Pseudomonas aeruginosa bacteremia in patients with liver cirrhosis: a comparison with bacteremia caused by Enterobacteriaceae

Bang, Ji Hwan; Jung, Younghee; Cheon, Shinhye; Kim, Chung Jong; Song, Kyung Ho; Choe, Pyeong Gyun; Park, Wan Beom; Kim, Eu Suk; Park, Sang Won; Kim, Hong Bin; Oh, Myoung Don; Lee, Hyo-Suk; Kim, Nam Joong

doi:10.1186/1471-2334-13-332

Cited by 9 publications

(5 citation statements)

References 25 publications

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“…8 However, in many cases, especially with the emergence of multidrug-resistant strains, 53,54 these methods are not efficient, and therefore, additional means of disinfecting wounds are clearly needed. Furthermore, P. aeruginosa and S. epidermidis can cause deep infections in many tissue sites, including joints, 10,55 lung, heart, 56 liver, 57 and implants. 10 To address these problems, we recently proposed to use non-thermal, high-voltage PEF technology, previously found to be effective for wounds and surgical mesh disinfection.…”

Section: Clinical Relevancementioning

confidence: 99%

Selective Inactivation ofPseudomonas aeruginosaandStaphylococcus epidermidiswith Pulsed Electric Fields and Antibiotics

Rubin

Usta

Schloss

et al. 2019

Advances in Wound Care

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Increasing numbers of multidrug-resistant bacteria make many antibiotics ineffective; therefore, new approaches to combat microbial infections are needed. In addition, antibiotics are not selective-they kill pathogenic organisms as well as organisms that could positively contribute to wound healing (bio flora). Approach: Here we report on selective inactivation of Pseudomonas aeruginosa and Staphylococcus epidermidis, potential pathogens involved in wound infections with pulsed electric fields (PEFs) and antibiotics (mix of penicillin, streptomycin, and nystatin). Results: Using a Taguchi experimental design in vitro, we found that, under similar electric field strengths, the pulse duration is the most important parameter for P. aeruginosa inactivation, followed by the number of pulses and pulse frequency. P. aeruginosa, a potential severe pathogen, is more sensitive than the less pathogenic S. epidermidis to PEF (alone or in combination with antibiotics). Applying 200 pulses with a duration of 60 ls at 2.8 Hz, the minimum electric fields of 308.8-28.3 and 378.4-12.9 V/mm were required to inactive P. aeruginosa and S. epidermidis, respectively. Addition of antibiotics reduced the threshold for minimum electric fields required to inactivate the bacteria. Innovation: This study provides essential information, such as critical electric field parameters for bacteria inactivation, required for developing in vivo treatment and clinical protocols for using PEF for wound healing. Conclusion: A combination of PEFs with antibiotics reduces the electric field threshold required for bacteria disinfection. Such an approach simplifies devices required to disinfect large areas of infected wounds.

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Section: Clinical Relevancementioning

confidence: 99%

Selective Inactivation ofPseudomonas aeruginosaandStaphylococcus epidermidiswith Pulsed Electric Fields and Antibiotics

Rubin

Usta

Schloss

et al. 2019

Advances in Wound Care

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“…Overall, these results further illustrate the potential role of biotransformation with CYP enzymes in the production of natural product derivatives. Given the role of P. aeruginosa as a human pathogen, which can cause infections in the liver, small intestine, and urinary tract, − and the importance of alkylquinolones in modulating pathogenicity, the oxidative degradation of these compounds by CYP4F11 could also have implications for human health.…”

Section: Resultsmentioning

confidence: 99%

Expected and Unexpected Products from the Biochemical Oxidation of Bacterial Alkylquinolones with CYP4F11

Shi,

Li,

Wolf

et al. 2023

J. Nat. Prod.

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2-Alkylquinolones are a class of microbial natural products primarily produced in the Pseudomonas and Burkholderia genera that play a key role in modulating quorum sensing. Bacterial alkylquinolones were synthesized and then subjected to oxidative biotransformation using human cytochrome P450 enzyme CYP4F11, heterologously expressed in the fission yeast Schizosaccharomyces pombe. This yielded a range of hydroxylated and carboxylic acid derivatives which had undergone ω-oxidation of the 2-alkyl chain, the structures of which were determined by analysis of NMR and MS data. Oxidation efficiency depended on chain length, with a chain length of eight or nine carbon atoms proving optimal for high yields. Homology modeling suggested that Glu233 was relevant for binding, due to the formation of a hydrogen bond from the quinolone nitrogen to Glu233, and in this position only the longer alkyl chains could come close enough to the heme moiety for effective oxidation. In addition to the direct oxidation products, a number of esters were also isolated, which was attributed to the action of endogenous yeast enzymes on the newly formed ω-hydroxy-alkylquinolones. ω-Oxidation of the alkyl chain significantly reduced the antimicrobial and antibiofilm activity of the quinolones.

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“…Sera from P. aeruginosa -infected patients displayed elevated levels of bisecting GlcNAcylation and core fucosylation, and a concomitant decrease in sialylation. Being an opportunistic gram-negative pathogen, P. aeruginosa is closely associated with cystic fibrosis [ 63 , 64 ], affecting lung and liver [ 65 , 66 ], and has been reported to alter the host glycome by modulation of the complex- and paucimannosidic-type N -glycans (discussed below) in peripheral tissues and in blood [ 54 , 67 ]. Albeit less studied, P. aeruginosa -based bloodstream infections have been reported to lead to an elevation of circulating immunoglobulins [ 68 ] and acute-phase glycoproteins such as α-1-antitrypsin, fibrinogen, and haptoglobin [ 69 ].…”

Section: Discussionmentioning

confidence: 99%

Serum N-Glycomics Stratifies Bacteremic Patients Infected with Different Pathogens

Chatterjee

Kawahara

Tjondro

et al. 2021

JCM

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Bacteremia—i.e., the presence of pathogens in the blood stream—is associated with long-term morbidity and is a potential precursor condition to life-threatening sepsis. Timely detection of bacteremia is therefore critical to reduce patient mortality, but existing methods lack precision, speed, and sensitivity to effectively stratify bacteremic patients. Herein, we tested the potential of quantitative serum N-glycomics performed using porous graphitized carbon liquid chromatography tandem mass spectrometry to stratify bacteremic patients infected with Escherichia coli (n = 11), Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from healthy donors (n = 39). In total, 62 N-glycan isomers spanning 41 glycan compositions primarily comprising complex-type core fucosylated, bisecting N-acetylglucosamine (GlcNAc), and α2,3-/α2,6-sialylated structures were profiled across all samples using label-free quantitation. Excitingly, unsupervised hierarchical clustering and principal component analysis of the serum N-glycome data accurately separated the patient groups. P. aeruginosa-infected patients displayed prominent N-glycome aberrations involving elevated levels of fucosylation and bisecting GlcNAcylation and reduced sialylation relative to other bacteremic patients. Notably, receiver operating characteristic analyses demonstrated that a single N-glycan isomer could effectively stratify each of the four bacteremic patient groups from the healthy donors (area under the curve 0.93–1.00). Thus, the serum N-glycome represents a new hitherto unexplored class of potential diagnostic markers for bloodstream infections.

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Pseudomonas aeruginosa bacteremia in patients with liver cirrhosis: a comparison with bacteremia caused by Enterobacteriaceae

Cited by 9 publications

References 25 publications

Selective Inactivation ofPseudomonas aeruginosaandStaphylococcus epidermidiswith Pulsed Electric Fields and Antibiotics

Selective Inactivation ofPseudomonas aeruginosaandStaphylococcus epidermidiswith Pulsed Electric Fields and Antibiotics

Expected and Unexpected Products from the Biochemical Oxidation of Bacterial Alkylquinolones with CYP4F11

Serum N-Glycomics Stratifies Bacteremic Patients Infected with Different Pathogens

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