Protoplasts of Microsporum Gypseum Conidia:* Protoplasten der Konidien von Microsporum gypseum

Abstract

The technique of cell wall enzymatic lysis was used to prepare protoplastt from the microconidia of Microsporum gypseum. Cell wall elimination was immunologically verified. Protoplasts isolated from microconidia were found to be of the following two types: large with a vacuole and small without one. The small protoplasts regenerated on plates of osmotically stabilized complete medium with reduced agar content (0.5 O/o) and in stabilized liquid medium as well. The regeneration was discovered from the production of hyphae.* Herrn Prof. Dr. med. Dr. med. vet. 11. c. Hans Rieth zum 65. Geburtstag gewidmet. Das Tiefenantisepticum zur Lokaibehandlung bakterielier und mykoiischerAffektionen ! Mykohaug Plnselung Dermatomykosen (Epidermophytien), auch sekundar bakteriellinfizierte, rnykotische Ekreme (Interdigitalekzeme). Trychophytien Bestandteile: Bis-(2-rnethyl-4-arnlno-chinolyl-6)-carbarnid hydrochl. 0,0135 g. Acid. sal. 1.75 g. Aethanolarninborat 1 g, Acid. benz. 1,75 g. lsopropanol 50 g, Aqua dest. ad 100 g Handelsformen: Pinselflasche zu 30 rnl 4,60 DM, 50 rnl 6,35 DM und 100 rnl 10,lO DM rn. MwSt. SILWIE . CHEW. PHIRP. PRAPARITL , APOlHEKER MANFRED H I U C~ SWlTCRRl-WEDELFINEEM mykosen 22, Heft 12 (1979)