Protostrongylus rufescens in goats: Morphological and molecular characterization

Macedo, Lucia Oliveira de; Miranda, Lucélia Fabiano de; Oliveira, Jéssica Cardoso Pessoa de; Ramos, Carlos Alberto do Nascimento; Verocai, Guilherme G.; Carvalho, Gílcia Aparecida de; Alves, Lêucio Câmara; Ramos, Rafael Antonio Nascimento

doi:10.1016/j.smallrumres.2019.11.005

Cited by 4 publications

(3 citation statements)

References 21 publications

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“…To date, there have been a few studies for identification of parasitic nematode juveniles 30 . Those usually involve measurement of some of the conventional characteristics such as length of sheath tails of different species of juvenile 31,32 . In order to investigate fluorescence properties of nematodes juveniles, we have performed a confocal experiment on Ascaris suum and Ascaris lumbricoides juveniles as shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Real-time detection and identification of nematode eggs genus and species through optical imaging

Qazi

Khalid

Poddar

et al. 2020

Sci Rep

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Nematode eggs are pervasive pathogens that infect billions of people and livestock every year. Adult parasitic nematode worms can be distinguished based on their size and morphology. However, their eggs, particularly their species Ascaris lumbricoides and Ascaris suum cannot be identified from each other. Identifying eggs of helminths from wastewater and sludge is important from a public health perspective to minimize the spread of Ascaris infections. Numerous methods exist for nematode identification, from a morphological-based approach to high throughput sequencing technology. However, these techniques are not consistent and often laborious and time-consuming. In this study, we demonstrate that non-invasive real-time identification of eggs is possible based on their intrinsic fluorescence. Using confocal microscopy, we investigate the autofluorescence properties of five species of nematode eggs and observe clear differences between genus and for the first time their species in sludge samples. This non-invasive imaging technique could lead to better understanding of these species and may assist in early control of diseases.

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Section: Resultsmentioning

confidence: 99%

Real-time detection and identification of nematode eggs genus and species through optical imaging

Qazi

Khalid

Poddar

et al. 2020

Sci Rep

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“…Symptoms of infection in wildlife are not visibly obvious, and differ from one host to another, and the health impacts vary from sporadic observance upon necropsy (Kabakci et al, 2007;Panayotova-Pencheva & Alexandrov, 2010) to massive associated mortality (Forrester, 1971;Demartini & Davies, 1977). Prevalent worldwide, protostrongylids are more widely studied in domestic ruminants (Kuchboev et al, 2017;Ahmadi et al, 2018;De Macedo et al, 2020) due to our economic interest in them, than in wild fauna. In the Mediterranean basin, several studies have been conducted in free-ranging wildlife, especially from the north shore: in wild caprinae from France, Spain, Italy and Portugal (Nocture et al, 1998;Acevedo et al, 2005;Cassini et al, 2015); in lagomorphs from France, Italy and Bulgaria (Lesage et al, 2014;Sergi et al, 2018;Panayotova-Pencheva et al, 2019), and in different species of ungulates from Iberian peninsula (Morrondo et al, 2017;Figueiredo et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Prevalent worldwide, protostrongylids are more widely studied in domestic ruminants ( Kuchboev et al ., 2017 ; Ahmadi et al ., 2018 ; De Macedo et al ., 2020 ) due to our economic interest in them, than in wild fauna. In the Mediterranean basin, several studies have been conducted in free-ranging wildlife, especially from the north shore: in wild caprinae from France, Spain, Italy and Portugal ( Nocture et al ., 1998 ; Acevedo et al ., 2005 ; Cassini et al ., 2015 ); in lagomorphs from France, Italy and Bulgaria ( Lesage et al ., 2014 ; Sergi et al ., 2018 ; Panayotova-Pencheva et al ., 2019 ), and in different species of ungulates from Iberian peninsula ( Morrondo et al ., 2017 ; Figueiredo et al ., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Coprological survey of protostrongylid infections in antelopes from Souss-Massa National Park (Morocco)

et al. 2020

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SummaryProtostrongylids, small nematode lungworms, are an integral part of the wild ruminant helminth community, which can damage animals’ health when they are held in captivity or semi-captive conditions. The Sahelo-Saharan antelope species dorcas gazelle (Gazella dorcas), the scimitar-horned oryx (Oryx dammah), and the addax (Addax nasomacculatus), reintroduced to Souss-Massa National Park in Morocco, could be host to many species of Protostrongylids. This study was conducted from January to July 2015 to identify infecting parasite species, and determine their prevalence and abundance in all three antelope species. A total of 180 individual fecal samples were collected, morphologically examined by the Baermann technique, and molecularly identified by PCR amplification and sequencing of the second internal transcribed spacer region of the rDNA (ITS-2).Two parasite species were found in the three antelope populations: Muellerius capillaris and Neostrongylus linearis. The prevalence scores recorded for M. capillaris were 98.40 % in the addax, 96.70 % in dorcas gazelle, and 28.40 % in the oryx. The prevalence rates of N. linearis were 60 % in the addax, 23.40 % in dorcas gazelle, and 90 % in the oryx. Excreted larvae were quantified by LPG (larvae per gram) counting: for M. capillaris, the LPG mean values were 92.94 in the addax, 133.09 in dorcas gazelle, and 1.48 in the oryx; and for N. linearis, the LPG mean values were 6.02 in the addax, 1.37 in dorcas gazelle, and 32.81 in the oryx. These findings indicate that the three species of antelopes are infected with Muellerius capillaris and Neostrongylus linearis to varying degrees in intensity and prevalence.

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Lungworms in ruminants from Brazil: A retrospective epidemiological study over four decades

Macedo

Lima

Verocai

et al. 2021

Veterinary Parasitology: Regional Studies and Reports

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Protostrongylus rufescens in goats: Morphological and molecular characterization

Cited by 4 publications

References 21 publications

Real-time detection and identification of nematode eggs genus and species through optical imaging

Real-time detection and identification of nematode eggs genus and species through optical imaging

Coprological survey of protostrongylid infections in antelopes from Souss-Massa National Park (Morocco)

Lungworms in ruminants from Brazil: A retrospective epidemiological study over four decades

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