1984
DOI: 10.1128/aem.47.2.363-368.1984
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Protoplast Fusion of Trichoderma reesei , Using Immature Conidia

Abstract: Protoplast fusion of strains derived from Trichoderma reesei QM9414 and QM9136 and the segregation of the resulting fusants were studied. Combinations of protoplasts prepared from young conidia with double amino acid requirements, one of which was a common requirement and the other uncommon, were fused in the presence of polyethylene glycol 6000. Fusants were selected as regenerant colonies requiring only the commonly deficient amino acid. The frequency of fusion was 0.9 x 10-4 to 4.0 x 10-4 for the starting c… Show more

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Cited by 48 publications
(7 citation statements)
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“…Basal salt media containing enzyme substrate were used for the production of the studied enzymes. The basal medium [ 20 ] containing 100 μg ampicillin/mL was used as growth medium for protoplast isolation. Protoplast regeneration minimal medium (PRMM) was used for protoplast regeneration as described previously.…”
Section: Methodsmentioning
confidence: 99%
“…Basal salt media containing enzyme substrate were used for the production of the studied enzymes. The basal medium [ 20 ] containing 100 μg ampicillin/mL was used as growth medium for protoplast isolation. Protoplast regeneration minimal medium (PRMM) was used for protoplast regeneration as described previously.…”
Section: Methodsmentioning
confidence: 99%
“…Media. The media used in this study included the basal medium (BM) of Toyama et al (24), prepared as described by Stasz et al (20). This medium was also amended with 10% sucrose and used as a protoplast regeneration medium (PRM).…”
Section: Methodsmentioning
confidence: 99%
“…Successful fusion of protoplasts in Trichoderma spp. has been demonstrated (5,20,24). However, fusion between dissimilar strains of Trichoderma spp.…”
mentioning
confidence: 99%
“…Pyricularia oryzae (Tanaka et al, 1981), Rhizocionia solani (Hashiba and Yamada, 1984), and Selerotium rolfsii (Deshpande et al, 1987). Interspecific protoplast fusion between related species has been used to improve several industrially important traits in Aspergillus (Kirimura et al, 1986), PenieilUum (Anne and Eyssen, 1978), Trichoderma (Toyama et al, 1984), and yeasts (Pina et al, 1986).…”
Section: Introductionmentioning
confidence: 99%