet.…”