Occurrence and prevalence of antibiotic resistance genes and pathogens in an industrial park wastewater treatment plant

Wu, Yongyi; Gong, Zhourui; Wang, Shuijing; Song, Liyan

doi:10.1016/j.scitotenv.2023.163278

Cited by 14 publications

(3 citation statements)

References 67 publications

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“…WWTPs are densely populated with highly diverse communities of bacteria and can serve as repositories for the spread of ARGs (Szczepanowski et al 2009 , Rizzo et al 2013 , Karkman et al 2018 , Pazda et al 2019 , Wu et al 2023 ). Moreover, the presence subinhibitory concentrations of antibiotics in wastewater can accelerate resistance (Andersson and Hughes 2014 .)…”

Section: Discussionmentioning

confidence: 99%

Examining the taxonomic distribution of tetracycline resistance in a wastewater plant

Ochman,

Quandt,

Gottell

et al. 2024

Sustainable Microbiology

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Microbial communities serve as reservoirs of antibiotic resistance genes (ARGs) and facilitate the dissemination of these genes to bacteria that infect humans. Relatively little is known about the taxonomic distribution of bacteria harboring ARGs in these reservoirs and the avenues of transmission due to the technical hurdles associated with characterizing the contents of complex microbial populations and the assignment of genes to particular genomes. Focusing on the array of tetracycline resistance (Tcr) genes in the primary and secondary phases of wastewater treatment, 17 of the 22 assayed Tcr genes were detected in at least one sample. We then applied epicPCR to link tetracycline resistance genes to specific bacterial hosts. Whereas Tcr genes tend to vary in their distributions among bacterial taxa according to their modes of action, there were numerous instances in which a particular Tcr gene was associated with a host that was distantly related to all other bacteria bearing the same gene, including several hosts not previously identified. Tcr genes are far less host-restricted than previously assumed, indicating that complex microbial communities serve as settings where ARGs are spread among divergent bacterial phyla.

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Section: Discussionmentioning

confidence: 99%

Examining the taxonomic distribution of tetracycline resistance in a wastewater plant

Ochman,

Quandt,

Gottell

et al. 2024

Sustainable Microbiology

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“…The ARDB results showed that in the control group (Group 1), the main antibiotic resistance genes were vanrb [22], tetq [23], bl2b-tem [24], aac3iii, vanug and cml-e7 (Supplemental Figure S1). However, following NAC treatment (Group 2), the abundances of antibiotic resistance genes were significantly changed, and the main genes were macb [25], tsnr [26], norm, bl2be-per, vansb and pbp1b (Supplemental Figure S1).…”

Section: β-Diversity Analysis Of Pig Gut Microbiome Compositionmentioning

confidence: 99%

Metagenomic Sequencing Analysis of the Effects of Acetylcysteine on the Pig Gut Microbiome

Fu,

Tian,

et al. 2023

Microbiology Research

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The gut microbiota is engaged in multiple interactions affecting host health, and gut dysbiosis can lead to many diseases. However, the effects of acetylcysteine (NAC) on the gut microbiome composition in pigs using metagenomic sequencing have not been reported. In this study, we used metagenome sequencing to study the effects of NAC on the pig gut microbiome. Sequencing results showed that microbial diversity was changed after NAC treatment. Antibiotic Resistance Genes Database (ARDB) analysis demonstrated that the main genes modified were macb, tsnr, norm, bl2be-per, vansb and pbp1b in the NAC group. Our data showed that NAC could affect microbial distribution at the phylum, gene and species levels. At the species level, NAC significantly increased the abundances of Megasphaera, Lactobacillus reuteri and Megasphaeraelsdenii and reduced the abundances of Phascolarctobacterium succinatutens, Prevotellacopri and Selenomonasbovis compared with the control group. In addition, Gene Ontology (GO) analysis revealed that in the NAC group, cellular process, metabolic process and single-organism process were the dominant terms. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis demonstrated that RNA transport, MAPK signaling pathway, cell cycle, glycosylphosphatidylinositol (GPI)-anchor biosynthesis and VEGF signaling pathway were the dominant signaling pathways in the NAC group. In conclusion, our results suggest that NAC may modify the piglet gut microbiome composition and these findings might provide a new strategy for maintaining animal and human health in the future.

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“…The environmental dispersal of antibiotic residues via run-off events is often accompanied by the dispersion of ARBs and ARGs from various hotspots [ 13 , 14 , 15 ]. The presence of ARBs/ARGs in WWTP has been found to favor the development of multidrug-resistant bacteria [ 16 ], which may pose a further risk to human health [ 17 ]. However, there is ongoing debate on whether inputs from AMR reservoirs into the environment specifically facilitate the transfer of ARGs and mobile genetic elements (MGE) to other organisms through horizontal gene transfer (HGT).…”

Section: Introductionmentioning

confidence: 99%

Environmentally Relevant Antibiotic Concentrations Exert Stronger Selection Pressure on River Biofilm Resistomes than AMR-Reservoir Effluents

Flores-Vargas,

Bergsveinson,

Korber

2024

Antibiotics

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Freshwater environments are primary receiving systems of wastewater and effluents, which carry low concentrations of antibiotics and antimicrobial-resistant (AMR) bacteria and genes. Aquatic microbial communities are thus exposed to environmentally relevant concentrations of antibiotics (ERCA) that presumably influence the acquisition and spread of environmental AMR. Here, we analyzed ERCA exposure with and without the additional presence of municipal wastewater treatment plant effluent (W) and swine manure run-off (M) on aquatic biofilm resistomes. Microscopic analyses revealed decreased taxonomic diversity and biofilm structural integrity, while metagenomic analysis revealed an increased abundance of resistance, virulence, and mobile element-related genes at the highest ERCA exposure levels, with less notable impacts observed when solely exposed to W or M effluents. Microbial function predictions indicated increased gene abundance associated with energy and cell membrane metabolism and heavy metal resistance under ERCA conditions. In silico predictions of increased resistance mechanisms did not correlate with observed phenotypic resistance patterns when whole communities were exposed to antimicrobial susceptibility testing. This reveals important insight into the complexity of whole-community coordination of physical and genetic responses to selective pressures. Lastly, the environmental AMR risk assessment of metagenomic data revealed a higher risk score for biofilms grown at sub-MIC antibiotic conditions.

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Occurrence and prevalence of antibiotic resistance genes and pathogens in an industrial park wastewater treatment plant

Cited by 14 publications

References 67 publications

Examining the taxonomic distribution of tetracycline resistance in a wastewater plant

Examining the taxonomic distribution of tetracycline resistance in a wastewater plant

Metagenomic Sequencing Analysis of the Effects of Acetylcysteine on the Pig Gut Microbiome

Environmentally Relevant Antibiotic Concentrations Exert Stronger Selection Pressure on River Biofilm Resistomes than AMR-Reservoir Effluents

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