2012
DOI: 10.1007/s00374-012-0665-6
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Pseudomonas spp. isolates with high phosphate-mobilizing potential and root colonization properties from agricultural bulk soils under no-till management

Abstract: Seven phosphate-mobilizing pseudomonads were isolated, identified, and characterized in terms of their biofertilizer potential and root-colonizing properties. Pseudomonas protegens (ex-fluorescens) CHA0 was used for comparative purposes. Four isolates (LF-MB1, LF-P1, LF-P2, and LF-P3) clustered with members of the "Pseudomonas fluorescens complex," whereas the other three (LF-MB2, LF-V1, and LF-V2) clustered with members of the "Pseudomonas putida/Pseudomonas aeruginosa complex." Assays in buffered liquid grow… Show more

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Cited by 36 publications
(26 citation statements)
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“…and Bacillus sp. between solubilisation ability on plate and in liquid culture as mentioned in the previous investigations by Azziz et al [26] and Fernández et al [27]. Although all the strains showed good solubilisation in liquid cultures amended with the different rock phosphates, the amount of the solved P varied with the strain as well as the rock phosphate origin.…”
Section: Discussionsupporting
confidence: 57%
“…and Bacillus sp. between solubilisation ability on plate and in liquid culture as mentioned in the previous investigations by Azziz et al [26] and Fernández et al [27]. Although all the strains showed good solubilisation in liquid cultures amended with the different rock phosphates, the amount of the solved P varied with the strain as well as the rock phosphate origin.…”
Section: Discussionsupporting
confidence: 57%
“…Then, 20 μL was injected into the HPLC instrument (Essentia LC-15C, Japan), equipped with a C-18 column, and run at a flow rate of 1 mL/min using a 90:10 (v/v) methanol:phosphate buffer (10 mmol/L), pH 2.7, as the mobile phase; this process was monitored at 210 nm (Paavilainen and Korpela, 1993; Qin et al, 2010; Fernández et al, 2012). …”
Section: Methodsmentioning
confidence: 99%
“…Proteolytic activities were detected as a clear zone around the colonies . The relative extracellular protease and phospholipase activities were calculated as (halo diameter/colony diameter) ×100 according to Fernández et al .…”
Section: Methodsmentioning
confidence: 99%
“…activities were calculated as (halo diameter/colony diameter) Â100 according to Fern andez et al [26]. Siderophore production was estimated on cromo azurol S (CAS) agar plates [27].…”
Section: Identification Of Selected Strainsmentioning
confidence: 99%