Recent Trends in Phosphatase-Mediated Bioremediation

Abstract

Industrial effluents from tanneries and electroplating industries from small-and large-scale sector industrial plants contain substantial amount of toxic heavy metal, which pollutes rivers and lakes, land, air and sea leading to imbalance of ecosystem and certain health issues to humans, animals as well as plants. The worldwide environmental regulations stipulate the reduction of heavy metals in the effluents to permissible levels before discharging into water bodies. Enzyme-mediated precipitation of heavy metals affords a novel eco-friendly method for remediation of toxic heavy metals from various industrial effluents like tannery, electroplating and dye industries. This chapter has paid attention to bacterial alkaline phosphatase (BAP) from Escherichia coli C90 and calf-intestinal alkaline phosphatase (CIAP), which catalyses phospho mono-and diesters and produces inorganic phosphate (Pi). The Pi thus generated precipitates the heavy metals as metal-phosphate complexes. The kinetic behaviour of both the enzymes with para-nitrophenyl phosphate, ascorbic acid 2-phosphate and α-naphthyl phosphate was investigated at various pH regimes from 8 to 11. The chapter also explains in detail the descriptive information on the capability of BAP-and CIAP-mediated precipitation of heavy metals, which is desirable and convenient method for the toxic heavy metals such as chromium, cadmium, nickel and cobalt.

The Interaction Effect of Laser Irradiation and 6-Benzylaminopurine Improves the Chemical Composition and Biological Activities of Linseed (Linum usitatissimum) Sprouts

“…According to Mangena [ 32 ], the application of BAP increase the growth parameters of soybean sprouts. This report, together with that of Chaudhuri [ 33 ], emphasized the role of BAP in increasing the plant biomass. Interestingly, the combined physical and chemical priming seemed to enhance the growth parameters in linseed sprouts and reach their highest level, as compared to control and both laser light and BAP treatment separately.…”

Dynamic consolidated bioprocessing for innovative lab-scale production of bacterial alkaline phosphatase from Bacillus paralicheniformis strain APSO

“…These results are consistent with those obtained by Behera and others 16 , who reported that the maximum yield of ALP from Alcaligenes faecalis was achieved at 45 °C. The pH of the fermentation broth influences the metabolic activity of microorganisms and thus plays a significant role in the optimization of the fermentation processes; the catalytic activity of ALP has been reported to be regulated mainly by Tris buffers 18 . The obtained results demonstrated that the maximum ALP productivity was recorded at pH values of 9 and 10 (31.7 and 27.19 U L -1 , respectively), with a notable falling off in productivity of ALP for slightly acidic (pH 6.0), neutral (pH 7.0) and slightly alkaline (pH 8.0) conditions to activities of 6.64, 11.46, and 15.76 U L -1 , respectively.…”

Bioprocess development as a sustainable platform for eco-friendly alkaline phosphatase production: an approach towards crab shells waste management

“…This will be accomplished via the use of cost-effective and sustainable production strategies. Thus, bacterial ALP production in a cost-effective manner, by utilizing bioresources such as biowaste, as well as the effects of the whole bioprocessing process on the environment are two components of sustainable bioprocessing management [ 9 ].…”