Pseudoxanthomonas winnipegensis sp. nov., derived from human clinical materials and recovered from cystic fibrosis and other patient types in Canada, and emendation of Pseudoxanthomonas spadix Young et al. 2007

Abstract

Twelve isolates recovered from 10 cystic fibrosis/other patient types and a variety of clinical sources, were referred to Canada's National Microbiology Laboratory over 7 years. These were assignable to the genus Pseudoxanthomonas but were unidentifiable to species level. Patients included five males and five females from two geographically separated provinces, ranging in age from 2 months to 84 years. In contrast, most Pseudoxanthomonas species described to date have been derived from water, plants or contaminated soils. By 16S rRNA gene sequencing, the patient strains had ≥99.4 % similarity to each other but only 97.73–98.29 % to their closest relatives, Pseudoxanthomonas spadix or Pseudoxanthomonas helianthi . Bacteria were studied by whole genome sequencing using average nucleotide identity by Blastn, digital DNA–DNA hybridization, average amino acid identity, core genome and single nucleotide variant analyses, MALDI-TOF, biochemical and cellular fatty acid analyses, and by antimicrobial susceptibility testing. Bacterial structures were assessed using scanning and transmission electron microscopy. Strains were strict aerobes, yellowish-pigmented, oxidative, non-motile, Gram-stain-negative bacilli and generally unable to reduce nitrate. Strains were susceptible to most of the antibiotics tested; some resistance was observed towards carbapenems, several cephems and uniformly to nitrofurantoin. The single taxon group observed by 16S rRNA gene sequencing was supported by whole genome sequencing; genomes ranged in size from 4.36 to 4.73 Mb and had an average G+C content of 69.12 mol%. Based on this study we propose the name Pseudoxanthomonas winnipegensis sp. nov. for this cluster. Pseudoxanthomonas spadix DSM 18855T, acquired for this study, was found to be non-motile phenotypically and by electron microscopy; we therefore propose the emendation of Pseudoxanthomonas spadix Young et al. 2007 to document that observation.

Notification List of Bacterial Strains Made Available by the United Kingdom National Collection of Type Cultures in 2021

“…NCTC 14457 to NCTC 14465 are eight strains of Staphylococcus aureus that carry the methicillin resistance gene mecC isolated from human clinical sources ( 8 ). These strains were isolated originally in a six-hospital surveillance study in the United Kingdom and date from a little over a year after the mecC gene was first discovered in an isolate recovered from a milk bulk tank and consequently described ( 9 ).…”

Isolation and Characterization of the Lytic Pseudoxanthomonas kaohsiungensi Phage PW916

“…Moreover, a recent correlational study characterizing the microbiomes of both oral cavities and tonsils in children with tonsillar hypertrophy suggested that Pseudoxanthomonas might be linked to tonsillar hypertrophy [5]. Twelve isolates of the Pseudoxanthomonas genus recovered from 10 patients located in two geographically separated Canadian provinces were referred to the Special Bacteriology unit at Canada's National Microbiology Laboratory over 7 years, and some level of resistance was observed against the antibiotic classes of carbapenems and nitrofurantoin and the antibiotic group of cephems [6]. Furthermore, some Pseudoxanthomonas spp.…”