PROXIMA 2A – A New Fully Tunable Micro-focus Beamline for Macromolecular Crystallography

Duran, Denis; Couster, Sebastien Le; Desjardins, Kewin; Delmotte, A.; Fox, Gavin C.; Meijers, Rob; Moreno, T.; Savko, Martin; Shepard, William

doi:10.1088/1742-6596/425/1/012005

Cited by 29 publications

(27 citation statements)

References 13 publications

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“…Diffraction data were collected either (i) on beamline PXII (Fuchs et al, 2014) equipped with a PILATUS 6M detector or beamline PXIII (Bingel-Erlenmeyer et al, 2011) equipped with a MAR CCD or a PILATUS 2M-F detector at the Swiss Light Source (SLS), Villigen, Switzerland, (ii) on the PROXIMA-2A (PX2A) beamline (Duran et al, 2013) equipped with an EIGER X 9M detector at SOLEIL, Saint-Aubin, France or (iii) on beamline ID30B (McCarthy et al, 2018) equipped with a PILATUS3 6M detector at the ESRF, Grenoble, France. All serial data collections were performed at room temperature (T = 293-298 K) on crystals inside ChipX3, owing to the reduced scattering background of the chip (Pinker et al, 2013).…”

Section: X-ray Data Collection and Analysismentioning

confidence: 99%

A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography

Wijn

Hennig

Roche

et al. 2019

IUCrJ

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Determining optimal conditions for the production of well diffracting crystals is a key step in every biocrystallography project. Here, a microfluidic device is described that enables the production of crystals by counter-diffusion and their direct on-chip analysis by serial crystallography at room temperature. Nine `non-model' and diverse biomacromolecules, including seven soluble proteins, a membrane protein and an RNA duplex, were crystallized and treated on-chip with a variety of standard techniques including micro-seeding, crystal soaking with ligands and crystal detection by fluorescence. Furthermore, the crystal structures of four proteins and an RNA were determined based on serial data collected on four synchrotron beamlines, demonstrating the general applicability of this multipurpose chip concept.

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Section: X-ray Data Collection and Analysismentioning

confidence: 99%

A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography

Wijn

Hennig

Roche

et al. 2019

IUCrJ

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“…Unfortunately, the initial data set collected on ID23-1 for the 2c complex could not be refined with good statistics, probably because of superposition of diffraction from microcrystals of the poorly solubilized ligand. It was recollected on beamline Proxima-2A 35 at the Soleil storage ring in St. Aubin, France. To recollect the data, crystals that had dried out after 18-month storage were re-hydrated by the addition of 300 mL of water to the sitting drop reservoir.…”

Section: Crystal Preparation and Structure Determinationmentioning

confidence: 99%

Synthesis and structural analysis of halogen substituted fibril formation inhibitors of Human Transthyretin (TTR)

Ciccone

Nencetti

Rossello

et al. 2016

Journal of Enzyme Inhibition and Medicinal Chemistry

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Transthyretin (TTR), a β-sheet-rich tetrameric protein, in equilibrium with an unstable amyloidogenic monomeric form is responsible for extracellular deposition of amyloid fibrils, is associated with the onset of neurodegenerative diseases, such as senile systemic amyloidosis, familial amyloid polyneuropathy and familial amyloid cardiomyopathy. One of the therapeutic strategies is to use small molecules to stabilize the TTR tetramer and thus curb amyloid fibril formation. Here, we report the synthesis, the in vitro evaluation of several halogen substituted 9-fluorenyl- and di-benzophenon-based ligands and their three-dimensional crystallographic analysis in complex with TTR. The synthesized compounds bind TTR and stabilize the tetramer with different potency. Of these compounds, 2c is the best inhibitor. The dual binding mode prevalent in the absence of substitutions on the fluorenyl ring, is disfavored by (2,7-dichloro-fluoren-9-ylideneaminooxy)-acetic acid (1b), (2,7-dibromo-fluoren-9-ylideneaminooxy)-acetic acid (1c) and (E/Z)-((3,4-dichloro-phenyl)-methyleneaminooxy)-acetic acid (2c), all with halogen substitutions.

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“…In order to achieve both requirements, we recently developed a double surface bimorph mirror for the back-end horizontal beamline optics in combination with the secondary source beamline design. The same type of the double surface bimorph mirror was developed at the PROXIMA 2A beamline at SOLEIL for two different focusing configurations at one focal distance [2]. On the other hand, our mirror supplies three characteristic beams for the focal positions which are away from each other.…”

Section: Introductionmentioning

confidence: 99%

Newly designed double surface bimorph mirror for BL-15A of the photon factory

Igarashi¹,

Nitani²,

Takeichi³

et al. 2016

AIP Conference Proceedings

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PROXIMA 2A – A New Fully Tunable Micro-focus Beamline for Macromolecular Crystallography

Cited by 29 publications

References 13 publications

A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography

A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography

Synthesis and structural analysis of halogen substituted fibril formation inhibitors of Human Transthyretin (TTR)

Newly designed double surface bimorph mirror for BL-15A of the photon factory

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