Biocompatible Osmium Telluride-Polypyrrole Nanocomposite Material: Application in Prostate Specific Antigen Immunosensing

Abstract

Prostate cancer is a dominant global threat to society. It affects nearly 4000 men in South Africa annually, making it the second most threatening cancerous disease after lung cancer. A potential serological biomarker to monitor early diagnosis of prostate cancer is prostate specific antigen (PSA). We used the PSA biomarker in our work to develop an extremely sensitive electrochemical immunosensor to achieve low detection limits. The fabrication steps followed with the combination of thioglycolic acid capped osmium telluride quantum dots (TGA-OsTe2QD)-polypyrrole (PPy) nanocomposite and prostate specific antigen modified on a glassy carbon electrode. The UV-Vis signatures of TGA-OsTe2QD-PPy showed an absorption band at 262 nm which is attributed to the PPy and TGA-OsTe2QD composite. This band corresponds to the energy band gap of 4.4 and 5.4 eV. The CV responses of BSA|Ab|TGA-OsTe2QD|PPy|GCE modified electrode to prostate specific antigen (PSA) was studied within a range of 0–16 ng/mL PSA that was linear, herein referred to as liner range (LR), which produced a limit of detection (LOD) value of 0.36 ng/mL PSA. The values of the immunosensor’s calibration parameters (LR and LOD) make them suitable for real sample application, due to their coverage of the PSA concentration range (0–14 ng/mL) that is of clinical importance.

Futuristic Silicon Photonic Biosensor with Nanomaterial Enhancement for PSA Detection

“…Table 1 shows the proposed method with already reported PSA immunosensors. The LOD values reported by Gupta et al [52], Oliveira et al [57], and Jang et al [58] are 3.6 x 10 -1 , 5.9 x 10 -1 ng/mL and 1.18 ng/mL, respectively. These reported values are respectively 2.1, 3.5 and 6.9 orders of magnitude higher than the LOD obtained in this current work.…”

Futuristic Silicon Photonic Biosensor with Nanomaterial Enhancement for PSA Detection

“…Table 1 shows the proposed method with already reported PSA immunosensors. The LOD values reported by Gupta et al [52], Oliveira et al [57], and Jang et al [58] are 3.6 × 10 −1 , 5.9 × 10 −1 ng/mL and 1.18 ng/mL, respectively. These reported values are, respectively, 2.1, 3.5, and 6.9 orders of magnitude higher than the LOD obtained in this current work.…”