Pseudomonas hussainii sp. nov., isolated from droppings of a seashore bird, and emended descriptions of Pseudomonas pohangensis, Pseudomonas benzenivorans and Pseudomonas segetis

Abstract

Two Gram-staining-negative, aerobic, rod-shaped, non-spore-forming bacterial strains that are motile by a monopolar flagellum, designated CC-AMH-11(T) and CC-AMHZ-5, were isolated from droppings of a seashore bird off the coast of Hualien, Taiwan. The strains showed 99.7% mutual pairwise 16S rRNA gene sequence similarity, while exhibiting <96.2% sequence similarity to strains of other species of the genus Pseudomonas (95.7-95.9% similarity with type species, Pseudomonas aeruginosa LMG 1242T), and formed a distinct co-phyletic lineage in the phylogenetic trees. The common major fatty acids (>5% of the total) were C18 : 1ω7c and/or C18 : 1ω6c (summed feature 8), C16 : 1ω6c and/or C16 : 1ω7c (summed feature 3), C16 : 0 and C12 : 0. Phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylserine, an unidentified lipid and an unidentified phospholipid were detected as common polar lipids. The DNA G+C contents of strains CC-AMH-11(T) and CC-AMHZ-5 were 61.1 and 61.6 mol%, respectively. The common major respiratory quinone was ubiquinone 9 (Q-9), and the predominant polyamine was putrescine. The DNA-DNA hybridization obtained between the two strains was 79.0% (reciprocal value 89.4% using CC-AMHZ-5 DNA as the probe). The very high 16S rRNA gene sequence similarity and DNA-DNA relatedness and the poorly distinguishable phenotypic features witnessed between CC-AMH-11(T) and CC-AMHZ-5 suggested unambiguously that they are two distinct strains of a single genomic species. However, the strains also showed several genotypic and phenotypic characteristics that distinguished them from other closely related species of Pseudomonas. Thus, the strains are proposed to represent a novel species of Pseudomonas, for which the name Pseudomonas hussainii sp. nov. is proposed. The type strain is CC-AMH-11(T) ( = JCM 19513(T) = BCRC 80696(T)); a second strain of the same species is CC-AMHZ-5 ( = JCM 19512 = BCRC 80697). In addition, emended descriptions of the species Pseudomonas pohangensis, Pseudomonas benzenivorans and Pseudomonas segetis are also proposed

Pseudomonas matsuisoli sp. nov., isolated from a soil sample

“…The DNA G+C content of strain CC-MHH0089 T was 63.6±0.5 mol%, which is within the range (55.3–67.9 mol%) reported from some species of the genus Pseudomonas (Liu et al , 2013; Lin et al , 2013a, b; Clark et al , 2006; Feng et al , 2012; Hameed et al , 2014). The polar lipid profile of strain CC-MHH0089 T was similar to those of P. azotifigens JCM 12708 T and P. balearica BCRC 17184 T with respect to the presence of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol, which is in agreement with data published previously for species of the genus Pseudomonas (Pascual et al , 2012; Lin et al , 2013a, b; Liu et al , 2013; Hameed et al , 2014). In addition, moderate amounts of phosphatidylcholine and an unidentified phospholipid were also detected, though there were some quantitative differences ().…”

Phylogenomic and comparative genomic analyses of species of the family Pseudomonadaceae: Proposals for the genera Halopseudomonas gen. nov. and Atopomonas gen. nov., merger of the genus Oblitimonas with the genus Thiopseudomonas, and transfer of some misclassified species of the genus Pseudomonas into other genera

“…Hence, we are proposing to integrate Oblitimonas with Thiopseudomonas and transfer P. caeni into this genus as a new name combination. P. hussainii is another deep-branching species [58], which lies outside of the main cluster of species of the genus Pseudomonas. This species is assigned into Pseudomonas_L clade by the GTDB webserver [29].…”

“…The description of this species is as given by Hwang et al [66] for Pseudomonas pelagia. Cells are Gram-stain negative, aerobic, motile by means of a monopolar flagellum, non-spore-forming, chemoheterotrophic and mesophilic [58]. Growth occurs at 15-40 °C (optimum, 30-37 °C), at pH 6.0-8.0 (optimum, pH 7.0) and on R2A agar supplemented with 0-5 % NaCl (optimum, 1%).…”

Phylogenomic Analyses of the Genus Pseudomonas Lead to the Rearrangement of Several Species and the Definition of New Genera

“…The description is as given for Pseudomonas hussainii [ 55 ] with the following modification. The genomic G + C is 58.8% and its genomic size is approximately 3.68 Mbp.…”