Protoplasts of systemic dimorphic fungal pathogens: Histoplasma capsulatum and blastomyces dermatitidis

Berliner, Martha D.; Reca, Maria E.

doi:10.1007/bf02051335

Cited by 16 publications

(1 citation statement)

References 6 publications

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“…Enzyme degradation of some of the cell wall components facilitates more detailed immunological investigation. Fungal protoplasts have been prepared both from the yeast and mycelial stages of some causative agents of systemic mycoses, e. g. Histoplasma capsulatum and Blastomyces dermatitidis (1,2). Protoplasts from dermatophytes causing surface affections were first obtained by Koch and Lange (7).…”

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confidence: 99%

Protoplasts of Microsporum Gypseum Conidia:* Protoplasten der Konidien von Microsporum gypseum

Weigl

Hejtmánek

1979

Mycoses

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The technique of cell wall enzymatic lysis was used to prepare protoplastt from the microconidia of Microsporum gypseum. Cell wall elimination was immunologically verified. Protoplasts isolated from microconidia were found to be of the following two types: large with a vacuole and small without one. The small protoplasts regenerated on plates of osmotically stabilized complete medium with reduced agar content (0.5 O/o) and in stabilized liquid medium as well. The regeneration was discovered from the production of hyphae.* Herrn Prof. Dr. med. Dr. med. vet. 11. c. Hans Rieth zum 65. Geburtstag gewidmet. Das Tiefenantisepticum zur Lokaibehandlung bakterielier und mykoiischerAffektionen ! Mykohaug Plnselung Dermatomykosen (Epidermophytien), auch sekundar bakteriellinfizierte, rnykotische Ekreme (Interdigitalekzeme). Trychophytien Bestandteile: Bis-(2-rnethyl-4-arnlno-chinolyl-6)-carbarnid hydrochl. 0,0135 g. Acid. sal. 1.75 g. Aethanolarninborat 1 g, Acid. benz. 1,75 g. lsopropanol 50 g, Aqua dest. ad 100 g Handelsformen: Pinselflasche zu 30 rnl 4,60 DM, 50 rnl 6,35 DM und 100 rnl 10,lO DM rn. MwSt. SILWIE . CHEW. PHIRP. PRAPARITL , APOlHEKER MANFRED H I U C~ SWlTCRRl-WEDELFINEEM mykosen 22, Heft 12 (1979)

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confidence: 99%

Protoplasts of Microsporum Gypseum Conidia:* Protoplasten der Konidien von Microsporum gypseum

Weigl

Hejtmánek

1979

Mycoses

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Liposome‐protoplast interactions

Makins¹,

Holt²

1982

J. Chem. Technol. Biotechnol.

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A large number of different biological compounds can be encapsulated in liposomes and then delivered into the cytoplasm of cells by fusing these lipid vesicles with protoplasts. The essential properties of protoplasts and liposomes are briefly described. Examples of two liposome-protoplast interactions, involving the delivery of intermediates of antibiotic biosynthesis into fungi and DNA into Streptomyces are discussed. References are made to other experiments suggesting that liposomes may be useful in many areas of biotechnology.

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Protoplast Fusion Techniques in Fungi

Gnanam

2012

Laboratory Protocols in Fungal Biology

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Protoplasts of systemic dimorphic fungal pathogens: Histoplasma capsulatum and blastomyces dermatitidis

Cited by 16 publications

References 6 publications

Protoplasts of Microsporum Gypseum Conidia:* Protoplasten der Konidien von Microsporum gypseum

Protoplasts of Microsporum Gypseum Conidia:* Protoplasten der Konidien von Microsporum gypseum

Liposome‐protoplast interactions

Protoplast Fusion Techniques in Fungi

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