Application of alkaline phosphatase for heavy metals precipitation using ascorbic acid 2-phosphate as an effective natural substrate

Chaudhuri, Gouri; Venu-Babu, P.; Dalal, D.; Thilagaraj, Wilson Richard

doi:10.1007/s13762-014-0749-y

Cited by 8 publications

(3 citation statements)

References 36 publications

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“…The average precipitation pattern of metal ions from single-ion solutions by BAP and CIAP occurred in the order of Cd effluents with the percentage of 94.6 and 66, respectively. Since ascorbic acid 2-phosphate is a natural and biodegradable substrate, this study offers an eco-friendly approach for a sustainable environment [25].…”

Section: +mentioning

confidence: 99%

“…Therefore, no scavenging of P i is expected and the amount of inorganic phosphate liberated for metal-phosphate formation should be constant. In addition, the variation in the precipitation with respect to different ions might be because of the difference in kinetics of metal-phosphate formation and also due to the radical effect of metal ions present in the enzyme [25].…”

Section: Average Precipitation Of Heavy Metals From Single-ion Solutimentioning

confidence: 99%

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Recent Trends in Phosphatase-Mediated Bioremediation

Chaudhuri¹,

Selvaraj²,

Babu³

et al. 2017

Phosphoric Acid Industry - Problems and Solutions

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Industrial effluents from tanneries and electroplating industries from small-and large-scale sector industrial plants contain substantial amount of toxic heavy metal, which pollutes rivers and lakes, land, air and sea leading to imbalance of ecosystem and certain health issues to humans, animals as well as plants. The worldwide environmental regulations stipulate the reduction of heavy metals in the effluents to permissible levels before discharging into water bodies. Enzyme-mediated precipitation of heavy metals affords a novel eco-friendly method for remediation of toxic heavy metals from various industrial effluents like tannery, electroplating and dye industries. This chapter has paid attention to bacterial alkaline phosphatase (BAP) from Escherichia coli C90 and calf-intestinal alkaline phosphatase (CIAP), which catalyses phospho mono-and diesters and produces inorganic phosphate (Pi). The Pi thus generated precipitates the heavy metals as metal-phosphate complexes. The kinetic behaviour of both the enzymes with para-nitrophenyl phosphate, ascorbic acid 2-phosphate and α-naphthyl phosphate was investigated at various pH regimes from 8 to 11. The chapter also explains in detail the descriptive information on the capability of BAP-and CIAP-mediated precipitation of heavy metals, which is desirable and convenient method for the toxic heavy metals such as chromium, cadmium, nickel and cobalt.

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Section: +mentioning

confidence: 99%

Section: Average Precipitation Of Heavy Metals From Single-ion Solutimentioning

confidence: 99%

Recent Trends in Phosphatase-Mediated Bioremediation

Chaudhuri¹,

Selvaraj²,

Babu³

et al. 2017

Phosphoric Acid Industry - Problems and Solutions

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“…Currently, the biotechnological potential of ALP makes it gain enormous attention in white biotechnology 2 . Phosphatases are one of the most crucial enzymes for an organism’s survival and play a vital role in phosphate transportation and metabolism under phosphate-deficient conditions 3 .…”

Section: Introductionmentioning

confidence: 99%

Biovalorization of raw agro-industrial waste through a bioprocess development platform for boosting alkaline phosphatase production by Lysinibacillus sp. strain APSO

Abdelgalil

Soliman

Abo-Zaid

et al. 2021

Sci Rep

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This study highlighted the exploitation of mathematical models for optimizing the growth conditions that give the highest phosphatase productivity from a newfound Lysinibacillus sp. strain APSO isolated from a slime sample. Mathematical models facilitate data interpretation and provide a strategy to solve fermentation problems. Alkaline phosphatase (ALP) throughput was enhanced by 16.5-fold compared to basal medium based on a sequential optimization strategy that depended on two-level Plackett–Burman design and central composite design. The additional improvement for volumetric productivity and specific production yield was followed in a 7 L bench-top bioreactor to evaluate microbial growth kinetics under controlled and uncontrolled pH conditions. The pH-controlled batch cultivation condition neither supported cell growth nor enhanced ALP productivity. In contrast, the uncontrolled pH batch cultivation condition provided the highest ALP output (7119.4 U L−1) and specific growth rate (µ = 0.188 h−1) at 15 h from incubation time, which was augmented > 20.75-fold compared to the basal medium. To the authors’ knowledge, this study is the second report that deals with how to reduce the production cost of the ALP production process via utilization of agro-industrial waste, such as molasses and food waste (eggshell), as a nutrimental source for the improvement of the newfound Lysinibacillus sp. strain APSO ALP throughput.

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A new approach using polyvinylidene fluoride immobilised calf-intestinal alkaline phosphatase for uranium bioprecipitation

Venu-Babu

Chaudhuri

Thilagaraj

2017

Int. J. Environ. Sci. Technol.

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Uranium is a predominant radionuclide arising out of operations of mining, milling and fuel fabrication for nuclear power plants. While chemical and biological methods using several micro-organisms have been employed for the remediation of uranium from such effluents, use of immobilised enzymes for the purpose has not been reported. The current investigation deals with a purified calf-intestinal alkaline phosphatase which is capable of driving uranium precipitation in the presence of a phosphate-containing substrate. The enzyme, both in free form and in an immobilised form on polyvinylidene fluoride membrane, was investigated for the precipitation of uranium using para-nitrophenyl phosphate as substrate. The results indicated that while the enzyme in free form could precipitate most of the uranium within 6 h, the immobilised enzyme could achieve the same in 24 h. There was a spurt in the precipitation of uranium by the immobilised enzyme during the initial period of 3 h followed by a linear increase for the remaining period. The immobilised enzyme was found to be stable and catalytically active for 3 years in precipitating uranium. The results obtained offer polyvinylidene fluoride immobilised alkaline phosphatase from calf intestine as a new tool for the remediation of uranium from effluents. Further, evidence has been also shown that no requirement for any cellular architecture/ scaffolding exists for uranium mineralisation to occur.

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Application of alkaline phosphatase for heavy metals precipitation using ascorbic acid 2-phosphate as an effective natural substrate

Abstract: The utility of alkaline phosphatase in the precipitation of heavy metals such as Ni

Cited by 8 publications

References 36 publications

Recent Trends in Phosphatase-Mediated Bioremediation

Recent Trends in Phosphatase-Mediated Bioremediation

Biovalorization of raw agro-industrial waste through a bioprocess development platform for boosting alkaline phosphatase production by Lysinibacillus sp. strain APSO

A new approach using polyvinylidene fluoride immobilised calf-intestinal alkaline phosphatase for uranium bioprecipitation

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