Pseudomonas sp. LZ-Q continuously degrades phenanthrene under hypersaline and hyperalkaline condition in a membrane bioreactor system

Abstract

Graphical AbstractAbstractPhenanthrene is one of the most recalcitrant components of crude oil-contaminated wastewater. An efficient phenanthrene-degrading bacterium Pseudomonas sp. strain named LZ-Q was isolated from oil-contaminated soil near the sewage outlet of a petrochemical company. Pseudomonas sp. LZ-Q is able to degrade 1000 mg/L phenanthrene in Bushnell-Hass mineral salt medium. It also degrades other polycyclic aromatic hydrocarbons such as naphthalene, anthracene, pyrene, petrol, and diesel at broad ranges of salinities of 5 g/L to 75 g/L, pHs of 5.0–10.0, and temperatures of 10–42 °C. Therefore, Pseudomonas sp. LZ-Q could be a good candidate for remediation of polycyclic aromatic hydrocarbon (PAH)-contaminated wastewater. A membrane bioreactor (MBR) was applied to investigate the remediation ability of the strain LZ-Q. Wastewater containing phenanthrene with pH of 8, salinity of 35 g/L, and COD of 500 mg/L was continuously added to the system (HRT = 3 h). Results showed that Pseudomonas sp. LZ-Q is capable of degrading 96% of 20 mg/L phenanthrene and 94% of 500 mg/L COD for 60 days in a continuous mode. These results showed that the MBR system with strain LZ-Q might be a good approach for PAHs’ remediation in industrial wastewaters.

The naphthalene catabolic protein NahG plays a key role in hexavalent chromium reduction in Pseudomonas brassicacearum LZ-4

“…High performance liquid chromatography (HPLC; Agilent 1260 Infinity) was used to verify that NahG is involved in the conversion of salicylate to catechol 54 . The metabolites were separated with an Eclipse Plus C18 (4.6 mm × 250 mm).…”

Proteogenomic Characterization of Pseudomonas veronii SM-20 Growing on Phenanthrene as Only Carbon and Energy Source

“…Ultimately, after one month of cultivation, the fold increase in biomass was 2.5 times in terms of protein content (Figure 3). Several Pseudomonas strains have previously been described as capable of degrading PAHs such as PHE [44,45] or naphthalene [12]. Depending on the strain and the initial PHE concentrations, previous studies have reported biodegradation times ranging from two to fifteen days in liquid cultures [46] and from forty to sixty days in soil or reactor systems [44][45][46][47].…”

“…Depending on the strain and the initial PHE concentrations, previous studies have reported biodegradation times ranging from two to fifteen days in liquid cultures [46] and from forty to sixty days in soil or reactor systems [44][45][46][47]. Additionally, concerning biomass growth, Pseudomonas strain LZ-Q and P. stutzeri ZP2 achieved a 10-fold increase in biomass production [44,48] in the presence of 1000 and 250 mg/L of PHE, respectively. On the other hand, strains of P. pseudoalcaligenes Several Pseudomonas strains have previously been described as capable of degrading PAHs such as PHE [44,45] or naphthalene [12].…”

Phenotypic and Genomic Characterization of Pseudomonas wuhanensis sp. nov., a Novel Species with Promising Features as a Potential Plant Growth-Promoting and Biocontrol Agent

“…Pseudomonas is known for its metabolic versatility and capacity to produce abundant secondary metabolites [6,12]. They can utilize various chemical compounds as sources of carbon, nitrogen, or phosphorus, making them interesting microorganisms for processes such as bioremediation and biotransformation [2,13]. For example, Pseudomonas mediteranea S58 can produce siderophores, amylase, and protease, solubilize organic phosphorus, and show strong antagonism against a variety of plant pathogenic fungi and bacteria [14].…”