Pseudomonas aeruginosa KUCd1, a possible candidate for cadmium bioremediation

Sinha, Sangram; Mukherjee, Samir Kumar

doi:10.1590/s1517-83822009000300030

Cited by 61 publications

(31 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bacteria employ various mechanisms to survive in stress environments, like detoxification, efflux mechanisms, adsorption, and compartmentalization . Cd uptake by Pseudomonas species has also been studied earlier . Our findings with dead cells of P. putida mt2 are also in agreement with those of Kurek et al .…”

Section: Discussionsupporting

confidence: 92%

Antioxidative enzyme profiling and biosorption ability of Cupriavidus metallidurans CH34 and Pseudomonas putida mt2 under cadmium stress

Shamim

Rehman

2013

J. Basic Microbiol.

View full text Add to dashboard Cite

Cupriavidus metallidurans CH34 and Pseudomonas putida mt2 were used as cadmium (Cd)-resistant and -sensitive bacteria, respectively, to study their biosorption ability and their antioxidative enzymes. The minimal inhibitory concentration of C. metallidurans CH34 for Cd was found to be 30 mM, and for P. putida mt2 it was 1.25 mM. The tube dilution method revealed the heavy-metal resistance pattern of C. metallidurans CH34 as Ni(2+) (10 mM)>Zn(2+) (4 mM)>Cu(2+) (2 mM)>Hg(2+) (1 mM)>Cr(2+) (1 mM)>Pb(2+) (0 mM), whereas P. putida mt2 was only resistant to Zn(2+) (1 mM). Under Cd stress, the induction of GSH was higher in C. metallidurans CH34 (0.359 ± 0.010 mM g(-1) FW) than in P. putida mt2 (0.286 ± 0.005 mM g(-1) FW). Glutathione reductase was more highly expressed in the mt2 strain, in contrast to non-protein thiols and peroxidase. Unlike dead bacterial cells, live cells of both bacteria showed significant Cd biosorption, i.e. more than 80% at 48 h. C. metallidurans CH34 used only catalase, whereas P. putida mt2 used superoxide dismutase and ascorbate peroxidase to combat Cd stress. This study investigated the Cd biosorption ability and enzymes involved in the Cd detoxification mechanisms of C. metallidurans CH34 and P. putida mt2.

show abstract

Section: Discussionsupporting

confidence: 92%

Antioxidative enzyme profiling and biosorption ability of Cupriavidus metallidurans CH34 and Pseudomonas putida mt2 under cadmium stress

Shamim

Rehman

2013

J. Basic Microbiol.

View full text Add to dashboard Cite

show abstract

“…Furthermore, many investigators have reported the bioaccumulation of cadmium onto natural microbial populations like bacteria and algae as the new bioremediation technology [9,10]. The microbes use various survival strategies to combat the cadmium stress that include metal ions sequestration, active efflux of metals; some use enzymatic detoxification and cadmium accumulation [11,12].…”

Section: Introductionmentioning

confidence: 99%

Isolation, Identification, and Characterization of Cadmium Resistant Pseudomonas sp. M3 from Industrial Wastewater

Rafatullah

Ismail

Lalung

2014

Journal of Waste Management

View full text Add to dashboard Cite

The present study deals with the isolation, identification, and characterization of the cadmium resistant bacteria from wastewater collected from industrial area of Penang, Malaysia. The isolate was selected based on high level of the cadmium and antibiotic resistances. On the basis of morphological, biochemical characteristics, 16S rDNA gene sequencing and phylogeny analysis revealed that the strain RZCd1 was authentically identified asPseudomonassp. M3. The industrial isolate showed more than 70% of the cadmium removal in log phase. The cadmium removal capacity of strain RZCd1 was affected by temperature and pH. At pH 7.0 and 35°C, strain RZCd1 showed maximum cadmium removal capacity. The minimal inhibitory concentration of strain RZCd1 against the cadmium was 550 µg/mL. The resistance against the cadmium was associated with resistance to multiple antibiotics: amoxicillin, penicillin, cephalexin, erythromycin, and streptomycin. The strain RZCd1 also gave thick bands of proteins in front of 25 kDa in cadmium stress condition after 3 h of incubation. So the identified cadmium resistant bacteria may be useful for the bioremediation of cadmium contaminated industrial wastewater.

show abstract

“…The approach suffers from the fact that in the presence of cadmium, sulfate reduction rate gets reduced (Pagnanelli et al 2010). Bioaccumulation of cadmium by a cadmium-resistant Pseudomonas aeruginosa strain KUCd1 was also reported by Sinha and Mukherjee (2009) at pH 8.5, under strict aerobic conditions as well as continuous supply of nutrients. On the other hand, precipitation of cadmium as cadmium sulfide via hydrogen sulfide generation under completely anaerobic conditions in Pseudomonas aeruginosa, Salmonella typhimurium, and Treponema denticola has been reported by several authors (Wang et al 1997(Wang et al , 2000(Wang et al , 2001White and Gadd 1996;Bang et al 2000).…”

Section: Bioprecipitation Of Metal Ions From Single-ion Solutionsmentioning

confidence: 63%

Application of alkaline phosphatase for heavy metals precipitation using ascorbic acid 2-phosphate as an effective natural substrate

Chaudhuri

Venu-Babu

Dalal

et al. 2015

Int. J. Environ. Sci. Technol.

View full text Add to dashboard Cite

show abstract

Pseudomonas aeruginosa KUCd1, a possible candidate for cadmium bioremediation

Cited by 61 publications

References 13 publications

Antioxidative enzyme profiling and biosorption ability of Cupriavidus metallidurans CH34 and Pseudomonas putida mt2 under cadmium stress

Antioxidative enzyme profiling and biosorption ability of Cupriavidus metallidurans CH34 and Pseudomonas putida mt2 under cadmium stress

Isolation, Identification, and Characterization of Cadmium Resistant Pseudomonas sp. M3 from Industrial Wastewater

Application of alkaline phosphatase for heavy metals precipitation using ascorbic acid 2-phosphate as an effective natural substrate

Contact Info

Product

Resources

About