Psidium guajava L. and Psidium brownianum Mart ex DC.: Chemical composition and anti – Candida effect in association with fluconazole

Morais-Braga, Maria Flaviana Bezerra; Sales, Débora Lima; Carneiro, Joara Nályda Pereira; Machado, Antônio Júdson Targino; Santos, Antônia Thassya Lucas dos; Freitas, Maria Audilene de; Martins, Gioconda Morais de Andrade Bezerra; Leite, Nádghia Figueiredo; Matos, Yedda Maria Lobo Soares de; Tintino, Saulo Relison; Souza, Djair S.L.; Menezes, Irwin Rose Alencar de; Ribeiro-Filho, Jaime; Costa, José G.M.; Coutinho, Henrique Douglas Melo

doi:10.1016/j.micpath.2016.04.013

Cited by 67 publications

(52 citation statements)

References 33 publications

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“…ex DC. And Psidium guajava L., the former having medicinal properties and is used to combat infections caused by fungi of the genus Candida [6,27].…”

Section: Discussionmentioning

confidence: 99%

“…The plates were incubated at 37 • C for 24 h and subsequently read on a 630 nm wavelength ELISA spectrophotometer (Thermoplate ® ). All assays were performed in triplicate and the results obtained were used to construct the cell viability curve and the IC 50 of essential oil and fluconazole [6,32]. MIC was defined as the lowest concentration able to reduce the fungal growth curve.…”

Section: Determination Of Minimum Inhibitory Concentration (Mic)mentioning

confidence: 99%

“…Yeasts of the genus Candida are found as commensals in the human organism, however, because of factors that disturb the dynamics of the host can favor the growth of these fungi, from superficial infections to systemic infections [5]. Among the yeasts of this genus, Candida albicans stands out as the main cause of infections, being a species with great ability to change its morphology, a necessary factor for its virulence and pathogenicity [6,7].…”

Section: Introductionmentioning

confidence: 99%

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Antibiotic Potential and Chemical Composition of the Essential Oil of Piper caldense C. DC. (Piperaceae)

et al. 2020

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Infections by multiresistant microorganisms have led to a continuous investigation of substances acting as modifiers of this resistance. By following this approach, the chemical composition of the essential oil from Piper caldense leaf and its antimicrobial potential were investigated. The antimicrobial activity was determined by broth microdilution method providing values for minimum inhibitory concentration (MIC), IC50, and minimum fungicidal concentration (MFC). The essential oil was tested as a modulator for several antibiotics, and its effect on the morphology of Candida albicans (CA) strains was also investigated. The chemical characterization revealed an oil composed mainly of sesquiterpenes. Among them are caryophyllene oxide (13.9%), spathulenol (9.1%), δ-cadinene (7.6%) and bicyclogermacrene (6.7%) with the highest concentrations. The essential oil showed very low activity against the strains of CA with the lowest values for IC50 and MFC of 1790 μg/mL and 8192 μg/mL, respectively. The essential oil modulated the activity of fluconazole against CA URM 4387 strain, which was demonstrated by the lower IC50 obtained, 2.7 μg/mL, whereas fluconazole itself presented an IC50 of 7.76 μg/mL. No modulating effect was observed in the MFC bioassays. The effect on fungal morphology was observed for both CA INCQS 40006 and URM 4387 strains. The hyphae projection was completely inhibited at 4096 μg/mL and 2048 μg/mL, respectively. Thus, the oil has potential as an adjuvant in antimicrobial formulations.

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“…ex DC. And Psidium guajava L., the former having medicinal properties and is used to combat infections caused by fungi of the genus Candida [6,27].…”

Section: Discussionmentioning

confidence: 99%

Section: Determination Of Minimum Inhibitory Concentration (Mic)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antibiotic Potential and Chemical Composition of the Essential Oil of Piper caldense C. DC. (Piperaceae)

et al. 2020

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“…Microorganisms of the genus Candida have acquired resistance to a variety of antifungal drugs, leading to poor treatment and increasing disease severity. Hence, studies on new pharmacologically active compounds for the treatment of fungal conditions are warranted (Morais-Braga et al, 2016). …”

Section: Introductionmentioning

confidence: 99%

Biosynthesis of silver nanoparticles using Caesalpinia ferrea (Tul.) Martius extract: physicochemical characterization, antifungal activity and cytotoxicity

Soares

Corrêa

Stroppa

et al. 2018

PeerJ

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BackgroundGreen synthesis is an ecological technique for the production of well characterized metallic nanoparticles using plants. This study investigated the synthesis of silver nanoparticles (AgNPs) using a Caesalpinia ferrea seed extract as a reducing agent.MethodsThe formation of AgNPs was identified by instrumental analysis, including ultraviolet–visible (UV–Vis) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD) of the AgNPs, and surface-enhanced Raman scattering (SERS) spectra of rhodamine-6G (R6G). We studied the physicochemical characterization of AgNPs, evaluated them as an antifungal agent against Candida albicans, Candida kruzei, Candida glabrata and Candida guilliermondii, and estimated their minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values. Lastly, this study evaluated the cytotoxicity of the AgNPs in murine L929 fibroblasts cells using an MTT assay.ResultsThe UV–Vis spectroscopy, SERS, SEM and XRD results confirmed the rapid formation of spheroidal 30–50 nm AgNPs. The MIC and MFC values indicated the antifungal potential of AgNPs against most of the fungi studied and high cell viability in murine L929 fibroblasts. In addition, this study demonstrated that C. ferrea seed extracts may be used for the green synthesis of AgNPs at room temperature for the treatment of candidiasis.

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“…In the Cariri region, in addition to the fruit being edible, the leaves are used in traditional medicine to treat diseases and symptoms such as stomach ache and diarrhea, which may be related to Candida infections (Ribeiro et al, 2014;Souza et al, 2014;Macêdo et al, 2016). Fungal infections caused by dermatophytes and yeasts of Candida spp., are a serious health problem in immunocompromised patients in particular and are aggravated by the increase Manuscript to be reviewed in clinical resistance to the antifungal agents (Silva et al, 2012;Morais-Braga et al, 2016b). In view of this problem, the interest in the use of vegetable derivatives with therapeutic potential for antifungal action has intensified (Macêdo et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Peer Review #3 of "Effect of seasonality on chemical profile and antifungal activity of essential oil isolated from leaves Psidium salutare (Kunth) O. Berg (v0.2)"

2018

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Medicinal plants play a crucial role in the search for components that are capable of neutralizing the multiple mechanisms of fungal resistance. Psidium salutare (Kunth) O. Berg is a plant native to Brazil used as both food and traditional medicine to treat diseases and symptoms such as stomach ache and diarrhea, whose symptoms could be related to fungal infections from the genus Candida. The objective of this study was to investigate the influence of seasonal variability on the chemical composition of the Psidium salutare essential oil, its antifungal potential and its effect on the Candida albicans morphogenesis. The essential oils were collected in three different seasonal collection periods and isolated by the hydrodistillation process in a modified Clevenger apparatus with identification of the chemical composition determined by gas chromatography coupled to mass spectrometry (GC/MS). The antifungal assays were performed against Candida strains through the broth microdilution method to determine the minimum fungicidal concentration (MFC). Fungal growth was assessed by optical density reading and the Candida albicans dimorphic effect was evaluated by optical microscopy in microculture chambers. The chemical profile of the essential oils identified 40 substances in the different collection periods with γ-terpinene being the predominant constituent. The antifungal activity revealed an action against the C. albicans, C. krusei and C. tropicalis strains with an IC 50 ranging from 345.5 to 2754.2 μg/mL and a MFC higher than 1024 μg/mL. When combined with essential oils at sub-inhibitory concentrations (MIC/16), fluconazole had its potentiated effect, ie a synergistic effect was observed in the combination of fluconazole with P.salutare oil against all Candida strains, however , for C. albicans, its effect was reinforced by the natural product in all the collection periods. The

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Psidium guajava L. and Psidium brownianum Mart ex DC.: Chemical composition and anti – Candida effect in association with fluconazole

Cited by 67 publications

References 33 publications

Antibiotic Potential and Chemical Composition of the Essential Oil of Piper caldense C. DC. (Piperaceae)

Antibiotic Potential and Chemical Composition of the Essential Oil of Piper caldense C. DC. (Piperaceae)

Biosynthesis of silver nanoparticles using Caesalpinia ferrea (Tul.) Martius extract: physicochemical characterization, antifungal activity and cytotoxicity

Peer Review #3 of "Effect of seasonality on chemical profile and antifungal activity of essential oil isolated from leaves Psidium salutare (Kunth) O. Berg (v0.2)"

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