Pseudomonas pohangensis sp. nov., isolated from seashore sand in Korea

Abstract

A novel bacterium, designated strain H3-R18 T , was isolated from seashore sand collected from Homi cape, Pohang city, Korea. Cells were Gram-negative, aerobic, non-motile, cream-coloured, mesophilic and slightly halotolerant. 16S rRNA gene sequence analysis indicated that the organism was a member of the genus Pseudomonas, but the sequence showed ¡96?3 % sequence similarity to that of the type strains of all recognized Pseudomonas species. Highest sequence similarities were to Pseudomonas brenneri CFML 97-391 T (96?3 %) and Pseudomonas migulae CIP 105470 T (96?3 %). The major fatty acids were summed feature 3 and C 16 : 0 , with lesser amounts of C 12 : 0 , C 12 : 0 3-OH, C 18 : 1 v7c and C 14 : 0 . The major isoprenoid quinone was Q-9. The DNA G+C content was 64?0 mol%. The phylogenetic, phenotypic and genetic properties of strain H3-R18 T suggest that it represents a novel species, for which the name Pseudomonas pohangensis sp. nov. is proposed. The type strain is H3-R18 T (=KACC 11517 T =DSM 17875 T ).

Pseudomonas hussainii sp. nov., isolated from droppings of a seashore bird, and emended descriptions of Pseudomonas pohangensis, Pseudomonas benzenivorans and Pseudomonas segetis

“…Both strains contained ubiquinone 9 (Q-9) as the major respiratory quinone, similar to quinone data reported previously from species of the genus Pseudomonas (Liu et al, 2013;Lin et al, 2013a, b;Feng et al, 2012;Pascual et al, 2012;Weon et al, 2006;Hauser et al, 2004).…”

“…Members of the genus Pseudomonas are ubiquitous in distribution, and have been isolated from various sources such as water (Liu et al, 2013;Pascual et al, 2012;Lang et al, 2010;Hauser et al, 2004), soil (Lin et al, 2013a;Feng et al, 2012;Park et al, 2006), seashore sand (Weon et al, 2006) and food-waste compost (Lin et al, 2013b). The nutritional studies of Stanier et al (1966) on Pseudomonas provided baseline assistance to the phenotypic characterization of representative isolates.…”

“…Pale-yellow colonies of strains CC-AMH-11 T and CC-AMHZ-5 were isolated, purified and preserved in nutrient broth (NB; Himedia) supplemented with 20 % (v/v) glycerol at 280 u C. Taxonomic investigations were carried out according to previously published guidelines (Tindall et al, 2010). Three type strains of the genus Pseudomonas [Pseudomonas pohangensis DSM 17875 T (Weon et al, 2006), P. benzenivorans DSM 8628 T (Lang et al, 2010) and P. segetis KCTC 12331 T (Park et al, 2006)] were used for direct comparative analysis. All five strains were cultured by using nutrient agar (NA; Himedia) or NB for 48 h at 30 u C, unless specified otherwise.…”

“…Emended description of Pseudomonas pohangensis Weon et al 2006 The description is as given by Weon et al (2006) with the following amendments. Phosphatidylethanolamine is the predominant polar lipid.…”

Blue pigment in fresh cheese produced by Pseudomonas fluorescens

“…Isolates were briefly grown on BHI agar plates at 32 C for 18 h. Bacterial cultures were embedded in 1% agarose plugs (SeaKem Gold agarose, Cambrex, Rockland, ME), lysed, washed, and digested Table 1 Differential phenotypic characteristics of Pseudomonas fluorescens and other Pseudomonas species. Table modified from Weon et al (2006). Taxa: 1, strain H3-R18 T ; 2, Pseudomonas aeruginosa (Palleroni, 1984); 3, Pseudomonas alcaligenes (Palleroni, 1984); 4, Pseudomonas alcaliphila (Yumoto et al, 2001); 5, Pseudomonas balearica (Bennasar, Rossell o-Mora, Lalucat, & Moore, 1996); 6, Pseudomonas brenneri (Baïda, Yazourh, Singer, & Izard, 2001); 7, Pseudomonas fluorescens biovar I (Palleroni, 1984); 8, Pseudomonas luteola (Kodama, Odama, Kimura, & Komagata, 1985); 9, Pseudomonas migulae (Verhille et al, 1999); 10, Pseudomonas pachastrellae (Romanenko et al, 2005); 11, Pseudomonas pseudoalcaligenes (Palleroni, 1984); 12, Pseudomonas putida biovars (Palleroni, 1984); 13, Pseudomonas stutzeri (Palleroni, 1984); 14, Pseudomonas fluorescens biovar IV (Cantoni et al, 2001); 15, Pseudomonas fluorescens new biovar (Cantoni et al, 2001); þ, At least 90% of strains positive; À, at least 90% of strains negative; d, 11e89 % of strains positive; ND, not determined.…”