Developmental Capacity of Ferret Embryos by Nuclear Transfer Using G0/G1-Phase Fetal Fibroblasts1

Li, Ziyi; Sabet, Maryam Rezaei; Zhou, Qi; Liu, Xiaoming; Ding, Wei; Renard, Jean‐Paul; Engelhardt, John F.

doi:10.1095/biolreprod.102.012369

Cited by 12 publications

(10 citation statements)

References 35 publications

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“…3 The number of cleaved embryos or blastocysts was divided by the number of fused embryos. nuclear transfer recipient [12,13], relatively little information is available regarding the fusion of enucleated oocytes with donor cells. SLO is known to form pores in the plasma membranes of mammalian cells and has been used to deliver proteins into T-cells through those pores [5,6].…”

Section: Discussionmentioning

confidence: 99%

Streptolysin-O Treatment of Fetal Fibroblasts Improves Cell Fusion and In Vitro Development of Porcine Nuclear Transfer Embryos

Naruse

Quan

Kim

et al. 2009

Journal of Reproduction and Development

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Abstract. Streptolysin O (SLO) is a known bacterial protein that forms very large pores in the plasma membranes of mammalian cells. SLO has been used in the delivery of proteins into living cells following permeabilization. The objective of this study was to investigate the effects of SLO treatment in donor cells that generate pores in the plasma membrane during in vitro development of porcine reconstructed embryos. In experiment 1, fetal fibroblast cells were trypsinized, treated with SLO for 0, 30, 50 and 70 min and then their fusion rates and developmental capabilities were tested following reconstruction. SLO treatment for 50 min produced a higher blastocyst formation rate compared with the other treatments. In experiment 2, fetal fibroblasts were treated with 200 ng/ml SLO for 50 min at the confluent or single-cell stages of the nuclear transfer protocol (i.e., before and after trypsinization, respectively), and the in vitro development of the resulting porcine reconstructed embryos was investigated over 6 days in culture. Oocytes receiving cells treated with SLO post-trypsinization showed higher fusion and blastocyst formation rates compared with those receiving untreated cells. Collectively, these findings show that SLO-mediated permeabilization of porcine fetal fibroblast cells appears to improve the fusion rates and in vitro development of porcine reconstructed embryos.

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Section: Discussionmentioning

confidence: 99%

Streptolysin-O Treatment of Fetal Fibroblasts Improves Cell Fusion and In Vitro Development of Porcine Nuclear Transfer Embryos

Naruse

Quan

Kim

et al. 2009

Journal of Reproduction and Development

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“…It is the color pattern requested by most researchers, in contrast with the many coat color variations that abound in the pet industry. One research study, however, reported the use of albino ferrets as pseudopregnant recipients of embryos from cinnamon-colored parents (Li et al 2003).…”

Section: Research-related Ferret Selection Criteria and Biologymentioning

confidence: 99%

Issues to Consider for Preparing Ferrets as Research Subjects in the Laboratory

Ball¹

2006

ILAR Journal

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The domestic or European ferret (Mustela putorius furo) has been domesticated for thousands of years. Ferrets have been used for hunting and fur production, as pets, and as models in biomedical research. Despite the relatively small numbers used in the laboratory, ferrets have some unique applications including study of human influenza and severe acute respiratory syndrome (SARS)-associated corona virus. They have served as models for peptic ulcer disease, carotenoid metabolism, cystic fibrosis, and drug emesis screening, among others. Most research ferrets are males, due to estrus-related health problems in females. They may be housed conventionally and are easy to care for when their biology and behavior are understood. Due to the small number of ferret suppliers, animals are often shipped long distances, requiring air transport and intermediate handlers. It is important to minimize shipment stress, especially with weanling and pregnant animals. Additional expertise is required for success with pregnant and whelping ferrets and for rearing of neonates. The animals have specific dietary requirements, and proper nutrition is key. Successful housing requires knowledge of ferret behaviors including social behavior, eating habits, a general inquisitive nature, and a species-typical need to burrow and hide. Regular handling is necessary to maintain well-being. A ferret health care program consists of physical examination, immunization, clinical pathology, and a working knowledge of common ferret diseases. Various research methodologies have been described, from basic procedures such as blood collection to major invasive survival surgery. Ferrets have a distinct niche in biomedical research and are hardy animals that thrive well in the laboratory.

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“…Over the past few years, several of the critical steps required for NT cloning in the ferret have been established [33-35]. This review will focus on progress in the various steps highlighted in Figure 2.…”

Section: Strategy and Progress In Cloning A Cf Ferretmentioning

confidence: 99%

“…Progress in each of the boxed methodologies will be discussed in detail in subsequent sections [33-35]. Italicized labels indicate descriptive markings, not methodological steps per se.…”

Section: Strategy and Progress In Cloning A Cf Ferretmentioning

confidence: 99%

Progress toward generating a ferret model of cystic fibrosis by somatic cell nuclear transfer

Engelhardt

2003

Reprod Biol Endocrinol

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Mammalian cloning by nuclear transfer from somatic cells has created new opportunities to generate animal models of genetic diseases in species other than mice. Although genetic mouse models play a critical role in basic and applied research for numerous diseases, often mouse models do not adequately reproduce the human disease phenotype. Cystic fibrosis (CF) is one such disease. Targeted ablation of the cystic fibrosis transmembrane conductance regulator (CFTR) gene in mice does not adequately replicate spontaneous bacterial infections observed in the human CF lung. Hence, several laboratories are pursuing alternative animal models of CF in larger species such as the pig, sheep, rabbits, and ferrets. Our laboratory has focused on developing the ferret as a CF animal model. Over the past few years, we have investigated several experimental parameters required for gene targeting and nuclear transfer (NT) cloning in the ferret using somatic cells. In this review, we will discuss our progress and the hurdles to NT cloning and gene-targeting that accompany efforts to generate animal models of genetic diseases in species such as the ferret.

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Developmental Capacity of Ferret Embryos by Nuclear Transfer Using G0/G1-Phase Fetal Fibroblasts1

Cited by 12 publications

References 35 publications

Streptolysin-O Treatment of Fetal Fibroblasts Improves Cell Fusion and In Vitro Development of Porcine Nuclear Transfer Embryos

Streptolysin-O Treatment of Fetal Fibroblasts Improves Cell Fusion and In Vitro Development of Porcine Nuclear Transfer Embryos

Issues to Consider for Preparing Ferrets as Research Subjects in the Laboratory

Progress toward generating a ferret model of cystic fibrosis by somatic cell nuclear transfer

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