Pseudomonas aeruginosa alginate is a potent secretagogue in the isolated ferret trachea

Kishioka, Chikako; Okamoto, Kosuke; Hassett, Daniel J.; Mello, Daphne de; Rubin, Bruce K.

doi:10.1002/(sici)1099-0496(199903)27:3<174::aid-ppul4>3.0.co;2-a

Cited by 19 publications

(13 citation statements)

References 20 publications

(35 reference statements)

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“…Therefore, its population can be divided into many subsets depending on which epitopes of mucins are measured. Three methods are currently used: the gel-filtration method, lectin assay (49), and immunoassay (ELISA). Comparison of these methods is briefly described elsewhere (50).…”

Section: Significance Of the Results From The Pharmacological Studiesmentioning

confidence: 99%

Pharmacology of Airway Goblet Cell Mucin Release

et al. 2003

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Abstract. Airway mucus hypersecretion is one of the major clinical manifestations of patients suffering from various pulmonary diseases. However, no drugs are yet available to control airway mucus hypersecretion. For the past 15 years, a plethora of information has amassed with regard to the pharmacology of airway goblet cell mucin secretion using various primary cell culture systems. The recent discovery of various MUC genes has also greatly stimulated research in this field. Nevertheless, the heterogeneity of the oligosaccharide structure of mucin molecules makes it extremely difficult to assess the significance of the information derived from the pharmacological studies. Therefore, it seems crucially important to understand the roles of individual mucins in conjunction with normal airway physiology and pathology, which should be the future research directions necessary for studying airway goblet cell mucin pharmacology.

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Section: Significance Of the Results From The Pharmacological Studiesmentioning

confidence: 99%

Pharmacology of Airway Goblet Cell Mucin Release

et al. 2003

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“…A small, uncharacterized secreted factor (Ͼ3 kDa) produced by P. aeruginosa has also been shown to suppress interleukin-8 and NF-B expression from epithelial cells (40), thus damping the typical inflammatory response to pathogens. Along with these extracellular proteins, production of the polysaccharide alginate increases in many CF strains, which in turn stimulates mucin production, thereby limiting immune recognition and clearance (41)(42)(43).…”

Section: Infection Maintenancementioning

confidence: 95%

“…2) (52). We believe this epoxide hydrolase activity is important for the Cif-mediated effect on CFTR, as a mutation just outside the active-site tunnel eliminates the epithelial cell activity of Cif (41,44). Ongoing studies are aimed at verifying this hypothesis.…”

Section: Cif a Novel Virulence Factormentioning

confidence: 99%

Pouring Salt on a Wound: Pseudomonas aeruginosa Virulence Factors Alter Na+ and Cl- Flux in the Lung

Ballok

O’Toole

2013

Journal of Bacteriology

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Pseudomonas aeruginosa is a ubiquitous opportunistic pathogen with multiple niches in the human body, including the lung. P. aeruginosa infections are particularly damaging or fatal for patients with ventilator-associated pneumonia, chronic obstructive pulmonary disease, and cystic fibrosis (CF). To establish an infection, P. aeruginosa relies on a suite of virulence factors, including lipopolysaccharide, phospholipases, exoproteases, phenazines, outer membrane vesicles, type III secreted effectors, flagella, and pili. These factors not only damage the epithelial cell lining but also induce changes in cell physiology and function such as cell shape, membrane permeability, and protein synthesis. While such virulence factors are important in initial infection, many become dysregulated or nonfunctional during the course of chronic infection. Recent work on the virulence factors alkaline protease (AprA) and CF transmembrane conductance regulator inhibitory factor (Cif) show that P. aeruginosa also perturbs epithelial ion transport and osmosis, which may be important for the long-term survival of this microbe in the lung. Here we discuss the literature regarding host physiology-altering virulence factors with a focus on Cif and AprA and their potential roles in chronic infection and immune evasion.

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“…Third, amino acid identity between ferret and human nucleotide binding domain 1 (NBD1) of CFTR is a striking 97% [10], which is just as high as nonhuman primates (96%, Macaca nemestrina) and significantly greater than rodents (80%, rat and mouse). Finally, the domestic ferret has proven useful for the study of human infectious lung disease due to the similarity between ferret and human lung biology [12][13][14][15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Developmental Capacity of Ferret Embryos by Nuclear Transfer Using G0/G1-Phase Fetal Fibroblasts1

Li¹,

Sabet²,

Zhou³

et al. 2003

Biology of Reproduction

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With the ultimate goal of establishing experimental protocols necessary for cloning ferrets, the present study has established parameters for the reconstruction of ferret embryos by nuclear transfer (NT) using G0/G1-phase donor fetal fibroblasts. Cumulus-oocyte complexes were harvested from superovulated ferrets and cultured in maturation medium for 24 h. Matured oocytes were then enucleated and injected with the fibroblast nuclei derived from 14-16-h serum-starved cells. Reconstructed embryos were then activated by a combination of electric pulses and chemical stimulations. Subsequently, the reconstructed and activated embryos were either cultured in vitro or transferred to pseudopregnant ferrets to evaluate their developmental capacity in vitro and in vivo. Our results demonstrated that 56.3% of reconstructed embryos (n = 187) cleaved, while 26.0% and 17.6% developed to morula and blastocyst phases in vitro, respectively. The blastocysts derived from NT embryos demonstrated normal morphology by differentially staining as compared to normal blastocysts developed in vivo following fertilization. In vivo developmental studies at 21 days posttransplantation demonstrated 8.8% of reconstructed embryos (n = 91) implanted into the uterine lining of recipients, while 3.3% formed fetuses. However, reconstructed embryos (n = 387) failed to develop to term (42 days). These results demonstrate donor nuclei of G0/G1-phase fetal fibroblast cells can be reprogrammed to support the development of reconstructed ferret embryos in vitro and in vivo; however, a significant third-trimester block occurs preventing full-term development.

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Pseudomonas aeruginosa alginate is a potent secretagogue in the isolated ferret trachea

Cited by 19 publications

References 20 publications

Pharmacology of Airway Goblet Cell Mucin Release

Pharmacology of Airway Goblet Cell Mucin Release

Pouring Salt on a Wound: Pseudomonas aeruginosa Virulence Factors Alter Na+ and Cl- Flux in the Lung

Developmental Capacity of Ferret Embryos by Nuclear Transfer Using G0/G1-Phase Fetal Fibroblasts1

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