Precisely designed growth of dual-color quantum dots bilayer nanobeads for ratiometric fluorescent immunoassay

Fan, Jinjin; Li, Ning; Wang, Fangfang; Lv, Yanbing; Jin, Qiaoli; Zhao, Man; Shi, Yangchao; Wu, Ruili; Shen, Huaibin; Li, Lin Song

doi:10.1016/j.snb.2022.132888

Cited by 5 publications

(2 citation statements)

References 32 publications

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“…Quantum dots (QDs) are ideal optical labels for highly sensitive multiple detection in in vitro diagnostics (IVD) due to their unique optical properties. 1,2 Optical biosensors based on QDs have been widely used to detect a wide range of biomarkers, small molecule toxins, etc. 3−6 The accuracy and sensitivity of obtaining more biological information from low volume will be the future trend in the development of quantitative detections in IVD, 7,8 especially for highly sensitive and disease-specific biomarker detection, such as those associated with infectious diseases.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Quantum dots (QDs) are ideal optical labels for highly sensitive multiple detection in in vitro diagnostics (IVD) due to their unique optical properties. , Optical biosensors based on QDs have been widely used to detect a wide range of biomarkers, small molecule toxins, etc . − The accuracy and sensitivity of obtaining more biological information from low volume will be the future trend in the development of quantitative detections in IVD, , especially for highly sensitive and disease-specific biomarker detection, such as those associated with infectious diseases . A delayed early diagnosis and timely treatment of infectious diseases can result in sepsis, multiple organ dysfunction syndrome (MODS), and even life-threatening disease progression. − Procalcitonin (PCT) and Interleukin-6 (IL-6), among the many highly sensitive and specific inflammatory factors, are new effective indexes for assessing the severity of bacterial infection, determining the prognosis of the disease, evaluating the effectiveness of antibiotic use, and guiding clinical treatment .…”

Section: Introductionmentioning

confidence: 99%

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Development of a Dual Fluorescence Signal-Enhancement Immunosensor Based on Substrate Modification for Simultaneous Detection of Interleukin-6 and Procalcitonin

Zhao,

Yang,

et al. 2024

Langmuir

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High-sensitivity detection of biomarkers is of great significance to improve the accuracy of disease diagnosis and the rate of occult disease diagnosis. Using a substrate modification and two-color quantum dot (QD) nanobeads (QBs), we have developed a dual fluorescence signal-enhancement immunosensor for sensitive, simultaneous detection of interleukin 6 (IL-6) and procalcitonin (PCT) at low volumes (∼20 μL). First, the QBs compatible with QDs with different surface ligands were prepared by optimizing surfactants based on the microemulsion method. Through the use of a fluorescence-linked immunosorbent assay (FLISA), the feasibility of a dual signal-enhancement immunosensor was verified, and a 5-fold enhancement of fluorescence intensity was achieved after the directional coating of the antibodies on sulfhydryl functionalization (−SH) substrates and the preparation of QBs by using a polymer and silica double-protection method. Next, a simple polydimethylsiloxane (HS-PDMS) immunosensor with a low volume consumption was prepared. Under optimal conditions, we achieved the simultaneous detection of IL-6 and PCT with a linear range of 0.05−50 ng/mL, and the limit of detection (LOD) was 24 and 32 pg/ mL, respectively. The result is comparable to two-color QBs-FLISA with a sulfhydryl microplate, even though only 20% of its volume was used. Thus, the dual fluorescence signal-enhancement HS-PDMS immunosensor offers the capability of early microvolume diagnosis of diseases, while the detection of inflammatory factors is clinically important for assisting disease diagnosis and determining disease progression.

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