PRQFVamide, a Novel Pentapeptide Identified From the CNS and Gut of<i>Aplysia</i>

Furukawa, Yasuo; Nakamaru, K.; Sasaki, Ken−ichi; Fujisawa, Yuko; Minakata, Hiroyuki; Ohta, Shigeru; Morishita, Fumihiro; Matsushima, Osamu; Li, L.; Alexeeva, Vera; Ellis, Timothy A.; Dembrow, Nikolai C.; Jing, Jian; Sweedler, Jonathan V.; Weiss, Klaudiusz R.; Vilim, Ferdinand S.

doi:10.1152/jn.00014.2003

Cited by 39 publications

(36 citation statements)

References 55 publications

(57 reference statements)

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“…unpaired neuron in the buccal ganglion (Furukawa et al 2003). Here, we have characterized this neuron's morphology, electrophysiological properties, and connections to the feeding circuitry.…”

Section: Discussionmentioning

confidence: 99%

“…Immunocytochemistry was performed using the previously characterized rat anti-PRQFVa antibody (Furukawa et al 2003), following the previously described protocol (Vilim et al 1996). Ganglia were fixed (4% paraformaldehyde, 0.2% picric acid, 25% sucrose, 0.1 M NaH 2 PO 4 , pH 7.6) overnight at 4°C.…”

Section: Immunocytochemistrymentioning

confidence: 99%

“…A recent study mapping the distribution of a newly isolated and cloned neuropeptide PRQFVamide (PRQFVa) revealed an unpaired neuron on the caudal surface of the buccal ganglion (Furukawa et al 2003). Because no unpaired neurons had been identified in that region of the buccal ganglion (Church and Lloyd 1994;Evans and Cropper 1998;Goldstein and Schwartz 1989;Kabotyanski et al 1998), we hypothesized that the PRQFVa-positive neuron might be a heretofore-unidentified CPG element.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, some interneurons can modify the duration of specific phases in the motor programs (Evans and Cropper 1998;Hurwitz et al 2003;Jing et al 2003). Despite the progress made toward characterizing the functions of identified CPG elements, the current description of the buccal CPG is incomplete and important interneurons remain to be identified.A recent study mapping the distribution of a newly isolated and cloned neuropeptide PRQFVamide (PRQFVa) revealed an unpaired neuron on the caudal surface of the buccal ganglion (Furukawa et al 2003). Because no unpaired neurons had been identified in that region of the buccal ganglion (Church and Lloyd 1994;Evans and Cropper 1998;Goldstein and Schwartz 1989;Kabotyanski et al 1998), we hypothesized that the PRQFVa-positive neuron might be a heretofore-unidentified CPG element.…”

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A Newly Identified Buccal Interneuron Initiates and Modulates Feeding Motor Programs inAplysia

Dembrow

Jing

Proekt

et al. 2003

Journal of Neurophysiology

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. A newly identified buccal interneuron initiates and modulates feeding motor programs in Aplysia. J Neurophysiol 90: 2190 -2204. First published June 11, 2003 10.1152/jn.00173.2003. Despite considerable progress in characterizing the feeding central pattern generator (CPG) in Aplysia, the full complement of neurons that generate feeding motor programs has not yet been identified. The distribution of neuropeptide-containing neurons in the buccal and cerebral ganglia can be used as a tool to identify additional elements of the feeding circuitry by providing distinctions between otherwise morphologically indistinct neurons. For example, our recent study revealed a unique and potentially interesting unpaired PRQFVamide (PRQFVa)-containing neuron in the buccal ganglion. In this study, we describe the morphological and electrophysiological characterization of this novel neuron, which we designate as B50. We found that activation of B50 is capable of producing organized rhythmic output of the feeding CPG. The motor programs elicited by B50 exhibit some similarities as well as differences to motor programs elicited by the command-like cerebral-to-buccal interneuron CBI-2. In addition to activating the feeding CPG, B50 may act as a program modulator. I N T R O D U C T I O NThe feeding motor system of Aplysia is a useful preparation for the study of both the central and the peripheral mechanisms underlying control of rhythmic motor behavior. This system produces a number of ingestive or egestive feeding behaviors, which can be distinguished by phasing and duration of protraction-retraction and opening-closing movements of the radula, the main feeding apparatus of Aplysia (Kupfermann 1974; Morton and Chiel 1993a,b). Although many of the neurons involved in central control of the feeding system have been identified (see following text), the full complement of neurons involved in the generation of feeding motor programs has not yet been identified.The output of feeding motor programs is organized by a central pattern generator (CPG) that is located in the buccal ganglion (e.g., Hurwitz et al. 1997Hurwitz et al. , 2003Susswein and Byrne 1988). Activity in the buccal CPG is initiated and modulated by higher-order command-like neurons located in the cerebral ganglion (Hurwitz et al. 2003;Jing and Weiss 2001;Morgan et al. 2000Morgan et al. , 2002Rosen et al. 1991;Xin et al. 1999). A combination of semi-intact preparations and chronic nerve recordings in behaving animals has enabled correlation of patterned outputs (motor programs) generated in the isolated CNS with several of the feeding-related behaviors in the intact animal (Cropper et al. 1990;Kupfermann and Weiss 1982;Morgan et al. 2002; Morton and Chiel 1993a,b;Rosen et al. 1991). To understand how the buccal CPG generates such coordinated and yet flexible motor programs, the role of the various buccal CPG elements must be elucidated. To this end, the contributions of several identified interneurons to the output of the feeding CPG have been characterized (e.g., Brembs ...

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Section: Discussionmentioning

confidence: 99%

Section: Immunocytochemistrymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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A Newly Identified Buccal Interneuron Initiates and Modulates Feeding Motor Programs inAplysia

Dembrow

Jing

Proekt

et al. 2003

Journal of Neurophysiology

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“…18. , c: PRQFVamide 27) , d: AMRP 13) , e: myomodulin 22) , f: enterin 14) , g: buccalin 21) , h: FCAP 29) . The length of the peptides for a-h are 4, 4, 5, 6/7, 7/8, 9/10, 10-12, and 12/13, respectively.…”

Section: 松島治unclassified

Structure and Physiological Action of Novel Multiple Family Peptides of Aplysia

et al. 2006

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松島治Neuropeptides are perhaps the most diverse intercellular signaling molecules in the animal kingdom. Owing to the high-throughput methodologies using the high-performance liquid chromatography as well as the mass spectrometry, the number of peptides identified from both vertebrates and invertebrates are increasing.In many cases, the multiple structurally related peptides which are unequivocally considered to be members of the same peptide family are found in the nervous system. We call such peptides the multiple family peptides. Here, we show the structure, distribution, and action of two kinds of multiple family peptides recently identified in a marine mollusc, Aplysia.

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Localization of biogenic amines in the foregut of Aplysia californica: Catecholaminergic and serotonergic innervation

Martinez-Rubio

Serrano

2009

J of Comparative Neurology

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This study examined the catecholaminergic and serotonergic innervation of the foregut of Aplysia californica, a model system in which the control of feeding behaviors can be investigated at the cellular level. Similar numbers (15-25) of serotonin-like-immunoreactive (5HTli) and tyrosine hydroxylase-like-immunoreactive (THli) fibers were present in each (bilateral) esophageal nerve (En), the major source of pregastric neural innervation in this system. The majority of En 5HTli and THli fibers originated from the anterior branch (En2), which innervates the pharynx and the anterior esophagus. Fewer fibers were present in the posterior branch (En1), which innervates the majority of the esophagus and the crop. Backfills of the two En branches toward the central nervous system (CNS) labeled a single, centrifugally projecting serotonergic fiber, originating from the metacerebral cell (MCC). The MCC fiber projected only to En2. No central THli neurons were found to project to the En. Surveys of the pharynx and esophagus revealed major differences between their patterns of catecholaminergic (CA) and serotonergic innervation. Whereas THli fibers and cell bodies were distributed throughout the foregut, 5HTli fibers were present in restricted plexi, and no 5HTli somata were detected. Double-labeling experiments in the periphery revealed THli neurons projecting toward the buccal ganglion via En2. Other afferents received dense perisomatic serotonergic innervation. Finally, qualitative and quantitative differences were observed between the buccal motor programs (BMPs) produced by stimulation of the two En branches. These observations increase our understanding of aminergic contributions to the pregastric regulation of Aplysia feeding behaviors.

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PRQFVamide, a Novel Pentapeptide Identified From the CNS and Gut ofAplysia

Cited by 39 publications

References 55 publications

A Newly Identified Buccal Interneuron Initiates and Modulates Feeding Motor Programs inAplysia

A Newly Identified Buccal Interneuron Initiates and Modulates Feeding Motor Programs inAplysia

Structure and Physiological Action of Novel Multiple Family Peptides of Aplysia

Localization of biogenic amines in the foregut of Aplysia californica: Catecholaminergic and serotonergic innervation

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