Pseudomonas aeruginosa-producing Metallo-β-lactamases (VIM, IMP, SME, and AIM) in the Clinical Isolates of Intensive Care Units, a University Hospital in Isfahan, Iran

Abstract

Background:Pseudomonas aeruginosa is a severe challenge for antimicrobial therapy, due to the chromosomal mutations or exhibition of intrinsic resistance to various antimicrobial agents such as most β-lactams. We undertook this study to evaluate the existence of SME, IMP, AIM, and VIM metallo-β-lactamases (MBL) encoding genes among P. aeruginosa strains isolated from Intensive Care Unit (ICU) patients in Al-Zahra Hospital in Isfahan, Iran.Materials and Methods:In a retrospective cross-sectional study that was conducted between March 2012 and April 2013, a total of 48 strains of P. aeruginosa were collected from clinical specimens of bedridden patients in ICU wards. Susceptibility test was performed by disc diffusion method. All of the meropenem-resistant strains were subjected to modified Hodge test for detection of carbapenemases. Multiplex polymerase chain reaction was performed for detection of blaVIM, blaIMP, blaAIM, and blaSME genes.Results:In disk diffusion method, imipenem and meropenem showed the most and colistin the least resistant antimicrobial agents against P. aeruginosa strains. Of the 48 isolates, 36 (75%) were multidrug resistant (MDR). Amplification of β-lactamase genes showed the presence of blaVIM genes in 7 (%14.6) strains and blaIMP genes in 15 (31.3%) strains. All of the isolates were negative for blaSME and blaAIM genes. We could not find any statistically significant difference among the presence of this gene and MDR positive, age, or source of the specimen.Conclusion:As patients with infections caused by MBL-producing bacteria are at an intensified risk of treatment failure, fast determination of these organisms is necessary. Our findings may provide useful insights in replace of the appropriate antibiotics and may also prevent MBLs mediated resistance problem.

Molecular Detection of Serine Carbapenemase Genes in Carbapenem-Resistant Isolates of Pseudomonas aeruginosa Recovered from Patients in Al-Diwaniyah Province, Iraq

“…The enzyme included three types: SME-1, SME-2, and SME-3, identified in different parts of the United States (Queenan and Bush, 2007;Walther-Rasmussen and H‫ר‬iby, 2007). While these enzymes were not previously determined in our country, our results were contrasted with the results of local and global studies to investigate these enzymes in P. aeruginosa isolates (Al-Shara, 2013; Khorvash et al, 2017).…”

Understanding the Epidemiology of Multi-Drug Resistant Gram-Negative Bacilli in the Middle East Using a One Health Approach

“…in clinical settings (Azizi et al, 2015; Zanganeh and Eftekhar, 2015; Shoja et al, 2016, 2017; Mohajeri et al, 2017; Sarikhani et al, 2017; Zafari et al, 2017; Rezaei et al, 2018; Shirmohammadlou et al, 2018). Other carbapenem resistance genes included OXA-24, OXA-58, IMP, VIM, KPC, GIM, SIM, and SPM (Peymani et al, 2011; Azimi et al, 2015; Bagheri Josheghani et al, 2015; Aghamiri et al, 2016; Maspi et al, 2016; Moghadam et al, 2016; Khorvash et al, 2017; Armin et al, 2018; Soltani et al, 2018). Isolated strains of carbapenem resistant A. baumannii are genetically diverse with the predominance of International clone I and II (Peymani et al, 2011; Savari et al, 2017; Mahdian et al, 2015; Sarhaddi et al, 2017).…”

Microbiological and Molecular Genetic Aspects of Antibiotic Resistance of <i>Pseudomonas aeruginosa</i> and <i>Acinetobacter baumannii</i>

“…Среди выделенных штаммов 100% были резистентны к цефалоспоринам 2 поколения (цефоперазон) и фосфомицинам, высокий уровень резистентности был выявлен к неустойчивым к β-лактамазе пенициллинам и β-лактамным пенициллинам, цефалоспоринам 4 поколения (93,9 ± 2,4%). Эффективным [5,6]. Частота встречаемости была следующей: у P. aeruginosa ген blaIMP определялся в 19% случаев, ген blaVIMв 45%.…”

“…Гены, кодирующие МБЛ, входят в состав плазмид, что способствует их быстрому внутри-и межвидовому распространению. Оно часто носит эпидемический характер, при этом доминируют определенные штаммы или ферменты в масштабах как отдельных центров, так и обширных географических зон [4][5][6]. Популяции микроорганизмов, складывающиеся в условиях стационара, всегда состоят из штаммов с высокой вирулентностью и антибиотикоустойчивостью.…”