PSA detection using label free graphene FET with coplanar electrodes based microfluidic point of care diagnostic device

Mandal, Naresh; Pakira, Victor; Samanta, N.; Das, Naren; Chakraborty, Suman; Pramanick, Bidhan; RoyChaudhuri, C.

doi:10.1016/j.talanta.2020.121581

Cited by 32 publications

(15 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The BSA|Ab|TGA-OsTe 2 QD-PPy immunosensor exhibits improved sensing characteristics such as a linear range of 0-15 ng/mL (i.e., the concentration range studied was linear) and the limit of detection value of 0.36 ng/mL. The linear range and the limit of detection values of the immunosensor cover the physiologically relevant PSA levels (0-14 ng/mL) [60] for ascertaining prostate cancer related health conditions. The immunosensor was constructed with a highly selective anti-PSA antibody, which was shown in previous work [61] not to exhibit false positive signal in the presence of other proteins (i.e., interferences), such as alpha fetal protein (AFP), carcinoembryonic antigen (CEA), human chorionic gonadotropin (HCG), immunoglobulin G (IgG) and thrombin.…”

Section: Discussionmentioning

confidence: 99%

Biocompatible Osmium Telluride-Polypyrrole Nanocomposite Material: Application in Prostate Specific Antigen Immunosensing

2021

View full text Add to dashboard Cite

Prostate cancer is a dominant global threat to society. It affects nearly 4000 men in South Africa annually, making it the second most threatening cancerous disease after lung cancer. A potential serological biomarker to monitor early diagnosis of prostate cancer is prostate specific antigen (PSA). We used the PSA biomarker in our work to develop an extremely sensitive electrochemical immunosensor to achieve low detection limits. The fabrication steps followed with the combination of thioglycolic acid capped osmium telluride quantum dots (TGA-OsTe2QD)-polypyrrole (PPy) nanocomposite and prostate specific antigen modified on a glassy carbon electrode. The UV-Vis signatures of TGA-OsTe2QD-PPy showed an absorption band at 262 nm which is attributed to the PPy and TGA-OsTe2QD composite. This band corresponds to the energy band gap of 4.4 and 5.4 eV. The CV responses of BSA|Ab|TGA-OsTe2QD|PPy|GCE modified electrode to prostate specific antigen (PSA) was studied within a range of 0–16 ng/mL PSA that was linear, herein referred to as liner range (LR), which produced a limit of detection (LOD) value of 0.36 ng/mL PSA. The values of the immunosensor’s calibration parameters (LR and LOD) make them suitable for real sample application, due to their coverage of the PSA concentration range (0–14 ng/mL) that is of clinical importance.

show abstract

Section: Discussionmentioning

confidence: 99%

Biocompatible Osmium Telluride-Polypyrrole Nanocomposite Material: Application in Prostate Specific Antigen Immunosensing

2021

View full text Add to dashboard Cite

show abstract

“…Microfluidic technology has shown great promise for portable, low-cost, and rapid quantitative detection in fluid samples such as suspended cells and particles in a small volume (in the range of μL to pL) without sample preparation steps for POC diagnostics . In particular, integration of the GFET biosensor with digital or droplet microfluidics has become a mature technology in the design of portable, single-platform, digital biosensing for sensitive detection of biomolecules and diagnosis of associated diseases. , For example, Khan et al fabricated a GFET-integrated portable microfluidic device for sensitive and real-time detection of thrombin biomarkers. The integrated device consisted of a microfluidic channel with an inlet and an outlet that traverses the source, drain, and an in-plane gate electrode.…”

Section: Gfet Biosensors For Biomarker Detectionmentioning

confidence: 99%

Graphene-Based Field-Effect Transistors in Biosensing and Neural Interfacing Applications: Recent Advances and Prospects

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Therefore, if a device could quickly and cost-effectively detect PSA indices lower than 4 ng/mL, the diagnosis of prostate cancer patients could be improved [165]. Mandal et al, designed a system integrating dielectrophoresis (DEP), graphene FETs, and a compact disc-based microfluidic to detect PSA content of less than 4 ng/mL in blood serum, thus this could be used for the early screening for prostate cancer [166].…”

Section: Applications For Cancer Detectionmentioning

confidence: 99%

Microfluidic Point-of-Care (POC) Devices in Early Diagnosis: A Review of Opportunities and Challenges

Yang

Zhang

et al. 2022

Sensors

104

View full text Add to dashboard Cite

The early diagnosis of infectious diseases is critical because it can greatly increase recovery rates and prevent the spread of diseases such as COVID-19; however, in many areas with insufficient medical facilities, the timely detection of diseases is challenging. Conventional medical testing methods require specialized laboratory equipment and well-trained operators, limiting the applicability of these tests. Microfluidic point-of-care (POC) equipment can rapidly detect diseases at low cost. This technology could be used to detect diseases in underdeveloped areas to reduce the effects of disease and improve quality of life in these areas. This review details microfluidic POC equipment and its applications. First, the concept of microfluidic POC devices is discussed. We then describe applications of microfluidic POC devices for infectious diseases, cardiovascular diseases, tumors (cancer), and chronic diseases, and discuss the future incorporation of microfluidic POC devices into applications such as wearable devices and telemedicine. Finally, the review concludes by analyzing the present state of the microfluidic field, and suggestions are made. This review is intended to call attention to the status of disease treatment in underdeveloped areas and to encourage the researchers of microfluidics to develop standards for these devices.

show abstract

PSA detection using label free graphene FET with coplanar electrodes based microfluidic point of care diagnostic device

Cited by 32 publications

References 31 publications

Biocompatible Osmium Telluride-Polypyrrole Nanocomposite Material: Application in Prostate Specific Antigen Immunosensing

Biocompatible Osmium Telluride-Polypyrrole Nanocomposite Material: Application in Prostate Specific Antigen Immunosensing

Graphene-Based Field-Effect Transistors in Biosensing and Neural Interfacing Applications: Recent Advances and Prospects

Microfluidic Point-of-Care (POC) Devices in Early Diagnosis: A Review of Opportunities and Challenges

Contact Info

Product

Resources

About