Pseudomonas aeruginosa in public water supply

Abstract

Water is indispensable for life and its quality is directly related to public health. The objective of this study was to investigate the presence of Pseudomonas aeruginosa in public water supply in municipalities in São Paulo State, Brazil. Analyses were carried out at the Adolfo Lutz Institute between February and December 2016, and included physicochemical (chlorine, pH, color, turbidity and nitrate), and microbiological parameters (total coliforms and Escherichia coli), as well as P. aeruginosa, with susceptibility tests to antimicrobial agents and biofilm production capacity by the strains isolated. In total, 251 water samples were evaluated and 19 (7.6%) presented P. aeruginosa. No significant differences were observed between the physicochemical parameters found in the positive and negative samples for this bacterium, but the samples containing total coliforms were also those with the highest positivity for P. aeruginosa. All samples with P. aeruginosa reported chlorine concentrations between 0.2 and 2.0 mg/L, as required by Brazilian legislation, demonstrating this bacterium's resistance to conventional water treatment processes. Although not resistant to the antimicrobials tested, most strains isolated were classified as strong biofilm producers, emphasizing the need for further studies involving water supply quality.

Virulence properties and antimicrobial resistance of Pseudomonas aeruginosa isolated from cave waters at Roraima Tepui, Guayana Highlands

“…2017; Anversa et al . 2019). Environmental or non‐pathogenic forms of the bacterium may serve as a storehouse of genetic determinants which, if transferred to other bacterial strains, may confer novel virulence capabilities (Moradali et al 2017).…”

“…Recent studies suggest that water systems are increasingly becoming a preferred interface in the epidemiology of P. aeruginosa (Flores et al 2014;Vaz-Moreira et al 2014;Pappa et al 2016;Schiavano et al 2017;Anversa et al 2019). Environmental or non-pathogenic forms of the bacterium may serve as a storehouse of genetic determinants which, if transferred to other bacterial strains, may confer novel virulence capabilities (Moradali et al 2017).…”

Subinhibitory Concentrations of Biogenic Silver Nanoparticles Affect Motility and Biofilm Formation in Pseudomonas aeruginosa

“…AgNPs were shown to have antimicrobial properties against several pathogenic microorganisms ( Scandorieiro et al., 2016 ; Figueiredo et al., 2019 ), including Pseudomonas aeruginosa ( Liao et al., 2019 ; D’Lima et al., 2020 ). This bacterium can be found in various environments, such as hospitals, natural resources, and water supply systems ( Dobias and Bernier-Latmani, 2013 ; Anversa et al., 2019 ; Esmaeili et al., 2019 ). P. aeruginosa may express different virulence factors and is closely related to healthcare-related infections and diseases in immunocompromised, cystic fibrosis, and severely burned patients.…”

Pesquisa fenotípica dos fatores de virulência em Pseudomonas aeruginosa isolados de água de abastecimento público

“…Pseudomonas aeruginosa é uma bactéria Gram-negativa amplamente distribuída no ambiente, presente no solo, na água e na matéria orgânica em decomposição. É frequentemente responsável por infecções no trato urinário e respiratório, podendo evoluir para sepse, principalmente em portadores de fibrose cística e pacientes imunocomprometidos ou com extensas queimaduras na pele [1,2]. É o agente mais comum em infecções relacionadas à assistência à saúde (IRAS), associada com significativa taxa de morbi-mortalidade devido à capacidade do microrganismo de desenvolver rapidamente resistência a antibióticos, adaptar-se facilmente às mudanças no ambiente, e expressar uma variedade de fatores de virulência, como motilidade, formação de biofilme, síntese de protease alcalina, de ramnolipídeos e de DNases [3].…”

“…A DNase é outro fator de virulência que auxilia a bactéria na fuga de neutrófilos ligados a captura e eliminação de invasores [12]. Desta forma, bactérias como P. aeruginosa produtoras de diversos fatores de virulência podem estar presentes no ambiente, inclusive em sistemas de abastecimento de água [2].…”