Pseudomonas aeruginosa-derived exosomes ameliorates allergic reactions via inducing the Treg response in asthma

Ding, Fengxia; Liu, Bo; Zou, Wenjing; Li, Qubei; Tian, Daiyin; Fu, Zhou

doi:10.1038/s41390-018-0020-1

Cited by 13 publications

(12 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, the inhibitory effect of exosomes isolated from Pseudomonas aeruginosa on the development of AHR along with reduced inflammation in the perivascular and peribronchial spaces in allergic asthma model has also been reported. This inhibitory effect was attributed to the increased Treg and attenuated Th2 responses on treatment with P• aeruginosa derived exosomes [249].…”

Section: Copd/asthmamentioning

confidence: 99%

Extracellular vesicles: novel communicators in lung diseases

et al. 2020

View full text Add to dashboard Cite

The lung is the organ with the highest vascular density in the human body. It is therefore perceivable that the endothelium of the lung contributes significantly to the circulation of extracellular vesicles (EVs), which include exosomes, microvesicles, and apoptotic bodies. In addition to the endothelium, EVs may arise from alveolar macrophages, fibroblasts and epithelial cells. Because EVs harbor cargo molecules, such as miRNA, mRNA, and proteins, these intercellular communicators provide important insight into the health and disease condition of donor cells and may serve as useful biomarkers of lung disease processes. This comprehensive review focuses on what is currently known about the role of EVs as markers and mediators of lung pathologies including COPD, pulmonary hypertension, asthma, lung cancer and ALI/ARDS. We also explore the role EVs can potentially serve as therapeutics for these lung diseases when released from healthy progenitor cells, such as mesenchymal stem cells. Extracellular vesicles Cell-to-cell communication is essential for nearly all physiologic and metabolic processes. This intercellular conveyance is achieved through receptor ligands, signaling molecules, hormones, and extracellular vesicles (EVs). Historically, secretion of the cell in the form of EVs was considered as unimportant waste material, cellular "garbage bags," or dust particles [1-5]. However, in recent years, this so-called "waste" is now known to be of profound importance in various biological systems, creating a boon in their exploration across the scientific community. Lipid bilayer membrane-enclosed vesicles are secreted by both prokaryotic and eukaryotic cells [4-9]. Although, the term "extracellular vesicle" is sometimes used in reference to exosomes, it is actually a very broad term that encompasses all different types of vesicles secreted outside the cells [10]. Regardless, the function of all these vesicles appears to be all the same: communication between the cells within an organism or between species [11, 12]. In addition, it is not necessary that all vesicles secreted from cells are functional or have any role in some kind of biological process. Sometimes, they just act as "dustcart" to remove the waste from cells [13]. Of note, there have been discrepancies in the classification of these vesicles in the literature. Some studies divide EVs into two major categories: I) exosomes, defined as vesicles released by exocytosis of the multivesicular bodies; and II) ectosomes, defined as the vesicles which are assembled and released by the plasma membrane [14]. However, most recent studies categorize EVs as either exosome, microvesicles, microparticles, or apoptotic bodies based on vesicle size and how they are formed [15-21] (Fig. 1). Exosomes Exosomes are small EVs with sizes ranging between 30 and 150 nm in diameter that originate from the internal vesicles of multivesicular bodies (MVB) of nearly all cell types. Exosomes originating from different cell types have different composition; however, there are certain

show abstract

Section: Copd/asthmamentioning

confidence: 99%

Extracellular vesicles: novel communicators in lung diseases

et al. 2020

View full text Add to dashboard Cite

show abstract

“…For example, Lactobacillus casei rhamnosus Lcr35, a species of Lactobacillus (1st in the prediction list), was found to be able to attenuate airway inflammation and hyperreactivity in a mouse model of asthma through oral treatment before sensitization (Yu et al, 2010 ). Besides, Ding et al ( 2018 ) discovered that exosomes derived by Pseudomonas (2nd in the prediction list) aeruginosa could induce protection against allergic sensitization in asthma mice. Another example is that there is a distinct alteration of the sputum microbiota with a greater prominence of Firmicutes (4th in the prediction list) in severe asthma (Zhang et al, 2016 ).…”

Section: Resultsmentioning

confidence: 99%

Human Microbe-Disease Association Prediction With Graph Regularized Non-Negative Matrix Factorization

Peng

2018

Front. Microbiol.

View full text Add to dashboard Cite

A microbe is a microscopic organism which may exists in its single-celled form or in a colony of cells. In recent years, accumulating researchers have been engaged in the field of uncovering microbe-disease associations since microbes are found to be closely related to the prevention, diagnosis, and treatment of many complex human diseases. As an effective supplement to the traditional experiment, more and more computational models based on various algorithms have been proposed for microbe-disease association prediction to improve efficiency and cost savings. In this work, we developed a novel predictive model of Graph Regularized Non-negative Matrix Factorization for Human Microbe-Disease Association prediction (GRNMFHMDA). Initially, microbe similarity and disease similarity were constructed on the basis of the symptom-based disease similarity and Gaussian interaction profile kernel similarity for microbes and diseases. Subsequently, it is worth noting that we utilized a preprocessing step in which unknown microbe-disease pairs were assigned associated likelihood scores to avoid the possible negative impact on the prediction performance. Finally, we implemented a graph regularized non-negative matrix factorization framework to identify potential associations for all diseases simultaneously. To assess the performance of our model, cross validations including global leave-one-out cross validation (LOOCV) and local LOOCV were implemented. The AUCs of 0.8715 (global LOOCV) and 0.7898 (local LOOCV) proved the reliable performance of our computational model. In addition, we carried out two types of case studies on three different human diseases to further analyze the prediction performance of GRNMFHMDA, in which most of the top 10 predicted disease-related microbes were verified by database HMDAD or experimental literatures.

show abstract

“…Subsequent studies conducted in a larger group of individuals suggested that the presence of IgG antibodies against indoor dust EVs in serum is a major risk factor for the development of asthma, COPD, and lung cancer [ 129 ]. On the other hand, intranasal pretreatment with exosomes isolated from Pseudomonas aeruginosa was able to ameliorate AHR, allergic inflammation, and atopic sensitization levels in mice subjected to OVA- and HDM-based models of AAI [ 130 ]. Furthermore, these protective effects were accompanied by an enhancement of Treg responses and a simultaneous decrease in type-2-driven immune mechanisms [ 130 ].…”

Section: Extracellular Vesicles and Asthma: Microorganisms And Other External Influencesmentioning

confidence: 99%

“…On the other hand, intranasal pretreatment with exosomes isolated from Pseudomonas aeruginosa was able to ameliorate AHR, allergic inflammation, and atopic sensitization levels in mice subjected to OVA- and HDM-based models of AAI [ 130 ]. Furthermore, these protective effects were accompanied by an enhancement of Treg responses and a simultaneous decrease in type-2-driven immune mechanisms [ 130 ].…”

Section: Extracellular Vesicles and Asthma: Microorganisms And Other External Influencesmentioning

confidence: 99%

Extracellular Vesicles and Asthma—More Than Just a Co-Existence

Alhamwe

Potaczek

Miethe

et al. 2021

IJMS

View full text Add to dashboard Cite

Extracellular vesicles (EVs) are membranous structures, which are secreted by almost every cell type analyzed so far. In addition to their importance for cell-cell communication under physiological conditions, EVs are also released during pathogenesis and mechanistically contribute to this process. Here we summarize their functional relevance in asthma, one of the most common chronic non-communicable diseases. Asthma is a complex persistent inflammatory disorder of the airways characterized by reversible airflow obstruction and, from a long-term perspective, airway remodeling. Overall, mechanistic studies summarized here indicate the importance of different subtypes of EVs and their variable cargoes in the functioning of the pathways underlying asthma, and show some interesting potential for the development of future therapeutic interventions. Association studies in turn demonstrate a good diagnostic potential of EVs in asthma.

show abstract

Pseudomonas aeruginosa-derived exosomes ameliorates allergic reactions via inducing the Treg response in asthma

Abstract: In conclusion, these observations demonstrated that P. aeruginosa-derived exosomes could induce protection against allergic sensitization in asthma mice, and our study provided a new insight to prevent allergic diseases.

Cited by 13 publications

References 34 publications

Extracellular vesicles: novel communicators in lung diseases

Extracellular vesicles: novel communicators in lung diseases

Human Microbe-Disease Association Prediction With Graph Regularized Non-Negative Matrix Factorization

Extracellular Vesicles and Asthma—More Than Just a Co-Existence

Contact Info

Product

Resources

About